Non-palindromic attl sites of integrons are capable of site-specific recombination with one another and with secondary targets

Molecular Microbiology

Volumn 26, Issue 3, 1997, Pages 441-453

  Hansson, Karin ^a   Sköld, Ola ^a   Sundström, Lars ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

RECOMBINASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; GENETIC RECOMBINATION; INTEGRON; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POINT MUTATION; PRIORITY JOURNAL; R PLASMID;

BACTERIA (MICROORGANISMS);

EID: 0030700665     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.5401964.x     Document Type: Article

References (43)

1. IMP. Antimicrob Agents Chemother 39: 1612-1615.
2. Argos, P., Landy, A., Abremski, K., Egan, J.B., Haggard-Ljungquist, E., Hoess, R.H., Kahn, M.L., Kalionis, B., Narayana, S.V.L., Pierson, S.L., Sternberg, N., and Leong, J.M. (1986) The integrase family of site-specific recombinases: regional similarities and global diversity. EMBO J 5: 433-440.
3. Bartolomé, B., Jubete, Y., Martinez, E., and de la Cruz, F. (1991) Construction and properties of a family of pACYC-derived cloning vectors compatible with pBR322 and its derivatives. Gene 102: 75-78.
4. Bennett, R.J., and West, S.C. (1995) RuvC protein resolves Holliday junctions via cleavage of the continuous (non-crossover) strands. Proc Natl Acad Sci USA 92: 5635-5639.
5. Bissonnette, L., and Roy, P.H. (1992) Characterization of In0 of Pseudomonas aeruginosa plasmid pVS1, an ancestor of integrons of multiresistance plasmids and transposons of Gram-negative bacteria. J Bacteriol 174: 1248-1257.
6. Cabezon, E., Lanka, E., and de la Cruz, F. (1994) Requirements for mobilization of plasmids RSF1010 and ColE1 by the IncW plasmid R388: trwB and RP4 traG are interchangeable. J Bacteriol 176: 4455-4458.
7. Cameron, F.H., Obbink, D.J.G., Ackerman, V.P., and Hall, R.M. (1986) Nucleotide sequence of the AAD (2′) amino-glycoside acetyltransferase determinant aadB. Evolutionary relationship of this region with those surrounding aadA in R538-1 and dhfrll in R388. Nucleic Acids Res 14: 8625-8635.
8. Collis, C.M., and Hall, R.M. (1992a) Site-specific deletion and rearrangement of integron insert genes catalyzed by the DNA integrase. J Bacteriol 174: 1574-1585.
9. Collis, C.M., and Hall, R.M. (1992b) Gene cassettes from the insert region of integrons are excised as covalently closed circles. Mol Microbiol 6: 2875-2885.
10. Collis, C.M., Grammaticopoulos, C., Briton, J., Stokes, H.W., and Hall, R.M. (1993) Site-specific insertion of gene cassettes into integrons. Mol Microbiol 9: 41-52.
11. Drew, H.R., and Travers, A.A. (1986) DNA bending and its relation to nucleosome positioning. J Mol Biol 186: 773-790.
12. Francia, M.V., and Lobo, J.M.G. (1996a) An inverted repeat in the Tn21 integron determines the production of stemloop DNA. Plasmid 35: 31-36.
13. Francia, M.V., and Lobo, J.M.G. (1996b) Gene integration in the Escherichia coli chromosome mediated by Tn27 integrase (Int21). J Bacteriol 178: 894-898.
14. Francia, M.V., de la Cruz, F., and Lobo, J.M.G. (1993) Secondary sites for integration mediated by the Tn21 integrase. Mol Microbiol 10: 823-828.
15. Grinsted, J., de la Cruz, F., and Schmitt, R. (1990) The Tn21 subgroup of bacterial transposible elements. Plasmid 24: 163-189.
16. Hall, R.M., and Collis, C.M. (1995) Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination. Mol Microbiol 15: 593-600.
17. Levesque, C., Brassard, S., Lapointe, J., and Roy, P.H. (1994) Diversity and relative strength of tandem promoters for the antibiotic resistance genes of several integrons. Gene 142: 49-54.
18. Martinez, E., and de la Cruz, F. (1988) Transposon Tn21 encodes a RecA-independent site-specific integration system. Mol Gen Genet 211: 320-325.
19. Martinez, E., and de la Cruz, F. (1990) Genetic elements involved in Tn27 site-specific integration, a novel mechanism for the dissemination of antibiotic resistance genes. EMBO J 9: 1275-1281.
20. Mizuuchi, K., Mizuuchi, M., and Gellert, M. (1982) Cruciform structures in palindromic DNA are favoured by DNA supercoiling. J Mol Biol 156: 229-243.
21. Müller-Hill, B., Crapo, L., and Gilbert, W. (1968) Mutants that make more lac represssor. Proc Natl Acad Sci USA 59: 1259-1264.
22. Nagaraja, R., and Weisberg, R.A. (1990) Specificity determinants in the attachment sites of bacteriophages HK022 and lambda. J Bacteriol 172: 6540-6550.
23. Nobuta, K., Tolmasky, M.E., Crosa, L.M., and Crosa, J.H. (1988) Sequencing and expression of the 6′-N-acetyltransferase gene of transposon Tn 1331 from Klebsiella pneumoniae. J Bacteriol 170: 3769-3773.
24. Ochman, H., Gerber, A.S., and Hartl, D.L. (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120: 621-623.
25. Ouellette, M., and Roy, P.H. (1987) Homology of ORFs from Tn2603 and from R46 to site-specific recombinases. Nucleic Acids Res 15: 10055.
26. Ouellette, M., Bissonnette, L., and Roy, P.H. (1987) Precise insertion of antibiotic resistance determinants into Tn27-like transposons: nucleotide sequencing of the OXA-1 β-lactamase gene. Proc Natl Acad Sci USA 84: 7378-7382.
27. Paulsen, I.T., Littlejohn, T.G., Rådström, P., Sundström, L., Sköld, O., Swedberg, G., and Skurray, R. (1993) The 3́ conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants. Antimicrob Agents Chemother 37: 761-768.
28. Rådström, P., Sköld, O., Swedberg, G., Flensburg, J., Roy, P.M., and Sundström, L. (1994) Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements. J Bacteriol 176: 3257-3268.
29. Recchia, G.D., and Hall, R.M. (1995a) Gene cassettes: a new class of mobile element. Microbiology 141: 3015-3027.
30. Recchia, G.D., and Hall, R.M. (1995b) Plasmid evolution by acquisition of mobile gene cassettes: plasmid pIE723 contains the aadB gene cassette precisely inserted at a secondary site in the IncQ plasmid RSF1010. Mol Microbiol 15: 179-187.
31. Recchia, G.D., Stokes, H.W., and Hall, R.M. (1994) Characterization of specific and secondary recombination sites recognised by the integron DNA integrase. Nucleic Acids Res 22: 2071-2078.
32. Rice, P.A. (1997) Making DNA do a U-turn: IHF and related proteins. Curr Opin Struct Biol 7: 86-93.
33. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
34. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
35. Scott, J.R., Bringel, F., Marra, D., van Alstine, G., and Rudy, C.K. (1994) Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol Microbiol 11: 1099-1108.
36. Sundström, L., and Sköld, O. (1990) The dhfrl trimethoprim resistance gene can be found at specific sites in other genetic surroundings. Antimicrob Agents Chemother 34: 642-650.
37. Sundström, L., Rådström, P., Swedberg, G., and Sköld, O. (1988) Site-specific recombination promotes linkage between trimethoprim and sulfonamide resistance genes. Sequence characterization of dhfrV and sull and a recombination active locus of Tn21. Mol Gen Genet 213: 191-201.
38. Sundström, L., Roy, P.M., and Sköld, O. (1991) Site-specific insertion of three structural gene cassettes in transposon Tn7. J Bacteriol 173: 3025-3028.
39. Swift, G., MacCarthy, B.J., and Heffron, F. (1981) DNA sequence of a plasmid-encoded dihydrofolate reductase. Mol Gen Genet 181: 441-447.
40. Vandeyar, M.A., Weiner, M.P., Mutton, C.J., and Batt, C.A. (1988) A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants. Gene 65: 129-133.
41. Wong, W.M., Au, D.M.Y., Lam, V.M.S., Tam, J.W.O., and Cheng, L.Y.L. (1990) A simplified and improved method for the efficient double-stranded sequencing of mini-prep plasmid DNA. Nucleic Acids Res 18: 5573.
42. Wylie, B.A., and Koornhof, H.J. (1991) Nucleotide sequence of dihydrofolate reductase type VI. J Med Microbiol 35: 214-218.
43. Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.