PCR detection of Clostridium perfringens producing different toxins in faeces of goats

Letters in Applied Microbiology

Volumn 25, Issue 5, 1997, Pages 339-344

  Uzal, F A ^a   Plumb, J J ^a   Blackall, L L ^a   Kelly, W R ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM; METABOLITES; TOXIC MATERIALS;

AGAROSE GEL; AMPLICONS; CLOSTRIDIUM PERFRINGENS; DNA MARKERS; GENES ENCODING; MICRO-ORGANISMS; NEUTRALISATION; POLYMERASE CHAIN REACTION TECHNIQUES; PURE CULTURE;

POLYMERASE CHAIN REACTION;

TOXIN;

ANIMAL TISSUE; ARTICLE; BACTERIUM IDENTIFICATION; CLOSTRIDIUM PERFRINGENS; CONTROLLED STUDY; FECES ANALYSIS; GOAT; NONHUMAN; POLYMERASE CHAIN REACTION; TECHNIQUE;

ANIMALIA; BACTERIA (MICROORGANISMS); CAPRA HIRCUS; CLOSTRIDIUM PERFRINGENS;

EID: 0030710559     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1997.00247.x     Document Type: Article

References (15)

1. Daube, G., China, B., Simon, P., Hvala, K. and Mainil, J. (1994) Typing of Clostridium perfringens by in vitro amplification of toxin genes. Journal of Applied Bacteriology 77, 650-655.
2. Fach, P. and Guillou, J. P. (1993) Detection by in vitro amplification of the alpha-toxin (phospholipase C) gene from Clostridium perfringens. Journal of Applied Bacteriology 74, 61-66.
3. Havard, H.L., Hunter, S.E.C. and Titball, R.W. (1992) Comparison of the nucleotide sequence and development of a PCR test for the epsilon toxin gene of Clostridium perfringens type B and type D. FEMS Microbiology Letters 97, 77-82.
4. Hunter, S.E.C., Clarke, I.N., Kelly, D.C. and Titball, R.W. (1992) Cloning and nucleotide sequencing of the Clostridium perfringens epsilon-toxin gene and its expression in Escherichia coli. Infection and Immunity 60, 102-110.
5. Kozaki, S., Dufrenne, J., Hagenaars, A. M. and Notermans, S. (1979) Enzyme linked immunosorbent assay (ELISA) for the detection of Clostridium perfringens type B toxin. Japanese Journal of Medical Sciences and Biology 32, 199-205.
6. Møller, K. and Ahrens, P. (1996) Comparison of toxicity neutralisation, ELISA and PCR tests for typing of Clostridium perfringens and detection of the enterotoxin gene by PCR. Anaerobe 2, 103-110.
7. Niilo, L. (1980) Clostridium perfringens in animal disease: a review of current knowledge. Canadian Veterinary Journal 21, 141-148.
8. Perelle, S., Gibert, M., Boquet, P. and Popoff, M.R. (1993) Characterisation of Clostridium perfringens iota-toxin genes and expression in Escherichia coli. Injection and Immunity 61, 5147-5156.
9. Saiki, R.K., Bugawan, T.L., Horn, G.T., Mullis, K.B. and Erlich, H.A. (1986) Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes. Nature 324, 163-166.
10. Songer, J.G. (1996) Clostridial enteric diseases of domestic animals. Clinical Microbiology Reviews 9, 216-234.
11. Sterne, M. and Batty, I. (1975) Pathogenic Clostridia. London: Butherworth & Co.
12. Titball, R.W., Hunter, S.E., Martin, K.L. et al. (1989) Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens. Infection and Immunity 57, 367-376.
13. Uzal, F.A. and Kelly, W.R. (1996) Enterotoxaemia in goats. Veterinary Research Communications 29, 481-492.
14. Uzal, F.A., Plumb, J.J., Blackall, L.L., O'Boyle, D. and Kelly, W.R. (1996) Detection by polymerase chain reaction of Clostridium perfringens producing epsilon toxin in faeces and in gastrointestinal contents of goats. Letters in Applied Microbiology 23, 13-17.
15. Yoo, H.S., Lee, S.U., Park, K.Y. and Park, Y.H. (1997) Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR. Journal of Clinical Microbiology 35, 228-232.