Hyaluronan synthase of chlorella virus PBCV-1

Science

Volumn 278, Issue 5344, 1997, Pages 1800-1803

  DeAngelis, Paul L ^a   Jing, Wei ^a   Graves, Michael V ^b   Burbank, Dwight E ^b   Van Etten, James L ^b  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATE; GENE PRODUCT; HYALURONIC ACID DERIVATIVE; POLYMER; SYNTHETASE;

AMINO ACID SEQUENCE; CHLORELLA VIRUS; ENZYME ACTIVITY; GENE EXPRESSION; GREEN ALGA; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; REVIEW; VIRUS GENOME; VIRUS INFECTION;

AMINO ACID SEQUENCE; CHLORELLA; GENES, VIRAL; GLUCURONOSYLTRANSFERASE; GLUTAMINE-FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING); GLYCOSYLTRANSFERASES; HYALURONIC ACID; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PHYCODNAVIRIDAE; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; TRANSFERASES; URIDINE DIPHOSPHATE GLUCOSE DEHYDROGENASE; XENOPUS PROTEINS;

ALGAE; ANIMALIA; CHLORELLA VIRUS; CHLOROPHYTA; ESCHERICHIA COLI; PARAMECIUM BURSARIA CHLORELLA VIRUS 1;

EID: 0030692815     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5344.1800     Document Type: Review

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37. 10 cells) were infected with PBCV-1 (multiplicity of infection of 5) and incubated for 50 or 90 min after infection. Another culture served as an uninfected control. The cells were harvested, and the membrane fraction (yield ∼3 mg of protein) was prepared as described [P. L. DeAngelis and A. M. Achyuthan, J. Biol. Chem. 271, 23657 (1996)], except that 1 mM mercaptoethanol was substituted for dithiothreitol. The paper chromatography method was used to assay for HAS activity (13).
38. We thank A. M. Achyuthan, G. M. Air, M. K. Brakke, R. D. Cummings, L. C. Lane, M. Nelson, and P. H. Weigel for helpful discussions. R. A. Steinberg provided the plasmids and host strain for T7 expression system. Supported by a NIH grant (R01-GM56497) and a University of Oklahoma Medical Alumni Scholarship to P.L.D. and a NIH grant (R01-GM32441) to J.V.E.