Identification of pathogenic Leptospira genospecies by continuous monitoring of fluorogenic hybridization probes during rapid-cycle PCR

Journal of Clinical Microbiology

Volumn 35, Issue 12, 1997, Pages 3140-3146

  Woo, Tony H S ^a   Patel, B K C ^a   Smythe, Lee D ^b   Symonds, Meegan L ^b   Norris, Michelle A ^b   Dohnt, Michael F ^b  

^a GRIFFITH UNIVERSITY   (Australia)

^b WHO COLLABORATING CENTRE FOR REFERENCE AND RESEARCH ON INFLUENZA   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; RNA 23S;

ARTICLE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; FLUORESCENCE; LEPTONEMA ILLINI; LEPTOSPIRA; LEPTOSPIRA BIFLEXA; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPIROCHETE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LEPTONEMA ILLINI; LEPTOSPIRA; LEPTOSPIRA BIFLEXA; SPIROCHAETALES;

EID: 0030716762     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.12.3140-3146.1997     Document Type: Article

References (26)

1. Bonfield, J. K., K. F. Smith, and R. A. Staden. 1995. New DNA sequence assembly program. Nucleic Acids Res. 23:4992-4999.
2. Boom, R., C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E. Wertheimvan Dillen, and J. van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503.
3. Breslauer, K. J., R. Frank, H. Blocker, and L. A. Marky. 1986. Predicting DNA duplex stability from the base sequence. Proc. Natl. Acad. Sci. USA 83:3746-3750.
4. Cardullo, R. A., S. Agrawal, C. Flores, P. C. Zamecnik, and D. E. Wolf. 1988. Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. Proc. Natl. Acad. Sci. USA 85:8790-8794.
5. Corney, B. G., J. Colley, S. P. Djordjevic, R. Whittington, and G. C. Graham. 1993. Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting. J. Clin. Microbiol. 31:2927-2932.
6. De Caballero, O. L. S. D., E. D. Neto, M. C. Koury, A. J. Romanha, and A. J. G. Simpson. 1994. Low-stringency PCR with diagnostically useful primers for identification of Leptospira serovars. J. Clin. Microbiol. 32:1369-1372.
7. Ellinghausen, H. C., and W. G. McCullough. 1965. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am. J. Vet. Res. 26:45-51.
8. Fukunaga, M., T. Masuzawa, N. Okuzako, I. Mifuchi, and Y. Yanagihara. 1990. Linkage of ribosomal RNA genes in Leptospira. Microbiol. Immunol. 34:565-573.
9. Genetics Computer Group. 1994. Program manual for the Wisconsin Package, version 8. Genetics Computer Group, Madison, Wis.
10. Gerritsen, M. A., M. A. Smits, and T. Olyhoek. 1995. Random amplified polymorphic DNA fingerprinting for rapid identification of leptospiras of serogroup Sejroe. J. Med. Microbiol. 42:336-339.
11. Gerritsen, M. A., T. Olyhoek, M. A. Smits, and B. A. Bokhout. 1991. Sample preparation method for polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo subtype hardjobovis in bovine urine. J. Clin. Microbiol. 29:2805-2808.
12. Gravekamp, C., H. Van de Kemp, M. Franzen, D. Carrington, G. J. Schoone, G. J. J. M. Van Eys, C. O. R. Everard, R. A. Hartskeerl, and W. J. Terpstra. 1993. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J. Gen. Microbiol. 139:1691-1700.
13. Hillier, L., and P. Green. 1991. OSP: A computer program for choosing PCR and DNA sequencing primers. PCR Methods Applic. 1:124-128.
14. Hookey, J. V. 1992. Detection of Leptospiraceae by amplification of 16S ribosomal DNA. FEMS Microbiol. Lett. 90:267-274.
15. Kee, S., I. Kim, M. Choi, and W. Chang. 1994. Detection of leptospiral DNA by PCR. J. Clin. Microbiol. 32:1035-1039.
16. Merien, F., P. Amouriaux, P. Perolat, G. Baranton, and I. Saint Girons. 1992. Polymerase chain reaction for detection of Leptospira spp. in clinical samples. J. Clin. Microbiol. 30:2219-2224.
17. Oliveira, M. A. A., O. L. Caballero, E. D. Neto, M. C. Koury, A. J. Romanha, J. Carvalho, R. A. Hartskeerl, and A. J. G. Simpson. 1995. Use of nondenaturing silver-stained polyacrylamide gel analysis of polymerasc chain reaction amplification products for the differential diagnosis of Leptospira interrogans infection. Diagn. Microbiol. Infect. Dis. 22:343-348.
18. Pearson, W. R., and D. J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444-2448.
19. Savio, M. L., C. Rossi, P. Fusi, S. Tagliabue, and M. L. Pacciarini. 1994. Detection and identification of Leptospira interrogans scrovars by PCR coupled with restriction endonuclease analysis of amplified DNA. J. Clin. Microbiol. 32:935-941.
20. Wagenaar, J. A., R. P. A. M. Segers, and B. A. M. Van der Zeijst. 1994. Rapid and specific detection of pathogenic Leptospira species by amplification of rihosomal sequences. Mol. Biotechnol. 2:1-14.
21. Wilson, K. 1987. Preparation of genomic DNA from bacteria, p. 2.4.1-2.4.5. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology. Greene Publishing Associates and Wiley-Intcrscience, New York, N.Y.
22. Wittwer, C. T., M. G. Herrmann, A. A. Moss, and R. P. Rasmussen. 1997. Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques 22:130-138.
23. Woo, T. H. S., L. D. Smythe, M. L. Symonds, M. A. Norris, M. F. Dohnt, and B. K. C. Patel. 1997. Rapid distinction between Leptospira interrogans and Leptospira biflexa by PCR amplification of 23S ribosomal DNA. FEMS Microbiol. Lett. 150:9-18.
24. Woodward, M. J., G. J. Sullivan, N. M. A. Palmer, J. C. Woolley, and J. S. Redstone. 1991. Development of a PCR test specific for Leptospira hardjo genotype bovis. Vet. Rec. 128:282-283.
25. Zhang, Y., S. Li, and B. Dai. 1993. Amplified 23S rRNA gene of 52 strains of Leptospira and detection of leptospiral DNA in 55 patients by PCR. Huaxi Yike Daxue Xuebau 24:262-267.
26. Zuerner, R. L., D. Alt, and C. A. Bolin. 1995. IS1533-based PCR assay for identification of Leptospira interrogans sensu lato serovars. J. Clin. Microbiol. 33:3284-3289.