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Volumn 278, Issue 5337, 1997, Pages 467-471

Mediation of classical conditioning in Aplysia californica by long-term potentiation of sensorimotor synapses

Author keywords

[No Author keywords available]

Indexed keywords

N METHYL DEXTRO ASPARTIC ACID RECEPTOR;

EID: 0030700543     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5337.467     Document Type: Article
Times cited : (128)

References (62)
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    • DL-APV (Sigma, St. Louis, MO) was dissolved in normal ASW to a final concentration of 100 μM and perfused into the recording chamber immediately after Pretest 3. It was washed out immediately after Test 7.
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    • Summary statistics are presented as mean ± SEM; n denotes number of preparations. Statistical significance for multiple group comparisons was assessed by either parametric or nonparametric [Kruskal-Wallis (KW)] analysis of variance (ANOVA). The choice of statistical test was determined by the outcome of Bartlett's tests for homogeneity of variances performed on the group data. Subsequent between-group comparisons were made with Student-Newman-Keuls tests (parametric) or Dunn's tests (nonparametric). Planned comparisons between two experimental groups were made with either unpaired t tests or nonparametric Mann-Whitney tests. Intragroup comparisons (between pre-and posttests) were made with Wilcoxon signed-rank tests. All reported levels of statistical significance represent two-tailed values.
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    • The mean amplitude of the Test alone EPSP was 44.2 ± 8% for the 15-min posttest and 43.7 ± 11% for the 60-min posttest. Both of the posttest EPSPs were smaller than the Test alone EPSP on Pretest 3 (P < 0.01 for each comparison). The mean amplitudes of the Test alone-APV EPSP were 61.0 ± 10% for the 15-min posttest and 65.6 ± 7% for the 60-min posttest. Both of these posttest EPSPs were also smaller than the Test alone-APV EPSP on Pretest 3 (P < 0.01 for each comparison).
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    • +-APV EPSP = 8.4 ± 1.6 mV; Test alone EPSP = 5.4 ± 0.7 mV; Test alone-APV EPSP = 7.1 ± 1.4 mV; F(3,29) = 1.3594, P > 0.2].
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    • Because there were no significant differences between the Test alone and the Test alone-APV groups in the paired-training experiments (Fig. 1, B and C) (17, 18), we did not include a Test alone-APV group in the unpaired-training experiments.
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    • The mean EPSP for the Test alone group was 51.0 ± 9% on the 15-min posttest and 75.3 ± 18% on the 60-min posttest. The Test alone EPSP was smaller on the 15-min posttest than on Pretest 3 (P < 0.04). The difference between the EPSPs for Pretest 3 and the 60-min posttest was not significant (P > 0.1).
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    • --APV EPSP = 4.0 ± 0.9 mV; Test alone EPSP = 4.4 ± 1.2 mV; F(2,28) = 0.2094, P > 0.81).
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    • Data not shown
    • Data not shown.
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    • We thank M. Barish, M. Fanselow, F. Krasne, and T. O'Dell for their helpful comments on the manuscript. Supported by grants from the National Institutes of Health (NS29563), the National Science Foundation (IBN-9410579), the Alzheimer's Disease Program, State of California Department of Health Services (95-23335), and the Academic Senate, University of California, Los Angeles, to D.L.G. as well as by a grant from the National Institute of Mental Health (F31-MH11136) to G.G.M.


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