Use of cell ELISA for the screening of neurotrophic activities on minor cell populations in retinal monolayer cultures

Journal of Neuroscience Methods

Volumn 75, Issue 2, 1997, Pages 199-205

  Fuhrmann, Sabine ^a   Kirsch, Matthias ^a   Wewetzer, Konstantin ^a   Hofmann, Hans Dieter ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Cell ELISA; Growth promoting activity; MAP2; Opsin; Photoreceptor cell

Indexed keywords

CILIARY NEUROTROPHIC FACTOR; GROWTH PROMOTOR; MICROTUBULE ASSOCIATED PROTEIN; OPSIN; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; CHICK EMBRYO; CONTROLLED STUDY; EMBRYO; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOCYTOCHEMISTRY; MONOLAYER CULTURE; NONHUMAN; PHOTORECEPTOR CELL; PRIORITY JOURNAL; RETINA CELL;

ANIMALS; CELL COUNT; CELLS, CULTURED; CHICK EMBRYO; CILIARY NEUROTROPHIC FACTOR; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOHISTOCHEMISTRY; MICROTUBULE-ASSOCIATED PROTEINS; NERVE GROWTH FACTORS; NERVE TISSUE PROTEINS; OPSIN; PHOTORECEPTORS; PIGMENT EPITHELIUM OF EYE; RETINA; RODS (RETINA);

EID: 0030743111     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(97)00073-3     Document Type: Article

References (22)

1. Adler R, Landa KB, Manthorpe M, Varon S. Cholinergic neuronotrophic factors: intraocular distribution of trophic activity for ciliary neurons. Science 1979;204:1434-6.
2. Alexi T, Hefti F. Trophic actions of transforming growth factor a on mesencephalic dopaminergic neurons developing in culture. Neuroscience 1993;55:903-18.
3. Barbin G, Manthorpe M, Varon S. Purification of the chick eye ciliary neuronotrophic factor (CNTF). J Neurochem 1984;43:1468-78.
4. Beck KD, Knüsel B, Hefti F. The nature of the trophic action of brain-derived neurotrophic factor, des(1-3)-insulin-like growth factor-1, and basic fibroblast growth factor on mesencephalic dopaminergic neurons developing in culture. Neuroscience 1993;52:855-66.
5. Doherty P, Dickson JG, Flanigan TP, Walsh FS. Quantitative evaluation of neurite outgrowth in cultures of human foetal brain and dorsal root ganglion cells using an enzyme-linked immunoadsorbent assay for human neurofilament protein. J Neurochem 1984;42:1116-22.
6. Fuhrmann S, Kirsch M, Hofmann H-D. Ciliary neurotrophic factor promotes chick photoreceptor development in vitro. Development 1995;121:2695-706.
7. Gaffar SA, Li Z, Epstein AL. A live cell enzyme-linked immunosorbent assay for detecting human hepatoma membrane antigens. Hybridoma 1989;8:331-6.
8. Hamburger V, Hamilton HL. A series of normal stages in the development of the chick embryo. J Morphol 1951;88:49-92.
9. Heller S, Huber J, Finn TP, Nishi R, Rohrer H. GPA and CNTF produces similar effects in sympathetic neurons but differ in receptor binding. Neuroreport 1993;5:357-60.
10. Heller S, Finn TP, Huber J, Nishi R, Geißen M, Püschel A, Rohrer H. Analysis of function and expression of the chick GPA receptor (GPARa) suggests multiple roles in neuronal development. Development 1995;121:2681-93.
11. Hofmann H-D. Development of cholinergic retinal neurons from embryonic chicken in monolayer cultures: Stimulation by glial cell-derived factors. J Neurosci 1988;8:1361-9.
12. Ip NY, Li Y, van de Stadt I, Panayotatos N, Alderson RF, Lindsay RM. Ciliary neurotrophic factor enhances neuronal survival in embryonic rat hippocampal cultures. J Neurosci 1991;11:3124-34.
13. Kirsch M, Fuhrmann S, Wiese A, Hofmann H-D. CNTF exerts opposite effects on in vitro development of rat and chick photoreceptors. Neuroreport 1996;7:697-700.
14. Leung DW, Parent AS, Cachianes G, Esch F, Coulombe JN, Nikolics K, Eckenstein FP, Nishi R. Cloning, expression during development, and evidence for release of a trophic factor for ciliary ganglion neurons. Neuron 1992;8:1045-53.
15. Molday RS. Monoclonal antibodies to rhodopsin and other proteins of rod outer segments. Prog Retinal Res 1989;8:173-209.
16. Nishi R, Berg DK. Two components from eye tissue that differentially stimulate the growth and development of ciliary ganglion neurons in cell culture. J Neurosci 1981;1:505-13.
17. Sedgwick JD, Czerninsky C. Detection of cell-surface molecules, secreted products of single cells and cellular proliferation by enzyme immunoasssay. J Immunol Methods 1992;150:159-75.
18. Tucker RP, Matus AI. Developmental regulation of two microtubule-associated proteins (MAP2 and MAP5) in the embryonic avian retina. Development 1987;101:535-46.
19. Varon S, Adler R. Trophic and specifying factors directed to neuronal cells. Adv Cell Neurobiol 1981;2:115-63.
20. Wewetzer K, Heiniger C, Seilheimer B. An improved cell-ELISA for the differential screening of antibodies against cell surface molecules of viable adherent Schwann cells. J Immunol Methods 1996;191:171-8.
21. Zhou L, Zhang S-R, Yue BYJT. Adhesion of human trabecular meshwork cells to extracellular matrix proteins. Invest Ophtalmol Vis Sci 1996;37:104-13.
22. Lowry OH, Rosenbrough NY, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.