β1, 4 N-Acetylgalactosaminyltransferase (GM2/GD2/GA2 synthase) forms homodimers in the endoplasmic reticulum: A strategy to test for dimerization of Golgi membrane proteins

Glycobiology

Volumn 7, Issue 7, 1997, Pages 987-996

  Zhu, Guofen ^a   Jaskiewicz, Ewa ^a   Bassi, Rosaria ^b   Darling, Douglas S ^a   Young Jr , William W ^c  

^a UNIVERSITY OF LOUISVILLE   (United States)

^b INSTITUTE OF IMMUNOLOGY AND EXPERIMENTAL THERAPY   (Poland)

^c UNIVERSITY OF MILAN   (Italy)

Author keywords

Ganglioside; Glycosyltransferase

Indexed keywords

N ACETYLGALACTOSAMINYLTRANSFERASE;

ANIMAL CELL; ARTICLE; CELL FRACTIONATION; CHO CELL; CONTROLLED STUDY; DIMERIZATION; DISULFIDE BOND; ENDOPLASMIC RETICULUM; ENZYME STRUCTURE; GOLGI COMPLEX; IMMUNOBLOTTING; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE;

ANIMALS; BLOTTING, WESTERN; CELL EXTRACTS; CHO CELLS; CRICETINAE; DIMERIZATION; ENDOPLASMIC RETICULUM; FLUORESCENT ANTIBODY TECHNIQUE; GOLGI APPARATUS; MEMBRANE PROTEINS; N-ACETYLGALACTOSAMINYLTRANSFERASES; PRECIPITIN TESTS; TRANSFECTION;

ANIMALIA;

EID: 0030681358     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/7.7.987     Document Type: Article

References (51)

1. Arar. C., Carpentier. V., Le Caer, J. -P., Monsigny. M., Legrand. A. and Roche. A. C. (1995) ERGIC-53, a membrane protein of the endoplasmic reticulumGolgi intermediate compartment, is identical to MR60, an intracellular mannose-specific lectin of myelomonocytic cells. J. Biol. Client., 270, 3551-3553.
2. Bannykh, S., Aridor. M., PIutner. H., Rowe. T. and Balch. W. E. (1995) Regulated export of cargo from the endoplasmic reticulum of mammalian cells. Cold Spring Harbor Symp. Quant. Biol., 60, 127-137.
3. Berninsone. P., Lin, Z. -Y., Kempner, E. and Hirschberg. C. B. (1995) Regulation of yeast Golgi glycosylation. Guanosine diphosphatase functions as a homodimer in the membrane. J. Biol. Cliein., 270, 14564-14567.
4. Bretscher. M. S. and Munro. S. (1993) Cholesterol and the Golgi apparatus. Science, 261, 1280-1281.
5. ChamberlainJ. P. (1979) Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal. Biochem., 98, 132-135.
6. Evan. G. I., Lewis. G. K., Ramsay, G. and Bishop, J. M. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell Dial, 5, 3610-3616.
7. Fleischer. B., McIntyre, J. O. and Kempner. E. S. (1993) Target sizes of galacto-syltransferase, sialyltransferase, and uridine diphosphatase in Golgi apparatus of rat liver. Biochemistry, 32, 2076-2081.
8. Habuchi, H., Habuchi. O. and Kimata, K. (1995) Purification and characterization of heparan sulfate 6-sulfotransferase from the culture medium of Chinese hamster ovary cells. J. Biol. Chem., 270, 4172-4179.
9. Hammond, C. and Helenius. A. (1995) Quality control in the secretory pathway. Ciirr. Opin. Cell Biol., 7, 523-529.
10. Haraguchi. M., Yamashiro. S., Yamamoto. A., Furukawa. K., Takamiya. K., Lloyd, K. O. and Shiku. H. (1994) Isolation of GD3 synthase gene by expression cloning of GM3 alpha-2, 8-sialyItransferase cDNA using anti-GD2 monoclonal antibody. Proc. Natl. Acad. Sci. USA, 91, 10455-10459.
11. Hashimoto. Y., Sekine. M., Iwasaki. K. and Suzuki, A. (1993) Purification and characterization of UDP-N-acetylgalactosamine GM3/GD3 Nacetylgalactosaminyltransferase from mouse liver. J. Biol. Client., 268, 25857-25864.
12. Haslam. D. B. and Baenziger. J. U. (1996) Expression cloning of Forssman glycolipid synthetase: A novel member of the histo-blood group ABO gene family. Proc. Natl. Acad. Sci. USA, 93, 10697-10702.
13. Henion. T. R., Macher, B. A., Anaraki. F. and Galili. U. (1994) Defining the minimal size of catalytically active primate a 1, 3 galactosyltransferase: structurefunction studies on the recombinant truncated enzyme. Glycobiology, 4, 193-202.
14. Ichikawa, S., Sakiyama. H., Suzuki. G., Hidari. K. I. P. J. and Hirabayashi. Y. (1996) Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis. Proc. Natl. Acad Sci. USA, 93, 4638-4643.
15. Jackson, M. R., Nilsson, T. and Peterson. P. A. (1993) Retrieval of transmembrane proteins to the endoplasmic reticulum. J. Cell Biol., 121, 317-333.
16. Jaskiewicz. E., Zhu. G., Bassi. R., Darling, D. S. and Young. W. W. (1996a) βl, 4 N-acetylgalactosaminyltransferase (GM2 synthase) is released from Golgi membranes as a neuraminidase sensitive, disulfide-bonded dimer by a cathepsin D-like protease. J. Biol. Client., 271, 26395-26403.
17. Jaskiewicz. E., Zhu, G., Taatjes. D. J., Darling, D. S., Zwanzig, G. E., and Young. W. W. (1996b) Cloned βl, 4 N-acetylgalactosaminyltransferase: subcellular localization and formation of disulfide bonded species. Glycoconjugate J., 13, 213-223.
18. Kempner. E. S. (1993) Novel predictions from radiation target analysis. Trends Kochern. Sei., 18, 236-239.
19. Lipari. F. and Herscovics. A. (1996) Role of the cysteine residues in the alphal, 2-mannosidase involved in N-glycan biosynthesis in Saccharomyces cerevisiae. J. Biol. Client., 271, 27615-27622.
20. Lodish. H. F. (1988) Transport of secretory and membrane glycoproteins from the rough endoplasmic reticulum to the Golgi. J. Biol. Cltem., 263, 2107-2110.
21. Loo, T. W. and Clarke, D. M. (1996) The minimum functional unit of human P-glycoprotein appears to be a monomer. J. Biol. Client., 271, 27488-27492.
22. Lotteau. V., Teyton. L., Peleraux. A., Nilsson. T., Karlsson, L., Schmid. S., Quaranta, V. and Peterson. P. A. (1990) Intracellular transport of class II MHC molecules directed by invariant chain. Nature, 348, 600-605.
23. Ma, J. Y. and Colley. K. J. (1996) A disulfide-bonded dimer of the Golgi βgalactoside a2, 6-sialyltransferase is catalytically inactive yet still retains the ability to bind galactose. J. Biol. Client., 271, 7758-7766.
24. Machamer, C. E. (1993) Targeting and retention of Golgi membrane proteins. Curr. Opin. Cell Biol., 5, 606-612.
25. Malissard, M., Borsig. L., DiMarco. S., Griitter. M. G., Kragl. U., Wandrey. C. and Berger. E. G. (1996) Recombinant soluble beta-l, 4-galactosyltransferases expressed in Saccharomyces cerevisiae: purification, characterization and comparison with human enzyme. Ear. J. Biochem., 239, 340-348.
26. Mandon, E. C, Kempner. E. S., Ishihara. M. and Hirschberg. C. B. (1994a) A monomeric protein in the Golgi membrane catalyzes both W-deacetylation and W-sulfation of heparan sulfate. J. Biol. Client., 269, 11729-11733.
27. Mandon, E. C, Milla. M. E., Kempner. E. and Hirschberg. C. B. (1994b) Purification of the Golgi adenosine 3'-phosphate 5'-phosphosulfate transporter, a homodimer within the membrane. Proc. Natl. Acad. Sci. USA, 91, 1070710711.
28. Moremen. K. W., Touster. O. and Robbins. P. W. (1991) Novel purification of the catalytic domain of Golgi alpha-mannosidase II: characterization and comparison with the intact enzyme. J. Biol. Client. . 266, 16876-16885.
29. Munro. S. (1995) A comparison of the transmembrane domains of Golgi and plasma membrane proteins. Biochem. Soc. Trans., 23, 527-530.
30. Munro, S. and Pelham. H. R. B. (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell, 48, 899-907.
31. Nagata, Y., Yamashiro. S., Yodoi, J., Lloyd. K. O. and Furukawa. K. (1992) Expression cloning of beta 1, 4 N-acetylgalactosaminyltransferase cDNAs that determine the expression of GM2 and GD2 gangliosides. J. Biol. Client., 267, 12082-12089.
32. Nagata. Y., Yamashiro, S., Yodoi. J., Lloyd, K. O., Shiku, H. and Furukawa. K. (1994) Correction: expression cloning of β1, 4 N-acetylgalactosaminyltrans-ferase cDNAs that determine the expression of GM2 and GD2 gangliosides. J. Biol. Chem., 269, 7045
33. Nara. K., Watanabe. Y., Maruyama. K., Kasahara, K., Nagai. Y. and Sanai, Y. (1994) Expression cloning of a CMP-NeuAc:NeuAca2-3Galβl-4Glcβ1-1'Cer a2, 8-sialyltransferase (GD3 synthase) from human melanoma cells. Proc. Natl. Acad. Sci. USA, 91, 7952-7956.
34. Navaratnam, N., Ward. S., Fisher. C., Kuhn, N. J., Keen. J. N. and FindlayJ. B. C. (1988) Purification, properties and cation activation of galactosyltransferase from lactating-rat mammary Golgi membranes. Enr. J. Biochem., 171, 623-629.
35. Nilsson, T., Pypaert, M., Hoe. M. H., Slusarewicz. P., Berger. E. G. and Warren, G. (1993a) Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells. J. Cell Biol, 120, 5-13.
36. Nilsson, T., Slusarewicz. P., Hoe. M. H. and Warren. G. (1993b) Kin recognition: a model for the retention of Golgi enzymes. FEBS Lett., 330, 1-4.
37. Nilsson. T., Hoe. M. H., Slusarewicz. P., Rabouille. C., Watson. R., Hunte, P., Watzele. G., Berger. E. G. and Warren. G. (1994) Kin recognition between medial Golgi enzymes in HeLa cells. EMBO J., 13, 562-574.
38. Rose, J. K. and Doms, R. W. (1988) Regulation of protein export from the endoplasmic reticulum. Anna. Rev. Cell Biol., -4, 257-288.
39. Roth. J. (1987) Subcellular organization of glycosylation in mammalian cells. Biochim. Biophys. Ada, 906, 405-436.
40. Roth, J., Taatjes, D. J., Weinstein, J., PauIson. J. C., Greenwell. P. and Watkins. W. M. (1986) Differential subcompartmentation of terminal glycosylation in the Golgi apparatus of intestinal absorptive and goblet cells. J. Biol. Chem., 261, 14307-14312.
41. Sasaki. K., Kurata, K., Kojima. N., Kurosawa. N., Ohta. S., Hanai. N., Tsuji. S. and Nishi. T. (1994) Expression cloning of a GM3-specific alpha-2, 81 sialyltransferase (GD3 synthase). J. Biol Chem., 269, 15950-15956.
42. Schulte. S. and Stoffel. W. (1993) Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression. Proc. Natl. Acad. Sci. USA, 90, 10265-10269.
43. Schutze, M. -P., Peterson. P. A. and Jackson. M. R. (1994) An N-terminal double-arginine motif maintains type II membrane proteins in the endoplasmic reticulum. EMBO J., 13, 1696-1705.
44. Smith. P. K., Krohn. R. I., Hermanson. G. T., Mallic. A. K., Gartner, F. H., Provenzano. M. D., Fujimoto. E. K., Goeke. N. M., Olson. B. J. and Klenk. D. C. (1985) Anal. Biochem., 150, 76-85.
45. Stahl, N., Jurevics. H., Morell. P., Suzuki. K. and Popko, B. (1994) Isolation, characterization, and expression of cDNA clones that encode rat UDP-galactose: ceramide galactosyltransferase. J. Neurosci. Res., 38, 234-242.
46. Sullivan, K. F., Hechenberger. M. and Masri, K. (1994) Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere. J. Cell Biol., 127, 581-592.
47. Teasdale. R. D. and Jackson. M. R. (1996) Signal-mediated sorting of membrane proteins between the endoplasmic reticulum and the Golgi apparatus. Annn. Rev. Cell Biol., 12, 27-54.
48. Townsley. F. M. and Pelham. H. R. B. (1994) The KKXX signal mediates retrieval of membrane proteins from the Golgi to the ER in yeast. Eur. J. Cell Biol., 211-216.
49. Wilson. I. A., Niman. H., Houghten. R., Cherenson. A., ConnolIy. M. and Lerner, R. (1984) The structure of an antigenic determinant in a protein. Cell, 37, 767-778.
50. Yamaguchi. N. and Fukuda, M. N. (1995) Golgi retention mechanism of β-1, 4 galactosyltransferase. J. Biol. Chem., 270, 12170-12176.
51. Young, W. W., (1993) Molecular approaches to studying the intracellular trafficking of glycosphingolipids. Adv. Lipid Res., 26, 161-179.