Structure and expression of the human SM22α gene, assignment of the gene to chromosome 11, and repression of the promoter activity by cytosine DNA methylation

Journal of Biochemistry

Volumn 122, Issue 1, 1997, Pages 157-167

  Yamamura, Hisako ^a   Masuda, Hiroaki ^a,c   Ikeda, Wataru ^a,b   Tokuyama, Tai ^b   Takagi, Michiko ^a   Shibata, Nobuhiko ^a   Tatsuta, Masaharu ^a   Takahashi, Katsuhito ^a  

^a OSAKA MEDICAL CENTER FOR CANCER AND CARDIOVASCULAR DISEASES   (Japan)

^b KANSAI UNIVERSITY   (Japan)

^c GUNMA UNIVERSITY   (Japan)

Author keywords

Calponin; CArG box; CpG; Differentiation; DNA methylation; SM22 gene; Smooth muscle; actin

Indexed keywords

CYTOSINE; DNA; LUCIFERASE; MESSENGER RNA; MUSCLE PROTEIN; TRANSCRIPTION FACTOR SP1;

ARTICLE; ATHEROSCLEROTIC PLAQUE; BINDING SITE; CHROMOSOME 11Q; CONTROLLED STUDY; CPG ISLAND; DNA FLANKING REGION; DNA METHYLATION; DNA SEQUENCE; DNA TRANSFECTION; EXON; FIBROBLAST; GENE EXPRESSION; GENE MAPPING; GENE REPRESSION; GENE STRUCTURE; HUMAN; HUMAN CELL; HUMAN TISSUE; INTRON; PROMOTER REGION; REPORTER GENE; SEQUENCE HOMOLOGY; SKELETAL MUSCLE; SMOOTH MUSCLE FIBER; START CODON; STRUCTURE ACTIVITY RELATION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

MURINAE;

EID: 0030740006     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021722     Document Type: Article

References (44)

1. Sappino, A.P., Schurch, W., and Gabbiani, G. (1990) Differentiation repertoire of fibroblastic cells: Expression of cytoskeletal proteins as marker of phenotypic modulations. Lab. Invest. 63, 144-161
2. Owens, G.K. (1995) Regulation of differentiation of vascular smooth muscle cells. Physiol. Rev. 75, 487-517
3. Shah, N.M., Groves, A.K., and Anderson, D.J. (1996) Alternative neural crest cell fates are instructively promoted by TGFβ superfamily members. Cell 85, 331-343
4. Almendral, J.M., Santaren, J.F., Perera, J., Zerial, M., and Bravo, R. (1989) Expression, cloning and cDNA sequence of a fibroblast serum-regulated gene encoding a putative actin-associated protein (p27). Exp. Cell Res. 181, 518-530
5. Solway, J., Seltzer, J., Samaha, F.F., Kim, S., Alger, L.E., Niu, Q., Morriesey, E.E., Ip, H.S., and Parmacek, M.S. (1995) Structure and expression of a smooth muscle cell-specific gene, SM22α. J. Biol. Chem. 270, 13460-13469
6. Kemp, P.R., Osbourn, J.K., Grainger, D.J., and Metcalf, C. (1995) Cloning and analysis of the promoter region of the rat SM22α gene. Biochem. J. 310, 1037-1043
7. Takahashi, K. and Nadal-Ginard, B. (1991) Molecular cloning and sequence analysis of smooth muscle calponin. J. Biol. Chem. 266, 13284-13288
8. Li, L., Miano, J.M., Cserjesi, P., and Olson, E.N. (1996) SM22α, a marker of adult smooth muscle, is expressed in multiple myogenic lineages during embryogenesis. Circ. Res. 78, 188-195
9. Duband, J.-L., Gimona, M., Scatena, S., Sartore, S., and Small, J.V. (1993) Calponin and SM22 as differentiation markers of smooth muscle: spatiotemporal distribution during avian embryonic development. Differentiation 55, 1-11
10. Li, L., Miano, J.M., Mercer, B., and Olson, E.N. (1996) Expression of the SM22α promoter in transgenic mice provides evidence for distinct transcriptional regulatory programs in vascular and visceral smooth muscle cells. J. Cell Biol. 132, 849-859
11. Kim, S., Ip, H.S., Lu, M.M., Clendenin, C., and Parmacek, M.S. (1997) A serum response factor-dependent transcriptional regulatory program identifies distinct smooth muscle cell sublineages. Mol. Cell Biol. 17, 2266-2278
12. Lees-Miller, J.P., Heeley, D.H., Smillie, L.B., and Kay, C.M. (1987) Isolation and characterization of an abundant and novel 22-kDa protein (SM22) from chicken gizzard smooth muscle. J. Biol. Chem. 262, 2988-2993
13. Takahashi, K., Hiwada, K., and Kokubu, T. (1987) Occurrence of anti-gizzard p34K antibody cross-reactive components in bovine smooth muscles and non-smooth muscle tissues. Life Sci. 41, 291-296
14. Towbin, H., Staehlin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354
15. Pearlstone, J.R., Weber, M., Lees-Miller, J.P., Carpenter, M.R., and Smillie, L.B. (1987) Amino acid sequence of chicken gizzard smooth muscle SM22α. J. Biol. Chem. 262, 5985-5991
16. Stallcup, M.R. and Washington, L.D. (1983) Region-specific initiation of mouse mammary tumor virus RNA synthesis by endogenous RNA polymerase II in preparations of cell nuclei. J. Biol. Chem. 258, 2802-2807
17. Tomatsu, S. and Sasaki, Y. (1989) The organization and the complete nucleotide sequence of the human NADH-cytochrome b5 reductase gene. Gene 80, 353-361
18. Maruyama, K. and Sugano, S. (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 138, 171-174
19. Heng, H.H.Q., Squire, J., and Tsui, L.-C. (1992) High resolution mapping of mammalian genes by in situ hybridization to free chromatin. Proc. Natl. Acad. Sci. USA 89, 9509-9513
20. Rothman, A., Kulik, T.J., Taubman, M.B., Berk, B.C., Smith, C.W.J., and Nadal-Ginard, B. (1992) Development and characterization of a cloned rat pulmonary arterial smooth muscle cell line that maintains differentiated properties through multiple subcultures. Circulation 86, 1977-1986
21. Thweatt, R., Lumpkin, C.K., Jr., and Goldstein, S. (1992) A novel gene encoding a smooth muscle protein is overexpressed in senescent human fibroblasts. Biochem. Biophys. Res. Commun. 187, 1-7
22. Smale, S.T. and Baltimore, D. (1989) The "initiator" as a transcription control element. Cell 57, 103-113
23. Briggs, M.R., Kadonaga, J.T., Bell, S.P., and Tjian, R. (1986) Purification and biochemical characterization of the promoter-specific transcription factor, Sp1. Science 234, 47-52
24. Gossett, L.A., Kelvin, D.J., Sternberg, E.A., and Olson, E.N. (1989) A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes. Mol. Cell. Biol. 9, 5022-5033
25. Shanahan, C.M., Cary, N.R.B., Metcalf, J.C., and Weissberg, P.L. (1994) High expression of genes for calcification-regulating proteins in human atherosclerotic plaques. J. Clin. Invest. 93, 2393-2402
26. Razin, A. and Kafri, T. (1994) DNA methylation from embryo to adult in Progress in Nucleic Acid Research and Molecular Biology (Cohn, W.E. and Moldave, K., eds.) Vol. 48, pp. 53-81, Academic Press, San Diego
27. Doerfler, W. (1983) DNA methylation and gene activity. Annu. Rev. Biochem. 52, 93-124
28. Bird, A. (1992) The essentials of DNA methylation. Cell 70, 5-8
29. Watt, F. and Molly, P.L. (1988) Cytosine methylation prevents binding to DNA of a Hela cell transcription factor required for optimal expression of the adenovirus major late promoter. Genes Dev. 2, 1136-1143
30. Boyes, J. and Bird, A. (1992) Repression of genes by DNA methylation depends on CpG density and promoter strength: Evidence for involvement of a methyl-CpG binding protein. EMBO J. 11, 327-333
31. Muscat, G.O., Gustafson, T.A., and Treisman, R. (1988) A common factor regulated skeletal and cardiac α-actin gene transcription in muscle. Mol. Cell Biol. 8, 4120-4133
32. Treisman, R. (1985) Structure and function of serum response factor in Transcriptional Regulation (McKnight, S.L. and Yamamoto, K.R., eds.) pp. 881-905, Cold Spring Harbor Laboratory Press, New York
33. Kim, J.-H., Bushel, P.R., and Kumar, C.C. (1993) Smooth muscle α-actin promoter activity is induced by serum stimulation of fibroblast cells. Biochem. Biophys. Res. Commun. 190, 1115-1121
34. Simonson, M.S., Walsh, K., Kumar, C.C., Bushel, P., and Herman, W.H. (1995) Two proximal CArG elements regulate SM α-actin promoter, a genetic marker of activated phenotype of mesangial cells. Am. J. Physiol. 268, F760-F769
35. Yano, H., Hayashi, K., Momiyama, T., Saga, H., Haruna, M., and Sobue, K. (1995) Transcriptional regulation of the chicken caldesmon gene. J. Biol. Chem. 270, 23661-23666
36. Brandeis, M., Frank, D., Keshet, L., Siegfried, Z., Mendelsohn, M., Nemes, A., Temper, V., Razin, A., and Cedar, H. (1994) Sp1 elements protect a CpG island from de novo methylation. Nature 371, 435-438
37. Macleod, D., Charlton, J., Mullins, J., and Bird, A.P. (1994) Sp1 sites in the mouse aprt gene promoter are required to prevent methylation of the CpG island. Genes Dev. 8, 2282-2292
38. Boyes, J. and Felsenfeld, G. (1996) Tissue-specific factors additively increase the probability of the all-or-none formation of a hypersensitive site. EMBO J. 15, 2496-2507
39. Felsenfeld, G. (1996) Chromatin unfolds. Cell 86, 13-19
40. Keshet, I., Lieman-Hurwitz, J., and Cedar, H. (1986) DNA methylation affects the formation of active chromatin. Cell 44, 535-543
41. Takahashi, K., Tazunoki, T., Okada, T., Ohgami, K., Miwa, T., Miki, A., and Shibata, N. (1996) The 5′-flanking region of the human smooth muscle cell calponin gene contains a cis-acting domain for interaction with a methylated DNA-binding transcription repressor. J. Biochem. 120, 18-21
42. Takahashi, K., Masuda, H., Takagi, M., Eshita, Y., and Shibata, N. (1995) Structure and expression of vascular smooth muscle calponin in Regulation of the Contractile Cycle in Smooth Muscle (Nakano, T. and Hartshorne, D.J., eds.) pp. 201-209, Springer-Verlag, Tokyo
43. Winder, S.J. and Walsh, M.P. (1996) Calponin. Curr. Topics Cell. Regul. 34, 33-61
44. Gimona, M. and Small, J.V. (1995) Calponin in Biochemistry of Smooth Muscle Contraction (Barany, M., ed.) pp. 91-103, Academic Press, New York