In vitro toxicity and formation of early conjugates in Caco-2 cell line treated with clenbuterol, salbutamol and isoxsuprine

European Journal of Drug Metabolism and Pharmacokinetics

Volumn 22, Issue 2, 1997, Pages 173-178

  Stammati, A ^a   Badino, P ^b   De Angelis, I ^a   Re, G ^b   Vincentini, O ^a   Zanetti, C ^a   Zucco, F ^c   Brambilla, G ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b UNIVERSITY OF TURIN   (Italy)

^c CNR   (Italy)

Author keywords

Beta agonists; Caco 2 cells; Cytotoxicity; Metabolism

Indexed keywords

BETA ADRENERGIC RECEPTOR STIMULATING AGENT; CLENBUTEROL; GLUCURONOSYLTRANSFERASE; ISOXSUPRINE; SALBUTAMOL;

ARTICLE; CELL STRAIN CACO 2; DRUG CONJUGATION; DRUG CYTOTOXICITY; HUMAN; HUMAN CELL;

EID: 0030739696     PISSN: 03787966     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03189802     Document Type: Article

References (27)

1. Buttery P.J., Dawson J.M. (1987) : The mode of action of beta-agonists as manipulators of carcass composition. In: Hanrahan J.P. (Ed) Beta-agonists and their effects on animal growth and carcass composition. London: Elsevier. 13-28.
2. Levy P., Robert A., Picard J. (1988) : Biosynthesis of glycosaminoglycans in the human colonic tumor cell line Caco-2: structural changes occuring with the morphological differentiation of the cells. Biol. Cell, 62, 255-264.
3. Levy P., Cherqui G., Robert A., Wicek D., Picard J. (1989) : Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2. Experientia, 45, 588-591.
4. Matsumoto H., Erickson R.H., Gum J.R., Yoshioka M., Gum E., Kim Y.S. (1990) : Biosynthesis of alkaline phosphatase during differentiation of the human colon cancer cell line Caco-2. Gastroenterology, 98, 1199-1207.
5. Boulenc X., Bourrie M., Fabre I., Roque C., Joyeux H., Berger Y., Fabré G. (1992) : Regulation of cytochrome P 450 IA1 gene expression in a human intestinal cell line, Caco-2. J. Pharm. Exp. Ther., 263, 1471-1477.
6. Peters H.N., Roelofs M.J. (1989) : Time-dependent activity and expression of glutathione S-tranferases in the human colon adenocarcinoma cell line Caco-2. Bioehem. J., 264, 613-616.
7. Smith P. (1986) : Gastrointestinal physiology. In: Rozman O.H. (Ed) Gastrointestinal toxicology. Amsterdam: Elsevier, 1-28.
8. Pompa G., Caloni F., Montana M., Pasqualucci C. (1994) : Prolonged presence of isoxsuprine in equine serum after oral administration. Xenobiotica, 24, 339-346.
9. Re G., Badino P., Novelli A., Girardi C. (1995) : Identification of b-adrenoceptor subtypes in bovine ovarian and myometrial cell membranes. Br. Vet. J., 151, 567-578.
10. Lowry O.H., Rosebrough N.J., Farr A., Randall R.J. (1951) : Protein measurements with the Folin reagent. J. Biol. Chem., 193, 265-275.
11. 125I]-iodocyanopindolol? J. Receptor Res., 12, 369-387.
12. Law M.P. (1993) : Demonstration of the suitability of CGP-12177 for in vivo studies of β-adrenoceptors. Br. J. Pharmacol., 109, 1101-1109.
13. 2-adrenergic receptor gene in pregnant rat myometrium. J. Biol. Chem., 267, 7975-7978.
14. Lemoine H. (1992) : β-Adrenoceptor ligands: characterization and quantification of drug effects. Quant. Struct. Act. Relat., 11, 211-218.
15. Scatchard G. (1949) : The attraction of proteins for small molecules and ions. Ann. NY Acad. Sci., 51, 660-669.
16. Tallarida R.J., Murray R.B. (1987) : Manual of pharmacologic calculations with computer programs, 2nd edn. New York: Springer, 110-128.
17. Liliemblum K. (1982) : Differential induction of rat liver microsomal UDP-glucuronyltransferase activities by various inducing agents: Biochem. Pharmacol., 31, 907-913.
18. Koster A., Meewisse C., Noordhoek J. (1984) : Glucuronidation in rat intestinal cells. Dependence of glucose supply and resistance to inhibition by ethanol and fasting. Arch. Toxicol., 55, 123-126.
19. Bjorge S., Hamelehle K.L., Homan R., Rose S.E., Turluck D., Scott Wright D. (1991) : Evidence for glucuronide conjugation of p-nitrophenol in the Caco-2 cell model. Pharm. Res., 8, 1441-1443.
20. Sergent-Engelen T., Delistrie V., Schneider Y-J. (1993) : Phase I and II biotransformations in living Caco-2 cells cultivated under serum-free conditions. Biochem. Pharmacol., 46, 1393-1401.
21. Peters W., Roelofs H. (1992) : Biochemical characterization of resistance to mitoxantrone and adriamycin in Caco-2 human colon adenocarcinoma cells: a possible role of glutathione-S-transferase. Cancer Res., 52, 1886-1890.
22. Abid A., Bouchon I., Siest G., Sabolovic N. (1995) : Glucuronidation in the Caco-2 human intestinal cell line: induction of UDP-glucuronosyltransferase 1*6. Biochem. Pharmacol., 50, 557-561.
23. Morgan D., Paul J.D., Richmond B.H., Wilson-Evered E., Ziccone S.P.(1986) : Pharmacokinetics of intravenous and oral salbutamol and its sulphate conjugate. Br. J. Clin. Pharmacol., 22, 587-593.
24. Matthews N.S., Gleed R.D., Short C.E., Burrows K. (1986) : Cardiovascular and pharmacokinetic effects of isoxsuprine in the horse. Am. J. Vet. Res., 47, 2130-2133.
25. Witkamp R. (1996) : Pharmacological and toxicological properties of β-agonists. In: Enne G., Kuiper H.A., Valentini A. (Eds) Residues of veterinary drugs and mycotoxins in animal products. New methods for risk assessment and quality control. Proceedings of the first electronic mail conference, Wageningen Pers, 113-123.
26. 3H: a new bronchodilator in rat, rabbit, dog and man. Eur. J. Pharmacol., 14, 183-199.
27. Pidgeon C., Ong S., Liu H. et al. (1995) : IAM chromatography: an in vitro screen for predicting drug membrane permeability. J. Med. Chem., 38, 590-594.