A comparison of three standard methods of identifying mast cells in endobronchial biopsies in normal and asthmatic subjects

Allergy: European Journal of Allergy and Clinical Immunology

Volumn 52, Issue 8, 1997, Pages 836-843

  Heaney, L G ^a,b,c   Leggett, P ^a   Maxwell, P ^a   Bharucha, H ^a,b   Ennis, M ^b  

^a ROYAL VICTORIA HOSPITAL   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^c BELFAST CITY HOSPITAL   (United Kingdom)

Author keywords

Asthma; Biopsy; Bronchoscopy; Mast cell; Method

Indexed keywords

FIXATIVE; TOLONIUM CHLORIDE;

ADULT; ARTICLE; ASTHMA; BRONCHOSCOPY; BRONCHUS BIOPSY; CELL COUNT; FEMALE; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; MALE; MAST CELL; PRIORITY JOURNAL;

EID: 0030748304     PISSN: 01054538     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1398-9995.1997.tb02155.x     Document Type: Article

References (20)

1. Azzawi M, Bradley B, Jeffery PK, et al. Identification of activated T lymphocytes and eosinophils in bronchial biopsies in stable atopic asthma. Am Rev Respir Dis 1990; 142:1407-13.
2. Bentley AM, Menz G, Storz C, et al. Identification of T lymphocytes, macrophages, and activated eosinophils in the bronchial mucosa in intrinsic asthma. Relationship to symptoms and bronchial responsiveness. Am Rev Respir Dis 1992;146:500-6.
3. Foresi A, Bertorelli G, Pesci A, Chetta A, Olivieri D. Inflammatory markers in bronchoalveolar lavage and in bronchial biopsy in asthma during remission. Chest 1990; 98:528-35.
4. Jeffery PK, Wardlaw AJ, Nelson FC, Collins JV, Kay AB. Bronchial biopsies in asthma. An ultrastructural, quantita-tive study and correlation with hyperreactivity. Am Rev Respir Dis 1989; 140:1745-53.
5. Bradley BL, Azzawi M, Jacobson M, et al. Eosinophils, T-lymphocytes, mast cells, neutrophils, and macrophages in bronchial biopsy specimens from atopic subjects with asthma: comparison with biopsy specimens from atopic subjects without asthma and normal control subjects and relationship to bronchial hyperresponsiveness. J Allergy Clin Immunol 1991;88:661-74.
6. Djukanovic R, Lai CK, Wilson JW, et al. Bronchial mucosal manifestations of atopy: a comparison of markers of inflammation between atopic asthmatics, atopic non-asthmatics and healthy controls. Eur Respir J 1992;5:538-44.
7. Holgate ST, Church MK. Asthma. The mast cell. Br Med Bull 1992;48:40-50.
8. Beasley R, Roche WR, Roberts JA, Holgate ST. Cellular events in the bronchi in mild asthma and after bronchial provocation. Am Rev Respir Dis 1989;139:806-17.
9. Pesci A, Foresi A, Bertorelli G, Chetta A, Olivieri D. Histochemical characteristics and degranulation of mast cells in epithelium and lamina propria of bronchial biopsies from asthmatic and normal subjects. Am Rev Respir Dis 1993;147:684-9.
10. Laitinen LA, Laitinen A, Haahtela T. Airway mucosal inflammation even in patients with newly diagnosed asthma. Am Rev Respir Dis 1993;147:697-704.
11. Jeffery PK, Godfrey RW, Adelroth E, Nelson F, Rogers A, Johansson SA. Effects of treatment on airway inflammation and thickening of basement membrane reticular collagen in asthma. A quantitative light and electron microscopic study. Am Rev Respir Dis 1992;145: 890-9.
12. Wingren U, Enerbäck L. Mucosal mast cells of the rat intestine: a reevaluation of fixation and staining properties with special reference to protein blocking and solubility of the granular glycosaminoglycan. Histochem J 1983;15: 571-82.
13. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986;1:307-10.
14. Nadel JA. Biology of mast cell tryptase and chymase. Ann N Y Acad Sci 1991;629:319-31.
15. Trigg CJ, Manolitsas ND, Jiahua W, et al. Placebocontrolled immunopathologic study of four months of inhaled corticosteroids in asthma. Am J Respir Crit Care Med 1994;150:17-22.
16. Olafsson JH, Roupe G, Enerhäck L. Dermal mast cells in mastocytosis: fixation, distribution and quantitation. Acta Derm Venereol 1986;66:16-22.
17. Otsuka H, Denburg J, Dolovich J. Heterogeneity of metachromatic cells in human nose: significance of mucosal mast cells. J Allergy Clin Immunol 1985;76:695-702.
18. Heard BE, Nunn AJ, Kay AB. Mast cells in human lungs. J Pathol 1989;157:59-63.
19. Shanahan F, MacNiven I, Dyck N, Denburg JA. Bienenstock J, Befus AD. Human lung mast cells: distribution and abundance of histochemically distinct populations, Int Arch Allergy Appl Immunol 1987;83:329-31.
20. Djukanovic R, Wilson JW, Britten KM, et al. Quantitation of mast cells and eosinophils in the bronchial mucosa of symptomatic atopic asthmatics and healthy control subjects using immunohistochemistry. Am Rev Respir Dis 1990;142: 863-71.