Imaging of subcellular structures by scanning ion microscopy and mass spectrometry. Advantage of cryofixation and freeze substitution procedure over chemical preparation

Biology of the Cell

Volumn 89, Issue 3, 1997, Pages 199-210

  Mony, Marie Cécile ^a   Larras Regard, Eliane ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

Chemical characterization; Chemical fixation; Cryofixation freeze substitution; SIMS microscopy; Subcellular structures

Indexed keywords

CYANIDE; PHOSPHORUS; SULFUR;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BASEMENT MEMBRANE; CELL NUCLEUS; CELL ORGANELLE; CELL STRUCTURE; CELL VACUOLE; CHEMICAL COMPOSITION; CONTRAST; CONTROLLED STUDY; FREEZE DRYING; HEART MUSCLE CELL; IMAGING; INTERMETHOD COMPARISON; KIDNEY PARENCHYMA; KIDNEY TUBULE CELL; LYSOSOME; MASS SPECTROMETRY; MICROSCOPY; MITOCHONDRION; MUSCLE FIBRIL; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0030713373     PISSN: 02484900     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0248-4900(97)80036-6     Document Type: Article

References (53)

1. Allenspach A (1993) Ultrastructure of early chick embryo tissues after high pressure freezing and freeze substitution. Microsc Res Technique 24, 369-384
2. Bittermann AG, Knoll G, Németh A and Plattner H (1992) Quantitative immuno-gold labelling and ultrastructural preservation after cryofixation (combined with different freeze-substitution and embedding protocols) and after chemical fixation and cryosectioning. Histochemistry 97, 421-429
3. Boyles J (1984) The use of primary amines to improve glutaraldehyde fixation. In: The Science of Biological Specimen Preparation (Revel JP, Barnard T and Haggis GH, eds), SEM Inc, AMF O'Hare, Chicago, 7-21
4. Chan FL, Inoue S and Leblond CP (1993) Cryofixation of basement membranes followed by freeze substitution or freeze drying demonstrates that they are composed of a tridimensional network of irregular cords. Anat Rec 235, 191-205
5. Dalen H, Lieberman M, LeFurgey A, Scheie P and Sommer JR (1992) Quick-freezing of cultured cardiac cells in situ with special attention to the mitochondrial ultrastructure. J Microsc 168, 259-273
6. Dudek RW and Boyne AF (1986) An excursion through the ultrastructural world of quick-frozen pancreatic islets. Am J Anat 175, 217-243
7. Edelmann L (1984) Subcellular distribution of potassium in striated muscles. Scanning Electron Microsc II, 875-888
8. Edelmann L (1991a) Freeze substitution and the preservation of diffusible ions. J Microsc 161, 217-228
9. Edelmann L (1991b) Adsorption staining of freeze-substituted and low temperature embedded frog skeletal muscle with cesium: a new method for the investigation of protein-ion interactions. Scanning Microscopy Sup 5, S75-S84
10. Guthe KF (1954) The effect of formaldehyde on the oxygen equilibrium of hemoglobin. J Gen Physiol 37, 775-780
11. Hippe-Sanwald S (1993) Impact of freeze substitution on biological electron microscopy. Microsc Res Technique 24, 400-422
12. Horowitz A, Giannasca PJ and Woodcock CL (1990) Ultrastructural preservation of nuclei and chromatin: improvement with low-temperature methods. J Microsc 157, 205-224
13. Hunziker EB, Herrmann W, Schenk RK, Mueller M and Moor H (1984) Cartilage ultrastructure after high pressure freezing, freeze substitution, and low temperature embedding. Chondrocyte ultrastructure. Implications for the theories of mineralization and vascular invasion. J Cell Biol 98, 267-276
14. Ingram FD and Ingram MJ (1984) Influences of freeze-drying and plastic embedding on electrolyte distributions. In: The Science of Biological Specimen Preparation (Revel JP, Barnard T and Haggis GH, eds), SEM Inc, AMF O'Hare, Chicago, 167-174
15. James-Kracke MR, Sloane BF, Shuman H, Karp R and Somlyo AP (1980) Electron probe analysis of cultured vascular smooth muscle. J Cell Physiol 103, 313-322
16. Jorgensen AO and McGuffee LJ (1987) Immunoelectron microscopic localization of sarcoplasmic reticulum proteins in cryofixed, freeze-dried and low temperature-embedded tissue. J Histochem Cytochem 35, 723-732
17. Keene DR and McDonald K (1993) The ultrastructure of the connective tissue matrix of skin and cartilage after high-pressure freezing and freeze-substitution. J Histochem Cytochem 41, 1141-1153
18. Larras-Regard E and Mony M-C (1995) Scanning ion images; analysis of pharmaceutical drugs at organelle levels. Int J Mass Spectrometry Ion Processes 143, 147-160
19. LeFurgey A, Bond M and Ingram P (1988) Frontiers in electron probe microanalysis: application to cell physiology. Ultramicroscopy 24, 185-220
20. LeFurgey A, Davilla SD, Kopf DA, Sommer JR and Ingram P (1992) Real-time quantitative elemental analysis and mapping: microchemical imaging in cell physiology. J Microsc 165, 191-223
21. Marshall AT, Schroen C and Condron RJ (1994) X-ray microanalysis of renal proximal tubules in cadmium-treated rats. J Submicrosc Cytol Pathol 26, 59-66
22. Mason JT and O'Leary TJ (1991) Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. J Histochem Cytochem 39, 225-229
23. Maupin-Szamier P and Pollard TD (1978) Actin filament destruction by osmium tetroxide. J Cell Biol 77, 837-852
24. Menco BPhM (1989) Electron-microscopic demonstration of olfactory-marker protein with protein G-gold in freeze-substituted, Lowicryl K11M-embedded rat olfactory-receptor cells. Cell Tissue Res 256, 275-281
25. Mony MC and Larras-Regard E (1988) Apport des méthodes de congélation ultra-rapide et de cryosubstitution des tissus biologiques en microscopie ionique analytique. CR Soc Biol 182, 458-465
26. Mony MC and Larras-Regard E (1991) Visualization of glycocalyx calcium by means of SIMS. In: Secondary Ion Mass Spectrometry (SIMS VIII) (Benninghoven A, Janssen KTF, Tümpner J and Werner HW, eds), John Wiley & Sons, Chichester, 681-684
27. Nassar R, Wallace NR, Taylor I and Sommer JR (1986) The quick-freezing of single intact skeletal muscle fibers at known time intervals following electrical stimulation. Scanning Electron Microsc I, 309-328
28. Nicolas G (1991) Advantages of fast-freeze fixation followed by freeze-substitution for the preservation of cell integrity. J Electron Microsc Technique 18, 395-405
29. Nicolas MT, Bassot JM and Nicolas G (1989) Immunogold labeling of luciferase in the luminous bacterium Vibrio harveyi after fast-freeze fixation and different freeze-substitution and embedding procedures. J Histochem Cytochem 37, 663-674
30. Norby A, Tvedt KE, Halgunset J and Haugen OA (1990) Intracellular penetration and accumulation of radiographic contrast media in the rat kidney. Scanning Microsc 4, 651-666
31. Padron R and Craig R (1989) Disorder induced in nonoverlap myosin cross-bridges by loss of adenosine triphosphate. Biophys J 56, 927-933
32. Penttila A, Kalimo H and Trump BF (1974) Influence of glutaraldehyde and/or osmium tetroxide on cell volume, ion content, mechanical stability, and membrane permeability of ehrlich ascites tumor cells. J Cell Biol 63, 197-214
33. Phillips GW and Bridgman PC (1991) Immunoelectron microscopy of acetylcholine receptors and 43 kDa protein after rapid freezing, freeze-substitution, and low-temperature embedding in Lowicryl K11M. J Histochem Cytochem 39, 625-634
34. Reale E and Luciano L (1993) Further observations on the morphological alterations of the glomerular capillary wall of the rat kidney caused by chemical and physical agents: standard procedures versus quick-freezing and freeze-substitution. Histochem J 25, 357-366
35. Riederer BM, Porchet R, Marugg RA and Binder LI (1993) Solubility of cytoskeletal proteins in immunohistochemistry and the influence of fixation. J Histochem Cytochem 41, 609-616
36. Sandoz D, Nicolas G and Laine MC, (1985a) Two mucous cell types revisited after quick-freezing and cryo substitution. Biol Cell 54, 79-88
37. Sandoz D, Laine MC and Nicolas G (1985b) Distribution of microtubules within the intestinal terminal web as revealed by quick-freezing and cryosubstitution. Eur J Cell Biol 39, 481-484
38. Shea SM (1971) Lanthanum staining of the surface coat of cells. J Cell Biol 51, 611-620
39. Somlyo AP, Somlyo AV and Shuman H (1979) Electron probe analysis of vascular smooth muscle. J Cell Biol 81, 316-335
40. 2+, and other ions in rapidly frozen cells. Methods Enzymol 172, 203-229
41. Sommer JR, Johnson EA, Wallace NR and Nassar R (1988) Cardiac muscle following quick-freezing: preservation of in vivo ultrastructure and geometry with special emphasis on intercellular clefts in the intact frog heart. J Mol Cell Cardiol 20, 285-302
42. Stelly N, Halpern S, Nicolas G, Fragu P and Adoutte A (1995) Direct visualization of a vast cortical calcium compartment in paramecium by secondary ion mass spectrometry (SIMS) microscopy: possible involvement in exocytosis. J Cell Sci 108, 1895-1909
43. Von Schack ML, Fakan S and Villiger W (1991) Some applications of cryosubsitution in ultrastructural studies of the cell nucleus. Biol Cell 72, 113-119
44. Von Schack ML, Fakan S (1993) The study of the cell nucleus using cryofixation and cryosubstitution. Micron 24, 507-519
45. Von Zglinicki T (1988) Monovalent ions are spatially bound within the sarcomere. Gen Physiol Biophys 7, 495-504
46. Von Zglinicki T, Uhrik B (1988) X-ray microanalysis with continuous specimen cooling: is it necessary? J Microsc 151, 43-47
47. Warley A (1986) Elemental concentrations in isolated rat thymocytes prepared for cryofixation in the presence of different media. J Microsc 144, 183-191
48. Weibull C, Christiansson A and Carlemalm E (1983) Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawii-cells for electron microscopy. J Microsc 129, 201-207
49. Weibull C, Villiger W and Carlemalm E (1984) Extraction of lipids during freeze-substitution of Acholeplasma laidlawii-cells for electron microscopy. J Microsc 134, 213-216
50. Wendt-Gallitelli MF and Wolburg H (1984) Rapid freezing, Cryosectioning, and X-ray microanalysis on cardiac muscle preparations in defined functional states. J Electron Microsc Technique 1, 151-174
51. Wendt-Gallitelli MF and Isenberg G (1989) X-ray microanalysis of single cardiac myocytes frozen under voltage-clamp conditions. Am J Physiol 256, H574-H583
52. Wendt-Gallitelli MF, Voigt T and Isenberg G (1993) Microheterogeneity of subsarcolemmal sodium gradients, electron probe microanalysis in guinea-pig ventricular myocytes. J Physiol 472, 33-44
53. Zierold K and Schäfer D (1988) Preparation of cultured and isolated cells for X-ray microanalysis. Scanning Microsc 2, 1775-1790