Identification and characterization of the two-enzyme system catalyzing oxidation of EDTA in the EDTA-degrading bacterial strain DSM 9103

Journal of Bacteriology

Volumn 179, Issue 22, 1997, Pages 6937-6943

  Witschel, Margarete ^a   Nagel, Samuel ^a   Egli, Thomas ^a  

^a SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

EDETIC ACID;

ARTICLE; BACTERIUM ISOLATION; CATALYSIS; COMPLEX FORMATION; DEGRADATION; ENZYME ANALYSIS; ENZYME PURIFICATION; NONHUMAN; PHOTOLYSIS; PRIORITY JOURNAL; SEWAGE DISPOSAL; VIBRIONACEAE; WASTE WATER MANAGEMENT;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PHOTOBACTERIUM; VIBRIO FISCHERI; VIBRIONACEAE;

EID: 0030728465     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.22.6937-6943.1997     Document Type: Article

References (43)

1. Alder, A. C., H. Siegrist, W. Gujer, and W. Giger. 1990. Behaviour of NTA and EDTA in biological wastewater treatment. Water Res. 24:733-742.
2. Belly, R. T., J. J. Lauff, and C. T. Goodhue. 1975. Degradation of ethylenediaminetetraacetic acid by microbial populations from an aerated lagoon. Appl. Microbiol. 29:787-794.
3. Bolton, H., Jr. 1993. Biodegradation of synthetic chelates in subsurface sediments from the southeast coastal plain. J. Environ. Qual. 22:125-132.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Cripps, R. E., and A. S. Noble. 1973. The metabolism of nitrilotriacetate by a pseudomonad. Biochem. J. 136:1059-1068.
6. Dietz, F. 1987. Neue Messergebnisse über die Belastung von Trinkwasser mit EDTA. Gas-Wasser-Fach Wasser Abwasser 128:286-288.
7. Drechsel, H., J. Metzger, S. Freund, G. Jung, J. R. Boelaert, and G. Winkelmann. 1991. Rhizoferrin - a novel siderophore from the fungus Rhizopus microsporus var. rhizopodiformis. Biol. Metals 4:238-243.
8. Egli, T., H. U. Weilenmann, T. El-Banna, and G. Auling. 1988. Gramnegative, aerobic, nitrilotriacetate-utilizing bacteria from wastewater and soil. Syst. Appl. Microbiol. 10:297-305.
9. Firestone, M. K., and J. M. Tiedje. 1978. Pathway of degradation of nitrilotriacetate by a Pseudomonas species. Appl. Environ. Microbiol. 35:955-961.
10. Frimmel, F. H., R. Grenz, E. Kordik, and F. Dietz. 1989. Nitrilotriacetat (NTA) und Ethylendinitrilotetraacetat (EDTA) in Fließgewässern der Bundesrepublik Deutschland. Vom Wasser 72:175-184.
11. Ghisla, S., and V. Massey. 1989. Mechanisms of flavoprotein-catalyzed reactions. Eur. J. Biochem. 181:1-17.
12. Gschwind, N. 1992. Biologischer Abbau von EDTA in einem Modellabwasser. Gas-Wasser-Fach Wasser Abwasser 133:546-549.
13. Haag, H., H.-P. Fiedler, J. Meiwes, H. Drechsler, G. Jung, and H. Zähner. 1994. Isolation and biological characterization of staphyloferrin B, a compound with siderophore activity from staphylococci. FEMS Microbiol. Lett. 115:125-130.
14. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
15. Hastings, J. W., and K. H. Nealson. 1977. Bacterial bioluminescence. Annu. Rev. Microbiol. 31:549-595.
16. Houriet, J.-P. 1996. NTA dans les eaux. Cahier de l'environnement 264. Office Fédéral de l'Environnement, des Forêts et du Paysage, Bern, Switzerland.
17. Inouye, S. 1994. NAD(P)H-flavin oxidoreductase from the bioluminescent bacterium, Vibrio fischen ATCC 7744, is a flavoprotein. FEES Lett. 347:163-168.
18. Kari, F. G. 1994. Umweltverhalten von Ethylendiamintetraacetat (EDTA) unter spezieller Berücksichtigung des photochemischen Abbaus. Ph.D. thesis 10698. Swiss Federal Institute of Technology, Zürich, Switzerland.
19. Kari, F. G., and W. Giger. 1995. Modeling the photochemical degradation of ethylenediaminetetraacetate in the river Glatt. Environ. Sci. Technol. 1995: 2814-2827.
20. Kendrew, S. G., S. E. Harding, D. A. Hopwood, and E. N. G. Marsh. 1995. Identification of a flavin:NADH oxidoreductase involved in the biosynthesis of actinorhodin. J. Biol. Chem. 270:17339-17343.
21. Klüner, T. 1996. Chemie und Biochemie des mikrobiellen EDTA-Abbaus. Ph.D. thesis. Universität-Gesamthochschule Paderborn, Paderborn, Germany.
22. Knobel, H.-R., T. Egli, and J. R. van der Meer. 1996. Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600. J. Bacteriol. 178:6123-6132.
23. Konetschny-Rapp, S., G. Jung, J. Meiwes, and H. Zähner. 1990. Staphyloferrin A: a structurally new siderophore from staphylococci. FEBS Eur. J. Biochem. 191:65-74.
24. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
25. Lauff, J. J., D. B. Steele, L. A. Coogan, and J. M. Breitfeller. 1990. Degradation of the ferric chelate of EDTA by a pure culture of an Agrobacterium sp. Appl. Environ. Microbiol. 56:3346-3353.
26. Madsen, E. L., and M. Alexander. 1985. Effects of chemical speciation on the mineralization of organic compounds by microorganisms. Appl. Environ. Microbiol. 50:342-349.
27. Meiwes, J., H.-P. Fiedler, H. Haag, H. Zähner, S. Konetschny-Rapp, and G. Jung. 1990. Isolation and characterization of staphyloferrin A, a compound with siderophore activity from Staphylococcus hyicus DSM 20459. FEMS Microbiol. Lett. 67:201-206.
28. Nishikiori, T., A. Okuyama, T. Naganawa, T. Takita, M. Hamada, T. Takeuchi, T. Aoyagi, and H. Umezawa. 1984. Production by actinomycetes of (S,S)-N,N′-ethylenediamine-disuccinic acid, an inhibitor of phospholipase C. J. Antibiot. 37:426-427.
29. Nörtemann, B. 1992. Total degradation of EDTA by mixed cultures and a bacterial isolate. Appl. Environ. Microbiol. 58:671-676.
30. Nowack, B. 1996. Behaviour of EDTA in groundwater - a study of surface reactions of metal-EDTA complexes. Ph.D. thesis 11392. Swiss Federal Institute of Technology, Zürich, Switzerland.
31. Nusch, E. A., H. D. Eschke, and K. H. Kornatzki. 1991. Die Entwicklung der NTA- und EDTA-Konzentrationen im Ruhrwasser und daraus gewonnenem Trinkwasser. Korrespondenz Abwasser 38:944-949.
32. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
33. Smith, A. W., J. N. Shoolery, B. Scbwyn, I. Holden, and J. B. Neilands. 1985. Rhizobactin, a structurally novel siderophore from Rhizobium meliloti. J. Am. Chem. Soc. 107:1739-1743.
34. A biosynthesis. J. Bacteriol. 177:5199-5205.
35. Tiedje, J. M. 1975. Microbial degradation of ethylenediaminetetraacetate in soils and sediments. Appl. Microbiol. 30:327-329.
36. Trijbels, F., and G. D. Vogels. 1966. Degradation of allantoin by Pseudomonas acidovorans. Biochim. Biophys. Acta 113:292-301.
37. Uetz, T., and T. Egli. 1993. Characterization of an inducible membranebound iminodiacetate dehydrogenase from Chelatobacter heintzii ATCC 29600. Biodegradation 3:423-434.
38. Uetz, T., R. Schneider, M. Snozzi, and T. Egli. 1992. Purification and characterization of a two-component monooxygenase that hydroxylates nitrilotriacetate from "Chelatobacter" strain ATCC 29600. J. Bacteriol. 174:1179-1188.
39. Wilkinson, G. (ed.). 1987. Comprehensive coordination chemistry. Pergamon Press, Oxford, England.
40. Witschel, M., H.-U. Weilenmann, and T. Egli. 1995. Degradation of EDTA by a bacterial isolate. Poster presented at the 54th Annual Meeting of the Swiss Society of Microbiology.
41. Wolf, K., and P. A. Gilbert. 1992. EDTA - ethylenediaminetetraacetic acid, p. 243-259. In O. Hutzinger (ed.), The handbook of environmental chemistry, vol. 3. Springer Verlag KG, Berlin, Germany.
42. Xi, L., C. H. Squires, D. J. Monticello, and J. D. Childs. 1997. A flavin reductase stimulates DszA and DszC proteins of Rhodococcus erythropolis IGTS8 in vitro. Biochem. Biophys. Res. Commun. 230:73-75.
43. Xu, Y., M. W. Mortimer, T. S. Fisher, M. L. Kahn, F. J. Brockman, and L. Xun. 1997. Cloning, sequencing, and analysis of a gene cluster from Chelatobacter heintzii ATCC 29600 encoding nitrilotriacetate monooxygenase and NADH:flavin mononucleotide oxidoreductase. J. Bacteriol. 179:1112-1116.