Molecular characterization and expression of two putative protective excretory secretory proteins of Haemonchus contortus

Molecular and Biochemical Parasitology

Volumn 88, Issue 1-2, 1997, Pages 203-213

  Schallig, Henk D F H ^a   Van Leeuwen, Marianne A W ^a   Verstrepen, Babs E ^a   Cornelissen, Albert W C A ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Excretory secretory proteins; Haemonchus contortus; Recombinant expression

Indexed keywords

ANTIGEN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; GENE EXPRESSION; HAEMONCHUS CONTORTUS; IMMUNOBLOTTING; PARASITE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; SHEEP; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, HELMINTH; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, HELMINTH; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENES, HELMINTH; HAEMONCHIASIS; HAEMONCHUS; HELMINTH PROTEINS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SHEEP; SHEEP DISEASES; VACCINATION;

HAEMONCHUS CONTORTUS; OVIS ARIES;

EID: 0030740120     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(97)00093-5     Document Type: Article

References (27)

1. Düwel D. Resistance of endoparasites to antiparasitic drugs. Helminthologia 1987;24:141-9.
2. Jackson F. Anthelmintic resistance - the state of play. Br Vet J 1993;149:123-38.
3. Jasmer DP, McGuire TC. Protective immunity to a blood-feeding nematode (Haemonchus contortus) induced by parasite gut antigens. Infect Immun 1991;59:4412-7.
4. Jasmer DP, McGuire TC. Antigens with application towards immune control of blood-feeding parasitic nematodes. Br Vet J 1996;152:251-68.
5. Munn EA, Smith TS, Graham M, Greenwood CA, Tavernor AS, Coetzee G. Vaccination of merino lambs against haemonchosis with membrane-associated proteins from the adult parasite. Parasitol 1993;106:63-6.
6. Munn EA, Smith TS, Graham M, Tavernor AS, Greenwood CA. The potential value of integral membrane proteins in the vaccination of lambs against Haemonchus contortus. Int J Parasitol 1993;23:261-9.
7. Smith TS, Munn EA, Graham M, Tavernor AS, Greenwood CA. Purification and evaluation of the integral membrane protein H11 as a protective antigen against Haemonchus contortus. Int J Parasitol 1993;23:271-80.
8. Smith SK, Smith WD. Immunisation of sheep with an integral membrane glycoprotein complex of Haemonchus contortus and with its major polypeptide components. Res Vet Sci 1996;60:1-6.
9. Smith WD. Protection in lambs immunised with Haemonchus contortus gut membrane proteins. Res Vet Sci 1993;54:94-101.
10. Smith WD, Smith KS. Evaluation of aspects of the protection afforded to sheep immunised with a gut membrane protein of Haemonchus contortus. Res Vet Sci 1993;55:1-9.
11. Smith WD, Smith SK, Murray JM. Protection studies with integral membrane fractions of Haemonchus contortus. Parasite Immunol 1994;16:231-41.
12. Tavernor AS, Smith TS, Langford CF, Graham M, Munn EA. Immune response of Clun Forest sheep to vaccination with membrane glycoproteins from Haemonchus contortus. Parasite Immunol 1992;14:671-5.
13. Schallig HDFH, van Leeuwen MAW. Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens. Parasitology 1997;114:293-9.
14. Schallig HDFH, van Leeuwen MAW, Hendrikx WML. Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus. Parasitology 1994;108:351-7.
15. Towbin H, Staehlin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
16. Maizels RM, Blaxter ML, Robertson BD, Selkirk ME. Parasite Antigens Parasite Genes. A laboratory manual for molecular parasitology. Cambridge, UK: Cambridge University Press, 1991.
17. Sanger F, Nicklen S, Coulson AC. DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 1977;74:5463-7.
18. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403-10.
19. Pearson WR, Lipman DJ. Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 1988;85:2444-8.
20. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: a Laboratory Manual. 2nd ed. New York: Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989.
21. Schallig HDFH, van Leeuwen MAW, Hendrikx WML. Isotype specific serum antibody responses of sheep to Haemonchus contortus antigens. Vet Parasitol 1995;56:149-62.
22. von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 1986;14:4683-90.
23. Kornfield R, Kornfield S. Assembly of asparagine-linked oligosaccharides. Ann Rev Biochem 1985;54:631-64.
24. Dopheide TAA, Tachedjian M, Phillips C, Frenkel MJ, Wagland BM, Ward C. Molecular characterization of a protective, 11 kDa excretory-secretory protein from the parasitic stages of Trichostrongylus colubriformis. Mol Biochem Parasitol 1991;45:101-8.
25. Savin KW, Dopheide TAA, Frenkel MJ, Wagland BM, Grant WN, Ward CW. Characterization, cloning and host-protective activity of a 30-kilodalton glycoprotein secreted by the parasitic stages of Trichostrongylus colubriformis. Mol Biochem Parasitol 1990;41:167-76.
26. Hawdon JM, Jones BF, Hoffman DR, Hotez PJ. Cloning and characterization of Ancylostoma-secreted protein. J Biol Chem 1996;271:6672-8.
27. Takáts C, Schallig HDFH, van Leeuwen MAW, Hendrikx WML. Immune responses of sheep to microdissected parts of Haemonchus contortus. Int J Parasitol 1995;25:857-60.