Sp1 binding is inhibited by mCpmCpG methylation

Gene

Volumn 195, Issue 1, 1997, Pages 67-71

  Clark, Susan J ^a,b   Harrison, Janet ^a   Molloy, Peter L ^b  

^a ROYAL PRINCE ALFRED HOSPITAL   (Australia)

^b CSIRO   (Australia)

Author keywords

CNG methylation; Gene regulation; Retinoblastoma; Transcription factor binding

Indexed keywords

CYTOSINE; DINUCLEOTIDE; TRANSCRIPTION FACTOR SP1;

ARTICLE; CONTROLLED STUDY; CPG ISLAND; DNA METHYLATION; GENE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; TUMOR SUPPRESSOR GENE;

BINDING SITES; CYTOSINE; DNA METHYLATION; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; RETINOBLASTOMA PROTEIN; SP1 TRANSCRIPTION FACTOR;

MAMMALIA;

EID: 0030746884     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00164-9     Document Type: Article

References (27)

1. Antequerra F., MacLeod D., Bird A.P. Specific protection of methylated CpGs in mammalian nuclei. Cell. 58:1989;509-517.
2. Ben-Hattar J., Beard P., Jiricny J. Cytosine methylation in CTF and Sp1 recognition sites of an HSV tk promoter: effects on transcription in vivo and on factor binding in vitro. Nucleic Acids Res. 17:1989;10179-10190.
3. Brandeis M., Frank D., Keshet I., Siegfried Z., Mendelsohn M., Nemes A., Temper V., Razin A., Cedar H. Sp1 elements protect a CpG island from de novo methylation. Nature. 371:1994;435-438.
4. Clark S.J., Harrison J., Frommer M. CpNpG methylation in mammalian cells. Nature Genet. 10:1995;20-27.
5. Clark S.J., Harrison J., Paul C.L., Frommer M. High sensitivity mapping of methylated cytosines. Nucleic Acids Res. 22:1994;2990-2997.
6. Fairall L., Schwabe J.W.R., Chapman L., Finch J.T., Rhodes D. The crystal structure of a two zinc-finger peptide reveals an extension of the rules for zinc-finger/DNA recognition. Nature. 366:1993;483-487.
7. BrCGCG. J. Biol. Chem. 257:1982;14686-14707.
8. Graessmann, M., Graessmann, A., 1993. DNA methylation, chromatin structure and the regulation of gene expression. In: Jost, J.P., Saluz, H.P. (Eds), DNA Methylation: Molecular Biology and Biological Significance. Birkhauser, Basel, pp 404-424.
9. Harrington M.A., Jones P.A., Imagawa M., Karin M. Cytosine methylation does not affect binding of transcription factor Sp1. Proc. Natl. Acad. Sci. USA. 85:1988;2066-2070.
10. Herman J.G., Merlo A., Mao L., Lapidus R.G., Issa J.-P.J., Davidson N.E., Sidransky D., Baylin S.B. Inactivation of the CDKN2/p16/MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers. Cancer Res. 55:1995;4525-4530.
11. Holler M., Westin G., Jiricny J., Schaffner W. Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated. Genes Dev. 2:1988;1127-1135.
12. Holliday R. Mechanisms for the control of gene activity during development. Biol. Rev. 65:1990;431-471.
13. Iguchi-Ariga S.M.M., Schaffner W. CpG methylation of the cAMP-responsive enhancer/promoter sequence TGACGTCA abolishes specific factor binding as well as transcriptional activation. Genes Dev. 3:1989;612-619.
14. Jones P.A., Buckley J.D. The role of DNA methylation in cancer. Adv. Cancer Res. 54:1990;1-23.
15. Kovesdi I., Reichel R., Nevins J.R. Role of adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control. Proc. Natl. Acad. Sci. USA. 84:1987;2180-2184.
16. Kudo S., Fukuda M. Transcriptional activation of human leukosialin (CD43) gene by Sp1 through binding to a GGGTGG motif. Eur. J. Biochem. 223:1994;319-327.
17. Kuwahara J., Yonezawa A., Futamura M., Sugiura Y. Binding of transcription factor Sp1 to GC box DNA revealed by footprinting analysis: different contact of three zinc fingers and sequence recognition mode. Biochemistry. 32:1993;5994-6001.
18. Letovsky J., Dynan W.S. Measurement of the binding of transcription factor Sp1 to a single GC box recognition sequence. Nucleic Acids Res. 17:1989;2639-2653.
19. Macleod D., Charlton J., Mullins J., Bird A.P. Sp1 sites in the mouse aprt gene promoter are required to prevent methylation of the CpG island. Genes Dev. 8:1994;2282-2292.
20. Meehan R.R., Lewis J.D., McKay S., Kleiner E.L., Bird A.P. Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs. Cell. 58:1989;499-507.
21. Nardelli J., Gibson T.J., Vesque C., Charnay P. Base sequence discrimination by zinc-finger DNA-binding domains. Nature. 349:1991;175-178.
22. Paul C.L., Clark S.J. Cytosine methylation: quantitation by automated genomic sequencing and GENESCAN™ analysis. BioTechniques. 21:1996;126-133.
23. Razin A., Cedar H. DNA methylation and gene expression. Microbiol. Rev. 55:1991;451-458.
24. Sakai T., Toguchida J., Ohtani N., Yandell D.W., Rapaport J.M., Dryja T. Allele-specific hypermethylation of the retinoblastoma tumor-suppressor gene. Am. J. Hum. Genet. 48:1991;880-888.
25. Stirzaker S.C., Millar D.S., Paul C.L., Warnecke P.M., Harrison J., Vincent P.C., Frommer M., Clark S.J. Extensive DNA methylation spanning the Rb promoter in retinoblastoma tumors. Cancer Res. 57:1997;. in press.
26. Thiesen H.J., Bach C. Target Detection Assay (TDA): a versatile procedure to determine DNA binding sites as demonstrated on SP1 protein. Nucleic Acids Res. 18:1990;3203-3209.
27. Watt F., Molloy P.L. Cytosine methylation prevents binding to DNA of a HeLa cell transcription factor required for optimal expression of the adenovirus major late promoter. Genes Dev. 2:1988;1136-1143.