Deuterium oxide (heavy water) accelerates actin assembly in vitro and changes microfilament distribution in cultured cells

European Journal of Cell Biology

Volumn 74, Issue 3, 1997, Pages 273-280

  Omori, Hiroshi ^a   Kuroda, Masaaki ^a   Naora, Hiroyuki ^a,b   Takeda, Hiroshi ^a   Nio, Yoshinori ^a   Otani, Hiroki ^a   Tamura, Katsuhiro ^a  

^a SHIMANE UNIVERSITY   (Japan)

^b Department of Biology Faculty of Science Shimane University ^*  (Japan)

Author keywords

Actin; Deuterium oxide; Microfilaments

Indexed keywords

ALPHA ACTININ; DEUTERIUM OXIDE; F ACTIN; G ACTIN;

ACTIN POLYMERIZATION; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL STRAIN 3T3; FLUORESCENCE; MICROFILAMENT; MOUSE; NONHUMAN; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALIA;

EID: 0030716457     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Albrecht-Buehler G.: The phagokinetic tracks of 3T3 cells. Cell 11, 359-404 (1977).
2. Albrecht-Buehler, G.: Daughter 3T3 cells: Are they mirror images of each other? J. Cell Biol. 72, 595-603 (1977).
3. Amato, P. A., E. R. Unanue, D. L. Taylor: Distribution of actin in spreading macrophages. A comparative study on living and fixed cells. J. Cell Biol. 96, 750-761 (1983).
4. Blake, M. I., H. I. Crespi, J. J. Katz: Study with deuterated drugs. J. Pharm. Sci. 64, 367-391 (1975).
5. Blikstadt, I., F. Markey, L. Carlsson, T. Persson, U. Lindberg: Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I. Cell 15, 935-943 (1978).
6. Houston, L. L., J. Odell, Y. C. Lee, R. H. Himes: Solvent isotope effects on microtubule polymerization and depolymerization. J. Mol. Biol. 87, 141-146 (1974).
7. Itoh, T. J., H. Sato: The effects of deuterium oxide (2H2O) on the polymerization of tubulin in vitro. Biochim. Biophys. Acta 800, 21-27 (1984).
8. Katz, J. J., H. L. Crespi, R. J. Hasterlik, J. F. Thomson, A. J. Finkel: Some observations on biological effects of deuterium, with special reference of effects on neoplastic processes. J. Natl. Cancer Inst. 18, 641-659 (1957).
9. Katz, J. J., H. L. Crespi, A. J. Finkel: Isotope effects in fully deuterated hexoses, proteins and nucleic acids. Pure Appl. Chem. 8, 471-481 (1964).
10. Keller, H., V. Niggli, A. Zimmermann: Diversity in motile response of human neutrophil granulocytes. In: W. H. Hörl, P. J. Schollmeyer (eds.): New Aspects of Human Polymorphonuclear Leukocytes. pp 23-37. Plenum Press. New York (1991).
11. Kouyama, T., K. Mihashi: Fluorimetry study of N-[1-Pyrenyl]iodoacetamide-labelled F-actin. Eur. J. Biochem. 114, 33-38 (1981).
12. Kuroda, M., Y. Kohira, M. Sasaki: Conformational change of skeletal muscle α-actinin induced by salt. Biochim. Biophys. Acta 1205, 97-104 (1994).
13. Lamprecht, J., D. Schroeter, N. Paweletz: Disorganization of mitosis in HeLa cells by deuterium oxide. Eur. J. Cell Biol. 50, 360-369 (1989).
14. Lamprecht, J., D. Schroeter, N. Paweletz: Mitosis arrested by deuterium oxide. Light microscopic, immunofluorescence and ultrastructural characterization. Eur. J. Cell Biol. 51, 303-312 (1990).
15. Lowry O. H., N. J. Rosebrough, A. L. Farr, R. J. Randall: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275 (1951).
16. MacLean-Fletcher, S. D., T. D. Pollard: Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors. J. Cell Biol. 85, 414-428 (1980).
17. Marsland, D., A. Zimmermann: Structural stabilization of the mitotic apparatus by heavy water, in the cleaving eggs of Arbacia punctulata. Exp. Cell Res. 38, 306-313 (1965).
18. Marsland, D., L. G. Tilney, M. Hirshfield: Stabilizing effect of D2O on the microtubular components and needle-like form of heliozoan axopods: A pressure-temperature analysis. J. Cell. Physiol. 77, 187-194 (1971).
19. Schroeter, D., J. Lamprecht, P. Eckhardt, G. Futterman, N. Paweletz: Deuterium oxide (heavy water) arrests the cell cycle of PtK2 cells during interphase. Eur. J. Cell Biol. 58, 365-370 (1992).
20. Spudich, J. A., S. Watt: The regulation of rabbit skeletal muscle contraction. J. Biol. Chem. 246, 4866-4871 (1971).
21. Ueda, T., Y. Kobatake: Quantitative analysis of changes in cell shape of Amoeba proteus during locomotion and upon responses to salt stimuli. Exp. Cell Res. 147, 466-471 (1983).
22. Uratani Y.: Polymerization of Salmonella flagellin in water and deuterium oxide media. J. Biochem. 75, 1143-1151 (1974).
23. Urey, H. C., F. G. Brickwedde, G. M. Murphy: The separation and properties of the isotopes of hydrogen. Science 39, 164-165 (1933).
24. Zimmermann, A., H.-U. Keller, H. Cottier: Heavy water (D2O)-induced shape changes, movements and F-actin redistribution in human neutrophil granulocytes. Eur. J. Cell Biol. 47, 320-326 (1988).