Reconstruction and expression of chimeric anti-HBx antibody in Escherichia coli

Journal of Cancer Research and Clinical Oncology

Volumn 123, Issue 6, 1997, Pages 325-330

  Zhou, Ge ^a   Liu, Kang Da ^a   Tang, Zhao You ^a,d   Chen, Ya Hua ^b   Wu, Xiang Fu ^b   Schroeder, Claus H ^c  

^a FUDAN UNIVERSITY   (China)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^d ZHONGSHAN HOSPITAL OF FUDAN UNIVERSITY   (China)

Author keywords

Chimeric antibody; Hepatocellular carcinoma; Monoclonal antibody; Polymerase chain reaction; Targeting therapy

Indexed keywords

MONOCLONAL ANTIBODY HBX; UNCLASSIFIED DRUG;

ARTICLE; CHIMERA; ESCHERICHIA COLI; IMMUNOGLOBULIN VARIABLE REGION; LIVER CELL CARCINOMA; NONHUMAN; PRIORITY JOURNAL; RADIOIMMUNOTHERAPY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; ESCHERICHIA COLI; GENETIC VECTORS; HEPATITIS B ANTIGENS; HUMANS; IMMUNOGLOBULIN CONSTANT REGIONS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATORS;

EID: 0030750058     PISSN: 01715216     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004320050065     Document Type: Article

References (20)

1. Arakawa F, Haruno M, Kuroki M, Kanda H, Watanabe T, Misumi Y, Matsuoka Y (1993) Construction and expression of two mouse-human chimeric antibodies with high specificity and affinity for carcinoembryonic antigen. Hybridoma 12:365-379
2. Chomczynski P, Sacchi N (1987) Single-step method isolation of RNA by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159
3. Hilger C, Velhagen I, Zentgraf H, Schroder CH (1994) Diversity of hepatitis B virus X gene-related transcripts in hepatocellular carcinoma: a novel polyadenylation site on viral DNA. J Virol 65:4284-4291
4. Huston JS, Levinson D, Mudgett-Hunter M, Tai S, novotny J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber E, Crea R (1988) Protein engineering of antibody bind sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci USA 85:5879-5883
5. Jin BR, Ryu CJ, Park SS, Namgung U, Hong J, Han MH (1993) Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus. Mol Immunol 30:1647-1654
6. Li J, Tang ZY, Liu KD, Chen SL, Xu KP, Schroder CH (1994a) Radioimmunoimaging with monoclonal antibody to HBV X protein in nude mice bearing human hepatocellular carcinoma. Chin J Oncol 16:11-14 (in Chinese)
7. Li J, Tang ZY, Liu KD, Schroder CH (1994b) Preparation of monoclonal antibody directed against hepatitis B virus X protein and detection of reactive antigen in hepatocellular carcinoma (in Chinese). Natl Med J China 74:533-535
8. Li J, Tang ZY, Liu KD, Chen SL, Schroder CH (1995) Radioimmunoimaging of human hepatocellular carcinoma xenografts with 131-I-anti-HBx monoclonal antibody. J Exp Clin Cancer Res 14:25-30
9. Liu AY, Robinson RR, Hellstrom KE, Murray ED, Chang CP, Hellstrom I (1987) Chimeric mouse-human IgG1 antibody that can mediate lysis of cancer cells. Proc Natl Acad Sci USA 84:3439-3443
10. Lonberg N, Taylor LD, Harding FA (1994) Antigen-specific human antibodies from mice comprising four distinct genetic modifications Nature 368:856-859
11. McPherson MJ, Quirke P, Taylor GR (eds) (1992) PCR, a practical approach. Oxford University Press, Oxford, pp 229-230
12. Orlandi R, Gussow DH, Jones PT, Winter G (1986) Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. Proc Natl Acad Sci USA 86:3833-3837
13. Qin LX, Tang ZY, Liu KD, Xue Q (1995) Reconstitution of human immune system in SCID mouse with intraperitoneal injection of fetal liver cells. (in Chinese) Chin J Microbiol Immunol 15:272-275
14. Riechmann L, Clark M, Waldmann H, Winter G (1988) Reshaping human antibodies for therapy. Nature 332:323-327
15. Silverman GJ (1992) Human antibody responses to bacterial antigens: studies of a model conventional antigen and a proposed model B cell superantigen. Int Rev Immunol 9:57-78
16. Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Molecular cloning: a laboratory manual, 2nd edn, vols 4, 18. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 21-37; 47-58
17. Sandhu JS (1992) Protein engineering of antibodies. Crit Rev Biotechnol 12:437-462
18. Tang ZY, Zeng ZC, Liu KD, Yu YQ, Lu JZ, Xie H (1993a) Intrahepatic arterial I-131 antihepatocellular carcinoma (HCC) monoclonal antibody combined with hepatic artery ligation for treatmant of unresectable HCC. Antibody Immunoconj Radiopharm 6:167-175
19. Tang ZY, Liu KD, Fan Z, Lu JZ, Zhang YJ, Bao YM, Zeng ZC, Zhou D, Tang WY, Xia XL, Yang R, Li J, Wei C, Wu B, Lin ZY, Ma ZC, Zhou XD, Yu YQ, Yang BH, Zhao HY, Xie H, Schroder CH, Arosio P (1993b) A decade's studies on the immunotargeting therapy of hepatocellular carcinoma. Antibody Immunoconj Radiopharm 6:155-165
20. Verhoeyen M, Milstein C, Winter G (1988) Reshaping human antibodies: grafting human antilysozyme activity. Science 239:1534-1536