Cycle sequencing on large DNA templates

BioTechniques

Volumn 23, Issue 6, 1997, Pages 1034-1036

  Fajas, Lluis ^a   Staels, Bart ^a   Auwerx, Johan ^a,b  

^a INSERM   (France)

^b INSTITUT PASTEUR DE LILLE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; BACTERIOPHAGE P1; CHROMOSOME; CLONING VECTOR; COSMID; DNA SEQUENCE; DNA TEMPLATE; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 0030732357     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97236bm14     Document Type: Article

References (5)

1. Birnboim, H.C. 1983. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 100:243-255.
2. Ioannou, P.A., C.T. Amemiya, J. Garnes, P.M. Kroisel, H. Shizuya, C. Chen, M.A. Batzer and P.J. de Jong. 1994. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nat. Genet. 6:84-89.
3. Obermaier, B. and H. Domdey. 1992. Automated sequencing and mapping of cosmid DNA with fluorescently-labeled dideoxynucleotide terminators. BioTechniques 13:46-47.
4. Shizuya, H., B. Birren, U.J. Kim, V. Mancino, T. Slepak, Y. Tachiiri and M. Simon. 1992. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89: 8794-8797.
5. Sternberg, N., J. Ruether and K. de Riel. 1990. A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy. Proc. Natl. Acad. Sci. USA 87:4645-4649.