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Volumn 36, Issue 20, 1997, Pages 2238-2241

Chemoenzymatic Synthesis of Fluorescent N-Ras Lipopeptides and Their Use in Membrane Localization Studies in Vivo

Author keywords

Fluorescence spectroscopy; Lipoproteins; Membranes; Ras proteins; Signal transduction

Indexed keywords


EID: 0030708812     PISSN: 05700833     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.199722381     Document Type: Article
Times cited : (33)

References (47)
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    • note
    • 2 (7.5%) incubator at 37°C. Microinjections were performed in culture medium buffered with 20 mM Na-HEPES, pH 7.4. For injections a Zeiss Microinjection Workstation (AIS) was used along with thin borosilicate glass capillaries with filaments (Hilgenberg) having a diameter of ≤ 0.5 μm at the tip. Distribution of the fluorophore was observed 30 min after injection with a confocal laser scanning microscope (CLSM) based on the MRC 500 system (BioRad) with an excitation wavelength at 488 nm.


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