Probing the structure of C1 with an anti-C1s monoclonal antibody: The possible existence of two forms of C1 in solution

Molecular Immunology

Volumn 34, Issue 8-9, 1997, Pages 671-679

  Tseng, Yiou ^a   Phillips, Martin L ^a   Schumaker, Verne N ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

C1; Clq; Clr; Cls; Generation of monoclonal antibody

Indexed keywords

COMPLEMENT COMPONENT C1; COMPLEMENT COMPONENT C1Q; COMPLEMENT COMPONENT C1R; COMPLEMENT COMPONENT C1S; MONOCLONAL ANTIBODY;

ARTICLE; CHEMICAL STRUCTURE; COMPLEMENT SYSTEM; COMPLEX FORMATION; HEMOLYSIS; HUMAN; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION;

ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; COMPLEMENT C1; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; HUMANS; MICE; MICE, INBRED BALB C; MICROSCOPY, ELECTRON; SOLUTIONS;

EID: 0030731019     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0161-5890(97)00039-4     Document Type: Article

References (16)

1. Arlaud G. J., Colomb M. G. and Gagnon J. (1987) A functional model of the human Cl complex. Immun. Today 8, 378-381.
2. Arlaud G. J., Sim R. B., Duplau A. M. and Colomb M. G. (1979) Differential elution of Clq, Clr and Cls from human Cl bound to immune aggregates; use in the rapid purification of Cl subcomponents. Molec. Immun. 16, 445-450.
3. Bianchino A. C., Poon P. H. and Schumaker V. N. (1988) A mechanism for the spontaneous activation of the first component of complement, Cl, and its regulation by Cl-inhibitor. J. Immun. 141, 3930-3936.
4. Colomb M. G., Arlaud G. J. and Villiers C. L. (1984) Activation of Cl. Philos. Trans. R. Soc. London, Ser. B 306, 283-292.
5. Lane P. D., Schumaker V. N., Tseng Y. and Poon P. H. (1991) Isolation of human complement subcomponent Clr and Cls in their unactivated, proenzyme forms. J. Immun. Methods 141,219-226.
6. Luo C., Thielens N. M., Gagnon I., Gal P., Sarvari M., Tseng Y., Tosi M., Zavodszky P., Arlaud G. T., Schumaker V. N. (1992) Recombinant human complement subcomponent Cls lacking β-hydroxyasparagine, sialic acid and one of its two carbohydrate chains still reassembles with Clq and Clr to form a functional Cl complex. Biochemistry 31, 4254-4262.
7. 2: within Cl. J. mokec. Biol. 179, 547-557.
8. Poon P. H., Schumaker V. N., Phillips M. L. and Strang C. J. (1983) Conformation and restricted segmental flexibility of Cl, the first component of human complement. J. molec. Biol. 168, 563-577.
9. Rapp H. J. and Borsos T. (1970) Molecular Basis of Complement Action, pp. 75-109, Appleton-Century-Crofts, New York.
10. Schumaker V. N. and Schachman H. K. (1957) Ultracentrifuge analysis of dilute solutions. Biochim. Biophys. Acta 23, 628-639.
11. Schumaker V. N., Zavodszky P. and Poon P. H. (1987) Activation of the first component of complement. A. Rev. Immun. 5, 21-42.
12. 2 tetramers. Molec. Immun. 20, 53-66.
13. Tseng Y., Zavodszky P. and Schumaker V. N. (1997) The human complement C1 complex has a picomolar dissociation constant at room temperature. J. Immun. 158, 937-944.
14. Tseng Y., Poon P. H., Zavodszky P. and Schumaker V. N. (1991) Spontaneous activation of serum Cl in vitro; the role of Cl-inhibitor. J. Immun. 147, 1884-1890.
15. Villiers C. L., Arlaud G. J. and Colomb M. G. (1985) Domain structure and associated functions of subcomponents Clr and Cls of the first component of human complement. Proc. nain. Acad. Sci. U.S.A. 82, 4477-4481.
16. Weiss V., Fauser C. and Engel J. (1986) Functional model of subcomponent Cl of human complement. J. molec. Biol. 189, 573-581.