Aromatase cytochrome P450 transcripts are detected in fractured human bone but not in normal skeletal tissue

Bone

Volumn 21, Issue 5, 1997, Pages 433-440

  Lea, C K ^a   Ebrahim, H ^a   Tennant, S ^b   Flanagan, A M ^a  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

^b ST MARY S HOSPITAL   (United Kingdom)

Author keywords

Aromatase cytochrome P450; Bone formation; Bone resorption; Fracture neck of femur; Osteoporosis

Indexed keywords

AROMATASE; COMPLEMENTARY DNA; CYTOCHROME P450; INTERLEUKIN 6; MESSENGER RNA;

ARTICLE; BONE; BONE TISSUE; CELL PROLIFERATION; CONTROLLED STUDY; CORTICAL BONE; FEMUR NECK FRACTURE; FRACTURE; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LEUKOCYTE; NONHUMAN; OSSIFICATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TRABECULAR BONE;

ANIMALS; AROMATASE; ARTHROPLASTY, REPLACEMENT, HIP; BLOTTING, SOUTHERN; BONE MARROW; CELLS, CULTURED; DNA, COMPLEMENTARY; FEMORAL NECK FRACTURES; FEMUR; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; INTERLEUKIN-6; LEUKOCYTES; POLYMERASE CHAIN REACTION; RATS; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 0030712149     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S8756-3282(97)00168-3     Document Type: Article

References (40)

1. 3, PTH and glucocorticoids. Metab Bone Dis Rel Res 5:229-235: 1984.
2. Bernstein, L. M., Santen, R. J., and Santern. S. J., Three-component model of oestrogen formation and regulation of inlratumoural oestrogen pool in breast neoplasms. Med Hypoth 45:588-590; 1995.
3. Bruch. H. R., Wolf, E., Budde. R., Romalo, G., and Schweikert, H.-U. Androstenedione metabolism in cultured human osteoblast-like cells. J Clin Endocrinol Metab 75:101-105; 1992.
4. Bulun. S. E., Economos. K., Miller, D., and Simpson, E. R. CYPI9 (aromatase cytochrome P450) gene expression in human malignant endometrial tumors. J Clin Endocrinol Metab 79:1831-1834; 1994.
5. Bulun, S. E., Price, T. M., Aitken, J., Mahendroo, M. S., and Simpson, E. R. A link between breast cancer and local estrogen biosynthesis suggested by quantification of breast adipose tissue aromatase cytochrome P450 transcripts using competitive polymerase chain reaction after reverse transcription. J Clin Endocrinol Metab 77:1622-1628; 1993.
6. Bulun, S. E. and Simpson, E. R. Competitive reverse transcription-polymerase chain reaction analysis indicates that levels of aromatase cytochrome P450 transcripts in adipose tissue of buttocks, thighs and abdomen of women increases with advancing age. J Clin Endocrinol Metab 78:428-432: 1994.
7. Cauley, J. A., Gutai. J. P., Kuller, E. H., LeDonne, D., and Powell. J. G. The epidemiology of serum sex hormones in postmenopausal women. Am J Epidemiol 129:1120-1131: 1989.
8. Chavassieux. P. M., Chenu. C., Valentin-Opran, A., Merle. B., Delmas. P. D., Hartmann, D. J., Saez, S., and Meunier, P. J. Influence of experimental conditions on osteoblast activity in human primary bone cell cultures. J Bone Miner Res 5:337-343; 1990.
9. Christiansen, C., Christiansen, M. S., Mcnair, P., Hagen. C., Stocklund, K.-E., and Transbol, I. Prevention of early postmenopausal bone loss. Controlled 2-year study in 315 normal females. Eur J Clin Invest 10:273-279; 1980.
10. Deutsch. S., Benjamin. F., Seltzer, V., Tafreshi. M., Kocheril, G., and Frank. A. The correlation of serum estrogens and androgens with bone density in late menopause. Int J Gynecol Obstet 25:217-225: 1987.
11. Frisch, R. E., Canick. J. A., and Tulchinsky, D. Human fatty marrow aromatises androgens to estrogen. J Clin Endocrinol Metab 51:394-396; 1980.
12. Fuller, K., Owens, J. M., and Chambers. T. J. Macrophage inflammatory protein (MIP-lα) and interleukin 8 (IL-8) stimulate the motility but suppress the resorption of isolated rat osteoclasts. J Immunol 54:6065-6072; 1995.
13. Genant, H. K., Volger, J. B., and Block, J. E. Radiology of osteoporosis. In: Riggs, B. L. and Melton. E. J., Eds. Osteoporosis. Etiology and Management. New York: Raven: 1993: 181-220.
14. Girasole. G., Jilka, R. E., Passeri. G., Boswell, S., Boder, G., Williams, D. C., and Manolagas, S. C. I7β-estradiol inhibits interleukin-6 production by bone marrow-derived stromal cells and osteoblasts in vitro: a potential mechanism for the antiosteoporotic effect of estrogens. J Clin Invest 89:883-891: 1992.
15. Kimble, R. B., Vannice, J. L., Bloedow, D. C., Thompson. R. C., Hopfer. W., Kung, V. T., Brownfield, C., and Pacifici, R. Interleukin-1 receptor antagonist decreases bone loss and bone resorption ill ovariectomised rats. J Clin Invest 93:1959-1967; 1994.
16. Kitazawa, R., Kimble, R., Vannice, J. L., Kung. V. T., and Pacifici. R. Interleukin I-receptor antagonist and tumor necrosis binding protein decrease osteoclast formation and bone resorption in ovariectomised rats. J Clin Invest 94:2397-2406: 1994.
17. Kurihara. N., Bertolini. D., Suda, T., Akayama. Y., and Roodman. G. D. IL-6 stimulated osteoclast-like multinucleated cell formation in long term human cultures by inducing IL-1 release. J Immunol 144:4226-4230; 1990.
18. Lai, L. C., Kadory. S., Siraj, A., and Lennard, T. W. J. Oncostastin M. interleukin-2, interleukin-6 and interleukin-8 in breast fluid. Int J Cancer 59:369-372; 1994.
19. Liel. Y., Edwards. J., Shary. J., Spicer. K. M., Gordon, B., and Bell, n. H. The effects of race and body habitus on bone mineral density of the radius, hip, and spine in premenopausal women. J Clin Endocrinol Metab 66:1247-1250; 1988.
20. Lindsay. R., Hart. D. M., Forrest, C., and Baird. C. Prevention of spinal osteoporosis in oophorectomized women. Lancet ii:1151-1153: 1980.
21. Marshall, D. H., Crilly, R. G., and nordin. B. E. C. Plasma androstenedione and oestrone levels in normal and osteoporotic postmenopausal women. Br Med J 2:1177-1179; 1977.
22. Martel. C., Rheaune. E., Takahashi, M., Trudcl. C., Couet. J., Luu-The, V., Simard, J., and Labrie, F. Distribution of 17 β HSD gene expression and activity in rat and human tissues. J Steroid Biochem Mol Biol 41:597-603; 1992.
23. Miller, W. R. and O'neill, J. S. The importance of local synthesis of estrugen within the breast. Steroids 50:537-547: 1987.
24. Nawata, H., Tanaka, S., Tanaka. S., Takayanagi, R., Sakai. Y., Yanase, T., Ikuyama. S., and Haji. M. Aromatase in bone cell: Association with osteoporosis in postmenopausal women. J Steroid Biochem Mol Biol 53:165-174; 1995.
25. Pelc. B., Marshall. D. H., Guha. P., Khan. M. Y., and nordin, B. E. C. The relation between plasma androstenedione. plasma oestrone and androstenedione to oestrone conversion rates on postmenopausal women with and without fractures. Clin Sci Mol Med 54:125-131: 1978.
26. Prefontaine. M., Shih, C., Pan, C. C., and Bhavnani, B. R. Applicability of the product isolation and radiometric aromatase assays for the measurement of low levels of aromatase: Lack of aromatase activity in the human endometrium. J Endocrinol 127:539-551; 1990.
27. Purohit. A., Flanagan, A. M., and Reed, M. J. Estrogen synthesis by osteoblast cell lines. Endocrinology 131:2027-2029: 1992.
28. Purohit. A., Ghilchik. M. W., Duncan. L., Wang. D. Y., Singh, A., Walker, M. M., and Reed, M. J. Aromatase activity and interleukin-6 production by normal and malignant breast tissue. J Clin Endocrinol Metab 80:3052-3058: 1995.
29. Ralston, S. H. Analysis of gene expression in human bone biopsies by polymerase chain reaction: Evidence for enhanced cytokine expression in postmenopausal osteoporosis. J Bone Miner Res 9:883-890; 1994.
30. Reed, M. J., Coldham, n. G., Patel. S., Ghilchik. M. W., and James. V. H. T. Interleukin-I and interleukin-6 in breast cyst fluid: Their role in regulating aromatase activity in breast cancer cells. J Endocrinol 132:R5-R8: 1993.
31. Ribot. C., Tremollieres. F., Pouilles. J. M., Bonneu. M., Germain. F., and Louvet, J. P. Obesity and postmenopausal bone loss: The influence of obesity on vertebral density and bone turnover in postmenopausal women. Bone 8:327-331; 1988.
32. Richelson, L. S., Heinze, H. W., Melton. L. J., III. and Riggs. B. L. Relative contributions of ageing and estrogen deficiency to postmenopausal bone loss. N Engl J Med 311:1273-1275; 1984.
33. Riggs, B. L., Ryan. R. J., Wahner, H. W., Jiang, n., and Mattox, V. R. Serum concentrations of estrogen. testosterone and gonadotrophins in osteoporotic and nonosteoporotic postmenopausal women. J Clin Endocrinol Metab 36:1097-2003; 1973.
34. Sambrook, J., Fritsch. E. F., and Maniatis. T. Molecular Cloning: A Eaboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press: 1989; Chap. 5.
35. Slemenda, C., Longcope, C., Peacock, M., Hui. S., and Johnston. C. C. Sex steroids, bone mass and bone loss. J Clin Invest 97:14-21; 1996.
36. Tamura, T., Udagawa. N., Takahashi, n., Miyauia, C., Tanaka. S., Yamada, Y., Koishihara. Y., Ohsugi, Y., Kumaki, K., Taga. T., Kishimoto, T., and Suda. T. Soluble interleukin-6 triggers osteoclast formation by interleukin 6. Proc natl Acad Sci USA 90:11924-11928; 1993.
37. Thomson B. M., Saklatvala. J., and Chambers. T. J. Osteoblasts mediate interleukin 1 stimulation of bone resorption by rat osteoclasts. J Exp Med 164:104-112: 1986.
38. Vittek, J., Altman. K., Gordon. G. G., and Southren. A. L. The metabolism of 17 alpha-3-H testosterone by rat mandibular bone. Endocrinology 94:325-329: 1974.
39. Whalen. G. F. Solid tumours and wounds: Transformed cells misunderstood as injured tissue. Lancet 336:1489-1492; 1990.
40. Zhou. D., Pompon, D., and Chen, S. Stable expression of human aromatase complementary DnA in mammalian cells: A useful system for aromatase inhibitor screening. Cancer Res 50:6949-6954: 1990.