Isolation and preparation of single mouse cardiomyocytes for confocal microscopy

Methods in Cell Science

Volumn 19, Issue 2, 1997, Pages 83-90

  Cambon, Natalie ^a   Sussman, Mark A ^a  

^a CINCINNATI CHILDREN S HOSPITAL RESEARCH FOUNDATION   (United States)

Author keywords

Cardiomyocyte; Confocal microscopy; Myofibril; Transgenic mice

Indexed keywords

POLYESTER;

ANIMAL CELL; ARTICLE; CELL DEGENERATION; CELL ISOLATION; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; HEART MUSCLE CELL; MOUSE; MUSCLE FIBRIL; NONHUMAN;

EID: 0030726270     PISSN: 13815741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1009738012742     Document Type: Article

References (16)

1. Cavanaught MW (1995). Growth of chick heart cells in monolayer culture. J Exp Zool 128: 573-581.
2. Moscona A, Moscona H (1952). The dissociation and aggregation of cells from organ rudiments of the early chick embryo. J Anat 86: 287-301.
3. Bkaily G, Sperelakis N, Doane J (1984). A new method for preparation of isolated single adult myocytes. Am J Physiol 247: H1018-H1026.
4. Hein S, Scheffold T, Schaper J (1995). Ischemia indices early changes to cytoskeletal and contractile proteins in diseased human myocardium. J Thoracic Cardiovasc Surg 110: 89-98.
5. Perriard JC, von Arx P, Bantle S, Eppenberger HM, Eppenberger-Eberhardt M, Messerli M, Soldati T (1992). Molecular analysis of protein sorting during the biogenesis of muscle cytoarchitecture. Soc Exp Biol Symp 46: 219-235.
6. Palermo J, Gulick J, Colbert M, Fewell J, Robbins J (1996). Transgenic remodeling of the contractile apparatus in the mammalian heart. Circ Res 78: 504-509.
7. Muthuchamy M, Grupp IL, Grupp G, O'Toole BA, Kier AB, Boivin GP, Neumann J, Wieczorek DF (1995). Molecular and physiological effects of overexpressing striated muscle β-tropomyosin in the adult murine heart. J Biol Chem 270: 30593-30603.
8. Milano CA, Dolber PC, Rockman HA, Bond RA, Venable ME, Allen LF, Lefkowitz RA (1994). Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy. Proc Nat Acad Sci USA 91: 10109-10113.
9. Luo W, Grupp IL, Harrer J, Ponniah S, Grupp G, Duffy JJ, Doetschman T, Kranias EG (1994). Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of beta-agonist stimulation. Circ Res 75: 401-409.
10. Milner DJ, Weitzer G, Tran D, Bradley A, Capetanaki Y (1996). Disruption of muscle architecture and myocardial degeneration in mice lacking desmin. J Cell Biol 134: 1255-1270.
11. Argao EA, Kern MJ, Branford WW, Scott WJ Jr and Potter SS (1995). Malformations of the heart, kidney, palate, and skeleton in alpha-MHC-Hoxb-7 transgenic mice. Mech Dev 52: 291-303.
12. Katz EB, Steinhelper ME, Delcarpio JB, Daud AI, Claycomb WC, Field LJ (1992). Cardiomyocyte proliferation in mice expressing alpha-cardiac myosin heavy chain-SV40 T-antigen transgenes. Am J Physiol 262: H1867-1876.
13. Koch WJ, Rockman HA, Samama P, Hamilton RA, Bond RA, Milano CA, Lefkowitz RJ (1995). Cardiac function in mice overexpressing the beta-adrenergic receptor kinase or a beta ARK inhibitor. Science 268: 1350-1353.
14. Messerli JM, Perriard J-C (1995). Three-dimensional analysis and visualization of myofibrillogenesis in adult cardiomyocytes by confocal microscopy. Micros Res and Tech 30: 521-530.
15. Masson-Pevet M, Jongsma HJ, De Bruijne J (1976). Collagenase- and trypsin-dissociated heart cells: a comparative ultrastructural study. J Mol Cell Cardiol 8: 747-757.
16. Rindt H, Subramanaim A, Robbins J (1995). An in vivo analysis of transcriptional elements in the mouse α-myosin heavy chain gene promoter. Transgen Res 4: 397-405.