Human keratinocyte models: Assessment of cell adhesion and dermotoxicity using fluorescent probes

Toxicology in Vitro

Volumn 11, Issue 5, 1997, Pages 601-607

  Braut Boucher, F ^a   Pichon, J ^a   Wantyghem, J ^a   Muriel, M P ^a   Giner, M ^a   Font, J ^a   Aubery, M ^a  

^a INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

2,4 DINITROPHENOL; DODECYL SULFATE SODIUM; EPIDERMAL GROWTH FACTOR; FLUORESCENT DYE; GAMMA INTERFERON; PHORBOL 13 ACETATE 12 MYRISTATE;

ARTICLE; CELL ADHESION; CELL DIFFERENTIATION; CELL INTERACTION; CELL STRUCTURE; CYTOTOXICITY; FLUORESCENCE; HUMAN; HUMAN TISSUE; KERATINOCYTE; LYMPHOCYTE; SKIN TOXICITY;

EID: 0030732013     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(97)00067-2     Document Type: Article

References (28)

1. 5-integrins by epidermal keratinocytes and non differentiating keratinocytes. Journal of Cell Biology 115, 829-841.
2. Balls M. and Clothier R. H. (1991) Comments on the scientific validation and regulatory acceptance of in vitro toxicity tests. Toxicology in Vitro 5, 535-538.
3. Bickers D. R. (1991) Xenobiotic metabolism in skin. In Physiology, Biochemistry and Molecular Biology of the Skin. Edited by L. A. Goldsmith, pp. 1480-1501. Oxford University Press, New York.
4. Boukamp P., Petrussevska R. T., Breitkreutz D., Hornung J., Markham A. and Fusenig N. E. (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. Journal of Cell Biology 106, 761-771.
5. Boyce S. T. and Ham R. G. (1985) Cultivation, frozen storage and clonal growth of normal human epidermal keratinocytes in serum-free media. Journal of Tissue Culture Methods 9, 83-92.
6. Braut-Boucher F., Pichon J., Rat P., Adolphe M., Aubery M. and Font J. (1995) A non isotopic highly sensitive fluorimetric cell-cell adhesion microplate assay using calcein AM-labelled lymphocytes. Journal of Immunological Methods 178, 41-51.
7. Daley J. P., Epstein D. A. and Hawley-Nelson P. (1990) Growth of human epidermal keratinocytes. Focus 12, 68.
8. Dickson F. M., Lawrence J. N. and Benford D. J. (1993) Release of inflammatory mediators in human keratinocyte cultures following exposure to a skin irritant. Toxicology in Vitro 7, 385-388.
9. Font J., Braut-Boucher F., Pichon J., Noël-Hudson M.-S., Bonnet M., Wepierre J. and Aubery M. (1994) New tridimensional culture of human keratinocytes - optimisation of the differentiation. Cell Biology and Toxicology 10, 353-359.
10. Fuchs E. (1990) Epidermal differentiation: the bare essentials. Journal of Cell Biology 111, 2807-2814.
11. Goegan P., Johnson G. and Vincent R. (1995) Effects of serum protein and colloid on the AlamarBlue assay in cell cultures. Toxicology in Vitro 9, 257-266.
12. Griffiths C. E. M., Esman J., Fisher G. J., Voorhees J. J. and Nickoloff B. J. (1990) Differential modulation of keratinocyte intercellular adhesion molecule-1 expression by gamma interferon and phorbol ester: evidence for involvement of protein kinase C. British Journal of Dermatology 122, 333-342.
13. Hedrick L., Cho K. R. and Vogelstein B. (1993) Cell adhesion molecules as tumor suppressors. Trends in Cell Biology 3, 36-39.
14. Hynes R. O. (1992) Integrins: Versatility, modulation and signaling in cell adhesion. Cell 69, 11-25.
15. Leigh I. M. and Watt F. M. (1994a) The culture of human epidermal keratinocytes. In The Keratinocyte Handbook. Edited by I. M. Leigh, E. B. Lane and F. M. Watt. pp. 43-153. Cambridge University Press, Cambridge.
16. Leigh I. M. and Watt F. M. (1994b) Keratinocyte adhesion. In The Keratinocyte Handbook. Edited by I. M. Leigh, B. Lane and F. M. Watt. pp. 153-165. Cambridge University Press, Cambridge.
17. Noël-Hudson M.-S., Braut-Boucher F., Robert M., Pichon J., Font J., Aubery M. and Wepierre J. (1995a) Multiple end-point assays to assess UVA. Human Experimental Toxicology, in press.
18. Noël-Hudson M.-S., Dusser I., Collober I., Muriel M.-P., Bonté F., Meybeck A., Font J. and Wepierre J. (1995b) Human epidermis reconstructed on synthetic membrane: Influence of experimental conditions on terminal differentiation. In Vitro Cellular Developmental Biology 31A, 508-515.
19. Pasqualetto V., Néel D., Feugeas J.-P., Aubery M. and Derappe C. (1995) HL60 leukaemia cells chemically induced to differentiate retain some surface glycan features of undifferentiated cells not found in normal leukocytes. Glycobiology 5, 59-66.
20. Rat P., Hildeilfinger J.-C., Simoulin J.-P., Warnet J.-M. and Adolphe M. (1992) Cell injury and antidote evaluations with fluorimetric microtitration assays. Toxicology Letters S, 238-239.
21. Régnier M., Prunieras M. and Woodley D. (1981) Growth differentiation of adult human epidermal cells on dermal substrates. Frontiers in Matrix Biology 9, 4-35.
22. Roguet R., Cohen C., Dossou K. G. and Rougier A. (1994) Episkin: a reconstituted human epidermis for assessing in vitro the irritancy of topically applied compounds. Toxicology in Vitro 8, 283-291.
23. Shahan T. A., Siegel W. G., Kuschner W. G. and Lewis D. M. (1994) A sensitive new bioassay for tumor necrosis factor. Journal of Immunological Methods 175, 181-187.
24. Schulz J., Dettlaff S., Fritzsche U., Schiebel H., Derer W., Fusenig N. E., Hülsen A. and Böhm M. (1994) The Amido-Black assay: a simple and quantitative multipurpose test of adhesion, proliferation, and cytotoxicity in microplate cultures of keratinocytes (HaCaT) and other cell types growing adherently or in suspension. Journal of Immunological Methods 167, 1-13.
25. Springer T. A. (1990) Adhesion receptors of the immune system. Nature 346, 425-433.
26. Virag L., Kerekgyarto C. and Fachet J. (1995) A simple, rapid and sensitive fluorimetric assay for the measurement of cell-mediated cytotoxicity. Journal of Immunological Methods 185, 199-208.
27. Watt F. M. and Hertle M. D. (1993) Keratinocyte integrins. In The Keratinocyte Handbook. Cambridge University Press, Cambridge.
28. Wilhelm K. P., Samblebe M. and Siegers C. P. (1994) Quantitative in vitro assessment of N-alkyl sulphate-induced cytotoxicity in human keratinocytes (HaCaT) - Comparison with in vivo human iritation tests. British Journal of Dermatology 130, 18-23.