Mutations in the hrp48 gene, which encodes a Drosophila heterogeneous nuclear ribonucleoprotein particle protein, cause lethality and developmental defects and affect P-element third-intron splicing in vivo

Molecular and Cellular Biology

Volumn 17, Issue 12, 1997, Pages 7260-7267

  Hammond, Linda E ^a   Rudner, David Z ^a   Kanaar, Roland ^b   Rio, Donald C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN HRP 48; UNCLASSIFIED DRUG;

ARTICLE; DROSOPHILA; EMBRYOPATHY; FEMALE; GENE MUTATION; INTRON; LETHAL MUTANT; MALE; NONHUMAN; PRIORITY JOURNAL; RNA SPLICING;

DROSOPHILA MELANOGASTER; MELANOGASTER;

EID: 0030723110     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.17.12.7260     Document Type: Article

References (40)

1. Adams, M. D., R. S. Tarng, and D. C. Rio. 1997. The alternative splicing factor PSI regulates P-element third intron splicing in vivo. Genes Dev. 11:129-138.
2. Ashburner, M. 1989. Drosophila: a laboratory handbook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
3. Ashburner, M. 1989. Drosophila: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
4. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1987. Current protocols in molecular biology. Wiley and Sons, New York, N.Y.
5. Burd, C. G., and G. Dreyfuss. 1994. Conserved structures and diversity of functions of RNA-binding proteins. Science 265:615-621.
6. Burd, C. G., and G. Dreyfuss. 1994. RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13:1197-1204.
7. Cáceres, J. F., S. Stamm, D. M. Helfman, and A. R. Krainer. 1994. Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science 265:1706-1709.
8. Chain, A. C., S. Zollman, J. C. Tseng, and F. A. Laski. 1991. Identification of a cis-acting sequence required for germ line-specific splicing of the P element ORF2-ORF3 intron. Mol. Cell. Biol. 11:1538-1546.
9. Choi, Y. D., P. J. Grabowski, P. A. Sharp, and G. Dreyfuss. 1986. Heterogeneous nuclear ribonucleoproteins: role in RNA splicing. Science 231: 1534-1539.
10. Dreyfuss, G., M. J. Matunis, S. Pinol-Roma, and C. G. Burd. 1993. hnRNP proteins and the biogenesis of mRNA. Annu. Rev. Biochem. 62:289-321.
11. Hammond, L. E., D. Z. Rudner, and D. C. Rio. Unpublished data.
12. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
13. Haynes, S. Personal communication.
14. Kraus, M. E., and J. T. Lis. 1994. The concentration of B52, an essential splicing factor and regulator of splice site choice in vitro, is critical for Drosophila development. Mol. Cell. Biol. 14:5360-5370.
15. Laski, F. A., D. C. Rio, and G. M. Rubin. 1986. Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing. Cell 4:7-19.
16. Laski, F. A., and G. M. Rubin. 1989. Analysis of cis-acting requirements for germline-specific splicing of the P element ORF2-ORF3 intron. Genes Dev. 3:720-728.
17. Mancebo, R., and S. Mount. Personal communication.
18. Matunis, E. L., R. Kelley, and G. Dreyfuss. 1994. Essential role for a heterogeneous nuclear ribonucleoprotein (hnRNP) in oogenesis: hrp40 is absent from the germ line in the dorsoventral mutant squid. Proc. Natl. Acad. Sci. USA 91:2781-2784.
19. Matunis, E. L., M. J. Matunis, and G. Dreyfuss. 1993. Association of individual hnRNP proteins and snRNPs with nascent transcripts. J. Cell Biol. 121:219-228.
20. Matunis, E. L., M. J. Matunis, and G. Dreyfuss. 1992. Characterization of the major hnRNP proteins from Drosophila melanogaster. J. Cell Biol. 116: 257-269.
21. Matunis, E. L., M. J. Matunis, and G. Dreyfuss. 1992. Isolation of hnRNP complexes from Drosophila melanogaster. J. Cell Biol. 116:245-255.
22. Mayeda, A., and A. R. Krainer. 1992. Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2. Cell 68:365-375.
23. Mayeda, A., S. Munroe, J. F. Cáceres, and A. R. Krainer. 1994. Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins. EMBO J. 13:5483-5495.
24. Mayrand, S. H., and T. Pederson. 1990. Crosslinking of hnRNP proteins to pre-mRNA requires U1 and U2 snRNPs. Nucleic Acids Res. 18:3307-3318.
25. Misra, S., and D. C. Rio. 1990. Cytotype control of Drosophila P element transposition: the 66kD protein is a repressor of transposase activity. Cell 62:269-284.
26. Pinol-Roma, S., and G. Dreyfuss. 1992. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature 355:730-732.
27. Pontius, B. W. 1993. Close encounters: why unstructured, polymeric domains can increase rates of specific macromolecular association. Trends Biochem. Sci. 18:181-186.
28. Ring, H. Z., and J. T. Lis. 1994. The SR protein B52/SRp55 is essential for Drosophila development. Mol. Cell. Biol. 14:7499-7506.
29. Roche, S. E., M. Schiff, and D. C. Rio. 1995. P-element repressor autoregulation involves germ-line transcriptional repression and reduction of third intron splicing. Genes Dev. 9:1278-1288.
30. Rudner D. Z., K. Breger, and D. C. Rio. Unpublished data.
31. Rudner D. Z., R. Kanaar, K. S. Breger, and D. C. Rio. 1996. Mutations in the small subunit of the Drosophila U2AF splicing factor causes lethality and developmental defects. Proc. Natl. Acad. Sci. USA 93:10333-10337.
32. Shen, J., K. Zu, C. L. Cass, A. L. Beyer, and J. Hirsh. 1995. Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo. Proc. Natl. Acad. Sci. USA 92:1822-1825.
33. Siebel, C. W., A. Admon, and D. C. Rio. 1995. Soma-specific expression and cloning of PSI, a negative regulator of P element pre-mRNA splicing. Genes Dev. 9:269-283.
34. Siebel, C. W., L. D. Fresco, and D. C. Rio. 1992. The mechanism of somatic inhibition of Drosophila P-element pre-mRNA splicing: multiprotein complexes at an exon pseudo-5′ splice site control U1 snRNP binding. Genes Dev. 6:1386-1401.
35. Siebel, C. W., R. Kanaar, and D. C. Rio. 1994. Regulation of tissue-specific P-element pre-mRNA splicing requires the RNA-binding protein PSI. Genes Dev. 8:1713-1725.
36. Siebel, C. W., and D. C. Rio. 1990. Regulated splicing of the Drosophila P transposable element third intron in vitro: somatic repression. Science 248: 1200-1208.
37. Spradlinz, A. C. 1986. P element-mediated transformation, p. 175-197. In D. Roberts (ed.), Drosophila: a practical approach. IRL Press, Washington, D.C.
38. Spradling, A. C., and G. M. Rubin. 1982. Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218:341-347.
39. B proteins of heterogeneous nuclear ribonucleoparticles modulate 5′ splice site selection in vivo. Proc. Natl. Acad. Sci. USA 91:6924-6928.
40. Zu, K., M. L. Sikes, S. R. Haynes, and A. L. Beyer. 1996. Altered levels of the Drosophila HRB87F/hrp36 hnRNP protein have limited effects on alternative splicing in vivo. Mol. Biol. Cell 7:1059-1073.