Mitochondrial function in oncogene-transfected rat fibroblasts isolated from multicellular spheroids

American Journal of Physiology - Cell Physiology

Volumn 273, Issue 5 42-5, 1997, Pages

  Kunz Schughart, L A ^a,b   Habbersett, R C ^a   Freyer, J P ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Cell cycle; Flow cytometry; Mitochondria; Rat embryo fibroblasts

Indexed keywords

ANIMAL CELL; ARTICLE; CELL ISOLATION; CELL METABOLISM; CONTROLLED STUDY; ENERGY METABOLISM; FIBROBLAST CULTURE; FLOW CYTOMETRY; GENETIC TRANSFECTION; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NONHUMAN; ONCOGENE; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0030698779     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1997.273.5.c1487     Document Type: Article

References (19)

1. Acker, H., and J. Carlsson. The microenvironment in multicellular spheroids. In: Spheroid Culture in Cancer Research, edited by R. Bjerkvig. Boca Raton, FL: CRC, 1992, p. 41-56.
2. Bourrat-Floeck, B., K. Groebe, and W. Mueller-Klieser. Biological response of multicellular EMT6 spheroids to exogenous lactate. Int. J. Cancer 47: 792-799, 1991.
3. Bredel-Geissler, A., U. Karbach, S. Walenta, L. Vollrath, and W. Mueller-Klieser. Proliferation-associated oxygen consumption and morphology of tumor cells in monolayer and spheroid culture. J. Cell. Physiol. 153: 44-52, 1992.
4. Crissman, H. A., and J. A. Steinkamp. Anew method for rapid and sensitive detection of bromodeoxyuridine in DNA-replicating cells. Exp. Cell Res. 173: 256-261, 1987.
5. Evenson, D. P., J. Lee, Z. Darzynkiewicz, and M. R. Melamed. Rhodamine 123 alters the mitochondrial ultrastructure of cultured L1210 cells. J. Histochem. Cytochem. 33: 353-359, 1985.
6. Freyer, J. P. Spheroids in radiobiology research. In: Spheroid Culture in Cancer Research, edited by R. Bjerkvig. Boca Raton, FL: CRC, 1992, p. 217-275.
7. Freyer, J. P. Rates of oxygen consumption for proliferating and quiescent cells isolated from multicellular spheroids. Adv. Exp. Med. Biol. 345: 335-342, 1994.
8. Freyer, J. P., and P. Schor. Regrowth kinetics of cells from different regions of multicellular spheroids of four cell lines. J. Cell. Physiol. 138: 384-392, 1989.
9. Freyer, J. P., and P. Schor. Automated selective dissociation of cells from different regions of multicellular spheroids. In Vitro Cell. Dev. Biol. Anim. 25: 9-19, 1989.
10. Freyer, J. P., P. L. Schor, K. A. Jarrett, M. Neeman, and L. O. Sillerud. Cellular energetics measured by phosphorous NMR spectroscopy are not correlated with chronic nutrient deficiency in multicellular tumor spheroids. Cancer Res. 51: 3831-3837, 1991.
11. Freyer, J. P., and R. M. Sutherland. Selective dissociation and characterization of cells from different regions of multicell tumor spheroids. Cancer Res. 40: 3956-3965, 1980.
12. Freyer, J. P., and R. M. Sutherland. A reduction in the in situ rates of oxygen and glucose consumption of cells in EMT6/Ro spheroids during growth. J. Cell. Physiol. 124: 516-524, 1985.
13. Freyer, J. P., and R. M. Sutherland. Regulation of growth saturation and development of necrosis in EMT6/Ro multicell spheroids by the glucose and oxygen supply. Cancer Res. 46: 3504-3512, 1986.
14. Groebe, K., and W. Mueller-Klieser. Distributions of oxygen, nutrient, and metabolic waste concentrations in multicellular spheroids and their dependence on spheroid parameters. Eur. Biophys. J. 19: 169-181, 1991.
15. 31P nuclear magnetic resonance and toxicity studies. Cancer Res. 50: 6936-6943, 1990.
16. Johnson, L. V., M. L. Walsh, B. J. Bokus, and L. B. Chen. Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy. J. Cell Biol. 83: 526-535, 1981.
17. Johnson, L. V., M. L. Walsh, and L. B. Chen. Localization of mitochondria in living cells with rhodamine 123. Proc. Natl. Acad. Sci. USA 77: 990-994, 1980.
18. Kallinowski, F., G. Tyler, W. Mueller-Klieser, and P. Vaupel. Growth-related changes of oxygen consumption rates of tumor cells grown in vitro and in vivo. J. Cell. Physiol. 138: 183-191, 1989.
19. Kessel, D., W. T. Beck, D. Kukuruga, and V. Schulz. Characterization of multidrug resistance by fluorescent dyes. Cancer Res. 51: 4665-4670, 1991.