Efficient Non-PCR-mediated overlap extension of PCR fragments by exonuclease 'end polishing'

BioTechniques

Volumn 23, Issue 1, 1997, Pages 48-52

  Jespersen, Thomas ^a   Duch, Mogens ^a   Pedersen, Finn Skou ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; EXONUCLEASE;

ARTICLE; DNA DETERMINATION; GENE AMPLIFICATION; GENETIC RECOMBINATION; POLYMERASE CHAIN REACTION;

EID: 0030747959     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97231bm08     Document Type: Article

References (8)

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2. Clark, J.M. 1988. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
3. Costa, G.L. and M.P. Weiner. 1994. Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments. Nucleic Acids Res. 22:2423.
4. Horton, R.M., H.D. Hunt, S.N. Ho, J.K. Pullen and L.R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68.
5. Koo, H., A.M.C. Brown, R.J. Kaufman, C.M. Prorock, Y. Ron and J.P. Dougherty. 1992. A spleen necrosis virus-based retroviral vector which expresses two genes from a dicistronic mRNA. Virology 186:669-675.
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7. Suggs, S.V., T. Hirose, T. Miyake, E.H. Kawashima, M.J. Johnson, K. Itakura and R.B. Wallace. 1981. Use of synthetic oligodeoxyribonucleotides for the isolation of cloned DNA sequences, p. 683-693. In D.D. Brown and C.F. Fox (Eds.), Developmental Biology Using Purified Genes. Academic Press, New York.
8. Vallejo, A.N., R.J. Pogulis and L.R. Pease. 1994. Mutagenesis and synthesis of novel recombinant genes using PCR, p. 603-612. In C.W. Dieffenbach and G.S. Dveksler (Eds.), PCR Primer: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.