DNA content, interphase AgNOR-area, number of 3HrDNA hybridization signals and the methylation level in coding rDNA sequence in different organs of Lupinus luteus L.

Genetica

Volumn 99, Issue 1, 1997, Pages 67-72

  Sakowicz, T ^a   Olszewska, M J ^a  

^a UNIVERSITY OF LODZ   (Poland)

Author keywords

AgNOR area; DNA endoreplication; Methylation; rDNA amount

Indexed keywords

LUPINUS LUTEUS;

EID: 0030739280     PISSN: 00166707     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02259499     Document Type: Article

References (32)

1. Becker, T.A. & W. Nagl, 1994. Disproportionate numbers of rDNA loci in diploid and polyploid testis nuclei of Gerris najas detected by fluorescence in situ hybridization. Cell Biol. Intern. 18: 245-255.
2. Damsz, B. & P. Luchniak, 1988. Nuclear DNA endoreplication and plastid index in mesophyll of some dicotyledonous species. Acta Soc. Bot. Pol. 57: 303-316.
3. Delgado, M., L. Morais-Cecilio, N. Neves, R.N. Jones & W. Viegas, 1995. The influence of B chromosomes on rDNA organization in rye interphase nuclei. Chromosome Res. 3: 484-491.
4. Dellaporta, S.K., J. Wood & J.B. Hicks, 1983. Plant DNA micropreparation: version II. Plant Mol. Biol. Rep. 1: 19-21.
5. Delseny, M., M. Laroche & P. Penon, 1984. Methylation pattern of radish (Raphanus sativus) nuclear ribosomal RNA genes. Plant Physiol. 76: 627-632.
6. Derenzini, M., D. Trere, P. Chieco & C. Melchiorri, 1994. Interphase AgNOR quantity is not related to DNA content in 11 established human cancer cell lines. Exp. Cell Res. 211: 282-285.
7. Ellis, T.H.N., M. Delseny, D. Lee & K.W. Burcham, 1989. Methylated and undermethylated rDNA repeats are interspaced at random in two higher plant species. Plant Mol. Biol. 14: 73-80.
8. Finnegan, E.J., R.J.S. Brettel & E.S. Dennis, 1993. The role of DNA methylation in the regulation of plant gene expression, pp. 218-261 in DNA Methylation: Molecular Biology and Biological Significance, edited by J.B. Jost & H.P. Saluz. Birkhauser Verlag, Basel, Boston, Berlin.
9. Flavell, R.B., M. O'Dell, R. Sardana & S. Jackson, 1993. Regulatory DNA of ribosomal genes and control of nucleolus organizer activity in wheat. Crop Sci. 85: 889-894.
10. Flavell, R.B., M. O'Dell & W.F. Thompson, 1988. Regulation of cytosine methylation in ribosomal DNA and nucleolus organizer expression in wheat. J. Mol. Biol. 204: 523-534.
11. Gerlach, W.L. & J.R. Bedbrook, 1979. Cloning and characterization of ribosomal RNA gene from wheat and barley. Nucl. Acid Res. 7: 1869-1885.
12. Hemleben, Y, M. Ganal, J. Gerstner, K. Schiebel & R.A. Torres, 1988. Organization and length heterogeneity of plant ribosomal genes, pp. 371-383 in Architecture of Eukaryotic Genes, edited by G. Kahl. VCH Verlagsgesellschaft mGH, Wenheim.
13. Herrera, A.M. & M.O.J. Olson, 1986. Association of protein C23 with rapidly labelled nucleolar RNA. Biochemistry 25: 6258-6264.
14. Highett, M.J., D.J. Rawlins & P.J. Shaw, 1993. Different patterns of rDNA distribution in Pisum sativum nucleoli correlate with different level of nucleolar activity. J. Cell Sci. 104: 843-852.
15. Howell, M.H. & D.A. Black, 1980. Controlled silver-staining nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014-1015.
16. Kwiatkowska, M., A.K. Kononowicz & K. Janas, 1987. Observations on rRNA gene replication in Chara vulgaris evaluated by in situ [3H] rRNA-DNA hybridization. J. Cell Sci. 88: 407-414.
17. Le Gal, M.F., F.M. Lecocq & J.N. Hallett, 1986. The reserve proteins of mature cotyledons of Lupinus albus var. Lucky.II. Relationship with the nuclear DNA content. Protoplasma 130: 128-137.
18. Leitch, A.R., W. Mosgoller, M. Shi & J.S. Heslop-Harrison, 1992. Different patterns of rDNA organization at interphase in nuclei of wheat and rye. J. Cell Sci. 101: 751-757.
19. Maluszyńska, J., A.R. Leitch, J.J. Leitch, T. Schwarzacher & J.S. Heslop-Harrison, 1991. In situ hybridization to chromosomes of Arabidopsis thaliana. Arabidopsis: The complete guide, version 10. Plant Sci. Res. Colney Lane, Norwich WR4 7VH, U.K.
20. Martini, G., M. O'Dell & R.B. Flavell, 1982. Partial inactivation of wheat nucleolus organisers by the nucleolus organiser from Aegilops umbellata. Chromosoma 84: 687-700.
21. Moreno, F.J., R.M. Rodrigo, F. Garcia-Navarro & G. Garcia- Herdugo, 1989. Nucleolar component behaviour in plant cells under different physiological conditions. A morphological, cytochemical and quantitive study. Biol. Cell 65: 67-74.
22. Moreno, F.J., R.M. Rodrigo & G. Garcia-Herdugo, 1990. Ag-NOR proteins and rDNA transcriptional activity in plant cells. J. Histochem. Cytochem. 38: 1879-1887.
23. Olszewska, M.J., A. Bilecka, A.K. Kononowicz & P. Kolodziejczyk, 1988. Relationship between heterochromatin and repetitive DNA content and the dynamics of nuclear DNA endoreplication in root parenchyma cells. Biol. Zent. bl. 107: 311-325.
24. Olszewska, M.J. & A.K. Kononowicz, 1979. Activities of DNA polymerases and RNA polymerases detected in situ in growing and differentating cells of root cortex. Histochemistry 59: 311-323.
25. 3H rRNA/DNA hybridization and silver staining of NORs during growth and differentiation of root cortex cells in the presence or absence of DNA endoreplication, pp. 53-57 in Structure and Function of Plant Roots, edited by R. Brouwer et al. Martinus Nijhoff/Dr. W. Junk, The Hague, Boston, London.
26. Olszewska, M.J. & A.B. Legocki, 1989. Changes in DNA content during rhizobial nodule development in Lupinus luteus L.I. Cytophotometry and autoradiography. Biol. Zent. bl. 108: 221-230.
27. Rafalski, A., M. Wiewiórowski & D. Soll, 1983. Organization of ribosomal DNA in yellow lupine (Lupinus luteus) and sequence of the 5.8S RNA gene. FEBS Let. 152: 241-245.
28. Roussel, P. & D. Hernandez-Verdun, 1994. Identification of Ag- NOR proteins, markers of proliferation related to ribosomal gene activity. Exp. Cell Res. 214: 464-472.
29. Sambrook, J., E.F. Fritsch, T. Maniatis, 1988. Molecular cloning: A laboratory Manual, 2nd Ed.; Vol. II, Cold Spring Harbor, New York, pp. 9.1-9.62.
30. Sardana, R., M. O'Dell & R. Flavell, 1993. Correlation between the size of the intergenic regulatory region, the status of cytosine methylation of rRNA genes and nucleolar expression in wheat. Mol. Gen. Genet. 236: 155-162.
31. Thompson, W.F., R.B. Flavell, J.C. Watson & L.S. Kaufmann, 1988. Chromatin structure and expression of plant ribosomal genes, pp. 385-396 in Architecture of Eukaryotic Genes, edited by G. Khal. VCH Verlagsgesell-schaft mGH, Weinheim.
32. Torres-Ruiz, R.A. & V. Hemleben, 1994. Pattern and degree of methylation in ribosomal RNA genes of Cucurbita pepo L. Plant. Mol. Biol. 26: 1167-1179.