Phosphorylation at a single site in the rat brain sodium channel is necessary and sufficient for current reduction by protein kinase A

Journal of Neuroscience

Volumn 17, Issue 16, 1997, Pages 6086-6093

  Smith, Raymond D ^a   Goldin, Alan L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

CAMP; Forskolin; Ion channel; Modulation; Phosphorylation; Protein kinase A; Site directed mutagenesis; Sodium channel; Xenopus oocytes

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; SODIUM CHANNEL;

ACTION POTENTIAL; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CHANNEL GATING; DEPOLARIZATION; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT; XENOPUS;

EID: 0030742101     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.17-16-06086.1997     Document Type: Article

References (21)

1. Auld VJ, Goldin AL, Krafte DS, Catterall WA, Lester HA, Davidson N, Dunn RJ (1990) A neutral amino acid change in segment IIS4 dramatically alters the gating properties of the voltage-dependent sodium channel. Proc Natl Acad Sci USA 87:323-327.
2. Chen T-C, Law B, Kondratyuk T, Rossie S (1995) Identification of soluble protein phosphatases that dephosphorylate voltage-sensitive sodium channels in rat brain. J Biol Chem 270:7750-7756.
3. Costa MR, Catterall WA (1984) Cyclic AMP-dependent phosphorylation of the alpha subunit of the sodium channel in synaptic nerve ending particles. J Biol Chem 259:8210-8218.
4. Costa MR, Casnellie JE, Catterall WA (1982) Selective phosphorylation of the alpha subunit of the sodium channel by cAMP-dependent protein kinase. J Biol Chem 257:7918-7921.
5. Frohnwiescr B, Chen L-Q, Schreibmayer W, Kallen RG (1997) Modulation of the human cardiac sodium channel α-subunit by cAMP-dependent protein kinase and the responsible sequence domain. J Physiol (Lond) 498:309-318.
6. + current in Xenopus oocytes. J Neurosci 12:3743-3752.
7. Goldin AL (1991) Expression of ion channels by injection of mRNA into Xenopus oocytes. Methods Cell Biol 36:487-509.
8. Hopp TP, Prickett KS, Price VL, Libby RT, March CJ, Cerretti DP, Urdal DL, Conlon PJ (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Biotechnology 6:1204-1210.
9. Kemp BE, Pearson RB (1990) Protein kinase recognition sequence motifs. Trends Biochem Sci 15:342-346.
10. Li M, West JW, Lai Y, Scheuer T, Catterall WA (1992) Functional modulation of brain sodium channels by cAMP-dependent phosphorylation. Neuron 8:1151-1159.
11. Li M, West JW, Numann R, Murphy BJ, Scheuer T, Catterall WA (1993) Convergent regulation of sodium channels by protein kinase C and cAMP-dependent protein kinase. Science 261:1439-1442.
12. + channel α subunit by cAMP-dependent protein kinase and phosphoprotein phosphatases. J Biol Chem 268:27355-27362.
13. Patton DE, Goldin AL (1991) A voltage-dependent gating transition induces use-dependent block by tetrodotoxin of rat IIA sodium channels expressed in Xenopus oocytes. Neuron 7:637-647.
14. Rossie S, Catterall WA (1987) Cyclic AMP-dependent phosphorylation of voltage-sensitive sodium channels in primary cultures of rat brain neurons. J Biol Chem 262:12735-12744.
15. 687. J Biol Chem 264:14220-14224.
16. Rossie S, Gordon D, Catterall WA (1987) Identification of an intracellular domain of the sodium channel having multiple cAMP-dependent phosphorylation sites. J Biol Chem 262:17530-17535.
17. 1 receptor modulates the voltage-gated sodium current in rat striatal neurones through a protein kinase A. J Physiol (Lond) 483:95-107.
18. Smith RD, Goldin AL (1992) Protein kinase A phosphorylation enhances sodium channel currents in Xenopus oocytes. Am J Physiol 236:C660-C666.
19. Smith RD, Goldin AL (1996) Phosphorylation of brain sodium channels in the I-II linker modulates channel function in Xenopus oocytes. J Neurosci 16:1965-1974.
20. Srinivasan Y, Elmer L, Davis J, Bennett V, Angelides K (1988) Ankyrin and spectrin associate with voltage-dependent sodium channels in brain. Nature 333:177-180.
21. + channel inactivation. Proc Natl Acad Sci USA 89:10910-10914.