Interaction between botulinum neurotoxin type a and ganglioside: Ganglioside inactivates the neurotoxin and quenches its tryptophan fluorescence

Toxicon

Volumn 35, Issue 8, 1997, Pages 1337-1340

  Kamata, Y ^a   Yoshimoto, M ^a   Kozaki, S ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BOTULINUM TOXIN; GANGLIOSIDE;

BINDING SITE; CONFORMATION; FLUORESCENCE; IONIC STRENGTH; MOLECULAR INTERACTION; PRIORITY JOURNAL; SHORT SURVEY; TOXIN ANALYSIS; TOXIN STRUCTURE;

EID: 0030742486     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(97)00027-5     Document Type: Article

References (14)

1. Binz, T., Kurazono, H., Wille, M., Frevert, J., Wernars, K. and Niemann, H. (1990) The complete sequence of botulinum neurotoxin type A and comparison with other clostridial neurotoxins. Journal of Biological Chemistry 265, 9153-9158.
2. Habarmann, E. and Dreyer, F. (1986) Clostridial neurotoxins: handling and action at the cellular and molecular level. Current Topics in Microbiology and Immunology 129, 93-179.
3. Kamata, Y., Kimura, Y. and Kozaki, S. (1994) Involvement of phospholipids in the intoxication mechanism of botulinum neurotoxin. Biochimica et Biophysica Acta 1199, 65-68.
4. Kondo, H., Shimizu, T., Kubonoya, M., Izumi, N., Takahashi, M. and Sakaguchi, G. (1984) Titration of botulinum toxins for lethal toxicity by intravenous injection into mice. Japanese Journal of Medical Science and Biology 37, 131-135.
5. TIb on the activities of Clostridium botulinum toxins. FEMS Microbiology Letters 21, 219-223.
6. Montecucco, C. and Schiavo, G. (1994) Mechanism of action of tetanus and botulinum neurotoxins. Molecular Microbiology 13, 1-8.
7. Niemann, H. (1991) Molecular biology of clostridial neurotoxins. In Source Book of Bacterial Protein Toxins, ed. J. Alouf and J. Freer, pp. 303-348. Academic Press, New York.
8. Sakaguchi, G. (1983) Clostridium botulinum toxins. Pharmacology and Therapeutics 19, 165-194.
9. Shone, C. C., Hambleton, P. and Melling, J. (1985) Inactivation of Clostridium botulinum type A neurotoxin by trypsin and purification of two tryptic fragments. European Journal of Biochemistry 151, 75-82.
10. Simpson, L. L. and Rapport, M. M. (1971) Ganglioside inactivation of botulinum toxin. Journal of Neurochemistry 18, 1341-1343.
11. Sugii, S. and Sakaguchi, G. (1975) Molecular construction of Clostridium botulinum type A toxins. Infection and Immunity 12, 1262-1270.
12. Svennerholm, L. (1957) Quantitative estimation of sialic acids. II. A calorimetric resorcinol-hydrophobic acid method. Biochimica et Biophysica Acta 24, 604-611.
13. Takamizawa, K., Iwamori, M., Kozaki, S., Sakaguchi, G., Tanaka, R., Takayama, H. and Nagai, Y. (1986) TLC-immunostaining characterization of Clostridium botulinum type A neurotoxin binding to gangliosides and free fatty acids. FEBS Letters 201, 229-232.
14. Teale, F. W. J. and Weber, G. (1957) Ultraviolet fluorescence of the aromatic amino acids. Biochemical Journal 65, 476-482.