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Volumn 278, Issue 5338, 1997, Pages 672-675

Requirement for CD44 in activated T cell extravasation into an inflammatory site

Author keywords

[No Author keywords available]

Indexed keywords

HERMES ANTIGEN; HYALURONIC ACID; MONOCLONAL ANTIBODY; STAPHYLOCOCCUS ENTEROTOXIN B;

EID: 0030690228     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5338.672     Document Type: Article
Times cited : (497)

References (35)
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    • 0028085152 scopus 로고
    • J. Sprent, Cell 76, 315 (1994).
    • (1994) Cell , vol.76 , pp. 315
    • Sprent, J.1
  • 19
    • 1842403425 scopus 로고    scopus 로고
    • H. C. DeGrendele, P. Estess, M. H. Siegelman, data not shown
    • H. C. DeGrendele, P. Estess, M. H. Siegelman, data not shown.
  • 20
    • 1842371364 scopus 로고    scopus 로고
    • note
    • 7 labeled cells per mouse in 0.5 ml of HBSS with or without the addition of blocking reagent. Depletions were done as above. Cells treated with whole antibody or Fab fragments were incubated in a saturating concentration of antibody for 15 min on ice and then washed before injection. HA and chondroitin sulfate (Sigma) were added to the cell suspension at the time of injection (final concentration, 0.5 μM). Hyaluronidase (ICN Biochemicals) and chondroitinase ABC (Sigma) treatment was done by injecting 10 U per mouse iv 30 min before donor cell infusion. Ninety minutes after the infusion of labeled cells, the recipient mice were killed and PELs were analyzed for the homing of labeled cells.
  • 31
    • 1842289063 scopus 로고    scopus 로고
    • note
    • 3, and 0.1 mM EDTA. PELs were collected by peritoneal lavage using 5 ml of RPMI (37°C) containing 2% FBS and 2 mM EDTA. Staining of PELs was performed as above, except cells were preincubated with anti-CD32/CD16 (clone 2.4G2) and stained in the presence of 10% normal mouse serum to inhibit Fc receptor interactions. Data were collected on a FACScan analytical instrument (Becton Dickinson) and analyzed using Lysis II software.
  • 35
    • 1842330189 scopus 로고    scopus 로고
    • note
    • We thank M. Kosfiszer for technical assistance and L. Picker for helpful discussions. Supported by grants from NIH (R01 CA57471 and HL56746), the Arthritis Foundation, and the Welch Foundation (I-1227). M.H.S. is an Established Investigator of the American Heart Association. H.C.D. is supported by an NIH Cancer Immunology Training Grant.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.