Gene amplification (PCR) to detect and differentiate mycoplasmas in porcine mycoplasmal pneumonia

Letters in Applied Microbiology

Volumn 25, Issue 5, 1997, Pages 327-330

  Stemke, G W ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GENE EXPRESSION;

CAUSATIVE AGENTS; CROSS REACTION; GENE AMPLIFICATION; LUNG TISSUE; MYCOPLASMA HYOPNEUMONIAE; MYCOPLASMA SPECIES; NESTED PCR; PCR ASSAY; PCR DETECTION; SEROLOGICAL IDENTIFICATION;

POLYMERASE CHAIN REACTION;

ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIUM IDENTIFICATION; GENE AMPLIFICATION; LUNG; MYCOPLASMA; MYCOPLASMA PNEUMONIA; NONHUMAN; POLYMERASE CHAIN REACTION; SWINE;

ANIMALIA; BACTERIA (MICROORGANISMS); MYCOPLASMA; MYCOPLASMA HYOPNEUMONIAE; MYCOPLASMATALES; SUIDAE; SUS SCROFA;

EID: 0030723572     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1997.00243.x     Document Type: Article

References (17)

1. Armstrong, C.H., Freeman, M.J., Sands-Freeman, L., Lopez-Osuna, M., Young, T. and Runnels, L.J. (1983) Comparison of the enzyme-linked immunosorbent assay and the indirect haemagglutination and complement fixation tests for detecting antibody to Mycoplasma hyopneumoniae. Canadian Journal of Comparative Medicine 47, 464-470.
2. Armstrong, C.H., Scheidt, A.B., Thacker, H.L., Runnels, L.J. and Freeman, M.J. (1984) Evaluation of criteria for the postmortem diagnosis of mycoplasmal pneumonia of swine. Canadian Journal of Comparative Medicine 48, 278-281.
3. Artiushin, S., Stipkovits, L. and Minion, F.C. (1993) Development of polymerase chain reaction primers to detect Mycoplasma hyopneumoniae, Molecular and Cellular Probes 7, 381-385.
4. Bereiter, M., Young, T.F., Joo, H.S. and Ross, R.R. (1990) Evaluation of the ELISA and comparison to the complement fixation test and radial immunodiffusion enzyme assay for detection of antibodies against Mycoplasma hyopneumoniae in swine serum. Veterinary Microbiology 25, 177-192.
5. Deng, S., Huruki, C., Robertson, J.A. and Stemke, G.W. (1991) Detection by PCR and differentiation by restriction fragment length polymophism of Acholeplasma, Spiroplasma, Mycoplasma, and Ureaplusma, based upon 16S rRNA genes. PCR Methods and Applications 1, 202-204.
6. Friis, N.F. (1972) Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare. Nordisk Veterinaermedicin 27, 337-339.
7. Friis, N.F. (1973) The pathogenicity of Mycoplasma flocculare. Acta Veterinaric Scandinavica. 14, 344-346.
8. Harasawa, R., Koshmizu, K., Takeda, O., Takashi, U., Asada, K. and Kato, I. (1991) Detection of Mycoplasma hyopneumoniae DNA by the polymerase chain reaction. Molecular and Cellular Probes 5, 103-109.
9. Lench, N., Stanier, P. and Williamson, R. (1988) Simple non-invasive method to obtain DNA for gene analysis. Lancet i, 1356-1358.
10. Mattson, J.G., Bergstrom, K., Wallgren, P. and Johansson, K.-E. (1995) Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene. Journal of Clinical Microbiology 33, 893-897.
11. Rose, D.L., Tully, J.T. and Wittier, R.G. (1979) Taxonomy of some swine mycoplasmas; Mycoplusma suipneumoniae Goodwin et al. 1965, a later objective synonym of Mycoplasma hyopneumoniae Mare and Switzer 1965, and the status of Mycoplasma flocculare Meyling and Friis 1972. International Journal of Systematic Bacteriology 29, 83-91.
12. Ross, R.R. (1981) Mycoplasmal diseases. In Diseases of Swine, 5th edn. ed. Leman, A.D., Glock, R.D., Mengeling, W.L., Penny, R.H.C., Scholl, E. and Straw, B. pp. 535-549. Ames, Iowa: Iowa State University Press.
13. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning, a Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
14. Stemke, G.W., Laigret, F., Grau, O. and Bove, J.M. (1992) Phylogenetic relationships of three porcine mycoplasmas, Mycoplasma hyopneumoniae, Mycoplusma flocculare, and Mycoplasma hyorhinis, and complete 16S rRNA sequence of M. flocculare. International Journal of Systematic Bacteriology 42, 220-225.
15. Stemke, G.W., McIntyre, D.J., Roy, K.L., Stemler, M.E. and Robertson, J.A. (1985) Guanine-plus-cytosine compositions of and deoxyribonucleic acid hybridizations between Mycoplasma hyopneumoniae and Mycoplasma flocculare. International Journal of Systematic Bacteriology 35, 527-529.
16. Stemke, G.W., Phan, R., Young, T.F. and Ross, R.R. (1994) Differentiation of Mycoplasma hyopneumoniae, M. flocculare, and M. hyorhinis on the basis of amplification of a 16S rRNA gene sequence. American Journal of Veterinary Research 55, 81-84
17. Zimmermann, K., Pischinger, K. and Mannhalter, J.W. (1993) Non-radioactive detection of HIV-1 RNA from a single-cell equivalent by reverse transcription PCR with nested primers. BioTechniques 15, 807-808.