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note
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2 (7.5%) incubator at 37°C. Microinjections were performed in culture medium buffered with 20 mM Na-HEPES, pH 7.4. For injections a Zeiss Microinjection Workstation (AIS) was used along with thin borosilicate glass capillaries with filaments (Hilgenberg) having a diameter of ≤ 0.5 μm at the tip. Distribution of the fluorophore was observed 30 min after injection with a confocal laser scanning microscope (CLSM) based on the MRC 500 system (BioRad) with an excitation wavelength at 488 nm.
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