Synthesis and turnover of embryonic sea urchin ciliary proteins during selective inhibition of tubulin synthesis and assembly

Molecular Biology of the Cell

Volumn 8, Issue 11, 1997, Pages 2187-2198

  Stephens, Raymond E ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; COLCHICINE; DYNEIN ADENOSINE TRIPHOSPHATASE; HEAT SHOCK PROTEIN 70; LEUCINE; PACLITAXEL; PROTEIN; TUBULIN;

ARTICLE; AUTOREGULATION; CONTROLLED STUDY; EMBRYO; FEEDBACK SYSTEM; GENE EXPRESSION; IMMUNOBLOTTING; MICROTUBULE ASSEMBLY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN METABOLISM; PROTEIN SYNTHESIS; PSEUDOPODIUM; REGENERATION; SEA URCHIN; STEADY STATE;

ECHINOIDEA; STRONGYLOCENTROTUS DROEBACHIENSIS;

EID: 0030730983     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.11.2187     Document Type: Article

References (37)

1. Anstrom, J.A. (1992). Organization of the ciliary basal apparatus in embryonic cells of the sea urchin, Lytechinus pictus. Tissue Cell Res. 269, 305-313.
2. Auclair, W., and Siegel, B.W. (1966). Cilia regeneration in the sea urchin embryo: evidence for a pool of ciliary proteins. Science 154, 913-915.
3. Bloch, M.A., and Johnson, K.A. (1995). Identification of a molecular chaperone in the eukaryotic flagellum and its localization to the site of microtubule assembly. J. Cell Sci. 108, 3541-3545.
4. Dunn, S.D. (1986). Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on western blots by monoclonal antibodies. Anal. Biochem. 157, 144-153.
5. Eldon, E.D., Angerer, L.M., Angerer, R.C., and Klein, W.H. (1987). Spec3: embryonic expression of a sea urchin gene whose product is involved in ectodermal ciliogenesis. Genes Dev. 1, 1280-1292.
6. Eldon, E.D., Montpetit, I.C., Nguyen, T., Decker, G., Valdizan, M.C., Klein, W.H., and Brandhorst, B.P. (1990). Localization of the sea urchin Spec3 protein to cilia and Golgi complexes of embryonic ectoderm cells. Genes Dev. 4, 111-122.
7. Fairbanks, G.T., Steck, T., and Wallach, D.F.H. (1971). Electrophoretic analysis of the major peptides of the human erythrocyte. Biochemistry 10, 2606-2617.
8. Gibbins, J.R., Tilney, L.G., and Porter, K.R. (1969). Microtubules in the formation and development of the primary mesenchyme in Arbacia punctulata. I. The distribution of microtubules. J. Cell Biol. 41, 201-226.
9. Gong, Z., and Brandhorst, B. (1988). Autogenous regulation of tubulin gene transcription via RNA stability during sea urchin embryogenesis. Development 102, 31-43.
10. Harlow, P., and Nemer, M. (1987). Developmental and tissue-specific regulation of β-tubulin gene expression in the embryo of the sea urchin Strongylocentrotus purpuratus. Genes Dev. 1, 147-160.
11. Haselgrove, J.C., Lyons, G., Rubenstein, N., and Kelly, A. (1985). A rapid, inexpensive, quantitative, general purpose densitometer and its application to one-dimensional gel electrophoretograms. Anal. Biochem. 150, 449-456.
12. Johnson, K.A., and Rosenbaum, J.L. (1992). Polarity of flagellar assembly in Chlamydomonas. J. Cell Biol. 72, 67-85.
13. Johnson, K.A., and Rosenbaum, J.L. (1993). Flagellar regeneration in Chlamydomonas: a model system for studying organelle assembly. Trends Cell Biol. 3, 156-161.
14. Kozminski, K.G., Beech, P.L., and Rosenbaum, J.L. (1995). The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane. J. Cell Biol. 131, 1517-1527.
15. Kreis, T.E., Geiger, B., and Schlessinger, J. (1982). Mobility of micro-injected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery. Cell 29, 835-845.
16. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
17. 14C in polyacrylamide gels by fluorography. Eur. J. Biochem. 56, 335-341.
18. Nelsen, E.M. (1975). Regulation of tubulin during ciliary regeneration in non-growing Tetrahymena. Exp. Cell Res. 94, 152-158.
19. Nojima, D., Linck, R.W., and Egelman, E.H. (1995). At least one of the protofilaments in flagellar microrubules is not composed of tubulin. Curr. Biol. 5, 158-167.
20. Norrander, J.M., Linck, R.W., and Stephens, R.E. (1995). Transcriptional control of tektin A mRNA correlates with cilia development and length determination during sea urchin embryogenesis. Development 121, 1615-1623.
21. FLA10 to reach the distal part of flagella in Chlamydomonas. J. Cell Biol. 133, 371-379.
22. Rosenbaum, J.L., and Child, F.M. (1967). Flagellar regeneration in protozoan flagellates. J. Cell Biol. 34, 345-364.
23. Saad, A.D., Dennis, J.E., Tan, I.P., and Fischman, D.A. (1991). Visualization of myosin exchange between synthetic thick filaments. J. Muscle Res. Cell Motil. 12, 225-234.
24. Stephens, R.E. (1977). Differential protein synthesis and utilization during cilia formation in sea urchin embryos. Dev. Biol. 61, 311-329.
25. Stephens, R.E. (1986). Isolation of embryonic cilia and sperm flagella. Methods Cell Biol. 27, 217-227.
26. Stephens, R.E. (1989). Quantal tektin synthesis and ciliary length in sea urchin embryos. J. Cell Sci. 92, 403-413.
27. Stephens, R.E. (1991). Tubulin in sea urchin embryonic cilia: characterization of the membrane-periaxonemal matrix. J. Cell Sci. 100, 521-531.
28. Stephens, R.E. (1992). Tubulin in sea urchin embryonic cilia: post-translational modifications during regeneration. J. Cell Sci. 101, 837-845.
29. Stephens, R.E. (1994a). Tubulin and tektin in sea urchin embryonic cilia: pathways of protein incorporation during turnover and regeneration. J. Cell Sci. 107, 683-692.
30. Stephens, R.E. (1994b). Developmental regulation of ciliogenesis and ciliary length in sea urchin and surf clam embryos. In: Reproduction and Development of Marine Invertebrates, ed. W.H. Wilson, Jr., S.A. Stricker, and G.L. Shinn, Baltimore, MD: Johns Hopkins University Press, 129-139.
31. Stephens, R.E. (1995a). Ciliogenesis in sea urchin embryos - a subroutine in the program of development. BioEssays 17, 331-340.
32. Stephens, R.E. (1995b). Rapid protein turnover in embryonic sea urchin cilia continues during colchicine-inhibition of tubulin synthesis. Mol. Biol. Cell 6, 358a.
33. Stephens, R.E. (1996). Selective incorporation of architectural proteins into terminally-differentiated molluscan gill cilia. J. Exp. Zool. 274, 330-309.
34. Stephens, R.E., Oleszko, S., and Linck, R.W. (1989). Retention of ciliary ninefold structure after removal of microtubules. J. Cell Sci. 92, 391-402.
35. Stephens, R.E., and Prior, G. (1995). Dynein inner arm heavy chain identification in cAMP-activated flagella using class-specific polyclonal antibodies. Cell Motil. Cytoskeleton 30, 261-271.
36. Tilney, L.G., and Gibbins, J.R. (1968). Differential effects of antimitotic agents on the stability and behavior of cytoplasmic and ciliary microtubules. Protoplasma 65, 167-179.
37. Vikstrom, K.L., Lim, S.S., Goldman, R.D., and Borisy, G.G. (1992). Steady state dynamics of intermediate filament networks. J. Cell Biol. 118, 121-129.