Mutants of Ralstonia (Pseudomonas) solanacearum sensitive to antimicrobial peptides are altered in their lipopolysaccharide structure and are avirulent in tobacco

Journal of Bacteriology

Volumn 179, Issue 21, 1997, Pages 6699-6704

  Titarenko, Elena ^a   López Solanilla, Emilia ^a   García Olmedo, Francisco ^a   Rodríguez Palenzuela, Pablo ^a  

^a Electrotechnical Section   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL VIRULENCE; ENZYME ACTIVITY; GENE MUTATION; LIPID TRANSPORT; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PSEUDOMONAS; TOBACCO;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NICOTIANA TABACUM; PSEUDOMONAS; RALSTONIA SOLANACEARUM; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0030680976     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.21.6699-6704.1997     Document Type: Article

References (62)

1. Baker, C. J., M. J. Neilson, L. Sequeira, and K. G. Keegstra. 1984. Chemical characterization of the lipopolysaccharide of Pseudomonas solanacearum. Appl. Environ. Microbiol. 47:1096-1100.
2. Blatny, J. Unpublished data.
3. Bohlmann, H., S. Clausen, S. Behnke, H. Giese, C. Hiller, G. U. Reimann-Philipp, Schrader, V. Barkholt, and K. Apel. 1988. Leaf-specific thionins of barley: a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants. EMBO J. 7:1559-1565.
4. Bowles, D. J. 1990. Defense-related proteins in higher plants. Annu. Rev. Biochem. 59:873-909.
5. Buddenhagen, I., and A. Kelman. 1964. Biological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 2:203-230.
6. Carmona, M. J., A. Molina, J. A. Fernández, J. J. López-Fando, and F. García-Olmedo. 1993. Expression of the α-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. Plant J. 3:457-462.
7. Carrasco, L., D. Vázquez, C. Hernández-Lucas, P. Carbonero, and F. García-Olmedo. 1981. Thionins: plant peptides that modify membrane permeability in cultured mammalian cells. Eur. J. Biochem. 116:185-189.
8. Clore, G. M., D. K. Sukumaran, A. M. Cronenborn, M. M. Teeter, M. Whitlow, and B. L. Jones. 1987. Nuclear magnetic resonance study of the solution structure of α1purothionin. J. Mol. Biol. 193:571-578.
9. Cook, D., and L. Sequeira. 1991. Genetic and biochemical characterization of a Pseudomonas solanacearum gene cluster required for extracellular polysaccharide production and for virulence. J. Bacteriol. 173:1654-1662.
10. Daniels, M. J., C. E. Barber, P. C. Turner, M. K. Sawczyz, R. J. W. Byrde, and A. H. Fielding. 1984. Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1. EMBO J. 3:3323-3328.
11. Díaz, I., M. J. Carmona, and F. García-Olmedo. 1992. Effects of thionins on β-glucuronidase in vitro and in plant protoplasts. FEBS Lett. 296:279-282.
12. Dow, M. J., A. E. Osbourn, T. J. G. Wilson, and M. J. Daniels. 1995. A locus determining pathogenicity of Xanthomonas campestris is involved in lipopolysaccharide biosynthesis. Mol. Plant-Microbe Interact. 8:768-777.
13. Fernández de Caleya, R., B. González-Pascual, F. García-Olmedo, and P. Carbonero, P. 1972. Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro. Appl. Microbiol. 23:998-1000.
14. García-Olmedo, F., P. Rodríguez-Palenzuela, C. Hernández-Lucas, F. Ponz, C. Maraña, M. J. Carmona, J. López-Fando, J. A. Fernández, and P. Carbonero. 1989. The thionins: a protein family that includes purothionin, viscotoxins and crambins. Oxford Surv. Plant Mol. Cell Biol. 6:31-60.
15. García-Olmedo, F., M. J. Carmona, J. J. López-Fando, J. A. Fernández, A. Castagnaro, A. Molina, C. Hernández-Lucas, and P. Carbonero. 1992. Characterization and analysis of thionin genes, p. 283-302. In E. Boller and F. Meins (ed.), Genes involved in plant defense. Springer-Verlag, Vienna.
16. García-Olmedo F., A. Molina, A. Segura, and M. Moreno. 1995. The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol. 3:72-74.
17. García-Olmedo, F., A. Molina, A. Segura, M. Moreno, A. Castagnaro, E. Titarenko, P. Rodríguez-Palenzuela, M. Piñeriro, and I. Díaz. 1996. Engineering plants against pathogens: a general strategy. Field Crops Res. 45: 79-84.
18. Granada, G. A., and L. Sequeira. 1983. A new selective medium for Pseudomonas solanacearum. Plant Dis. 67:1084-1088.
19. Goldman, R. C., and L. Leive. 1980. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur. J. Biochem. 107:145-153.
20. Hejgaard, S., S. Jacobsen, and I. Svendsen. 1991. Two antifungal thaumatin-like proteins from barley grain. FEBS Lett. 291:127-131.
21. Higgins, D. G., and P. M. Sharp. 1989. Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151-153.
22. Kao, C. C., and L. Sequeira. 1991. A gene cluster required for coordinated biosynthesis of lipopolisaccharide and extracellular polysaccharide also affects virulence of Pseudomonas solanacearum. J. Bacteriol. 173:7841-7847.
23. Kelman, A. 1954. The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44:693-695.
24. Kingsley, M. T., D. W. Gabriel, G. C. Marlow, and P. D. Roberts. 1993. The opsX locus of Xanthomonas campestris affects host range and biosynthesis of lipopolysaccaride and extracellular polysaccharide. J. Bacteriol. 175:5839-5845.
25. Leah, R., H. Tommerup, I. Svendsen, and J. Mundy. 1991. Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J. Biol. Chem. 266:1464-1473.
26. Macías, E., F. Rana, J. Blazyk, and M. C. Modrzakowski. 1990. Bactericidal activity of magainin 2: use of lipopolysaccharide mutants. Can. J. Microbiol. 36:582-584.
27. Mauch, F., L. A. Hadwiger, and T. Boller. 1988. Antifungal hydrolases in pea tissue. Plant Physiol. 87:325-333.
28. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
29. Molina, A., and F. García-Olmedo. 1991. Novel antipathogenic peptides and compositions. Patent application P9101258 (24.05.91) PCT/EP92/01130. U.S. patent 5,446,127 (8.29.1995).
30. Molina, A., and F. García-Olmedo. 1993. Developmental and pathogen-induced expression of three barley genes encoding lipid transfer proteins. Plant J. 4:983-991.
31. Molina, A., P. Ahl-Goy, A. Fraile, R. Sánchez-Monge, and F. García-Olmedo. 1993. Inhibition of bacterial and fungal pathogens by thionin of types I and II. Plant Sci. 92:169-177.
32. Molina, A., A. Segura, and F. García-Olmedo. 1993. Lipid transfer proteins from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. FEES Lett. 316:119-122.
33. Molina, A., and F. García-Olmedo. Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2. Plant J., in press.
34. Moreno, M., A. Segura, and F. García-Olmedo. 1994. Pseudothionins-St1, a potato peptide active against potato pathogens. Eur. J. Biochem. 233:135-139.
35. Moreno, M. 1995. Thesis dissertation. Universidad Politécnica de Madrid, Madrid, Spain.
36. Mueller, P. R., and B. Wold. 1989. In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science 246:780-786.
37. Newman, M. A., J. Conrads-Strauch, G. Scofield, M. J. Daniels, and J. M. Dow. 1994. Defense-related gene induction in Brassica campestris in response to defined mutants of Xanthomonas campestris with altered pathogenicity. Mol. Plant-Microbe Interact. 7:553-563.
38. Nikaido, H. 1989. Outer membrane barrier as a mechanism of antimicrobial resistance. Antimicrob. Agents Chemother. 33:1831-1836.
39. Osborn, M. J. 1963. Studies on the gram-negative cell wall, I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolisaccharide of Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 50:449-506.
40. Osorio, E., V. R. Castro, and L. P. Vernon. 1989. Hemolytic activity of thionin from Pyrularia pubera nuts and snake venom toxins of Naja species: Pyrularia thionin and snake venom cardioxin compete for the same membrane site. Toxicon 27:511-517.
41. Palva, T., and C. R. Makela. 1980. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate/polyacrylamide electrophoresis. Eur. Biochem. 107:137-144.
42. Pearson, W. R., and D. J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444-2448.
43. Piñeiro, M., F. García-Olmedo, and I. Díaz. 1994. Redox modulation of the expression of bacterial genes encoding cysteine-rich proteins in plant protoplasts. Proc. Natl. Acad. Sci. USA 91:3867-3871.
44. Piñeiro, M., I. Díaz, P. Rodríguez-Palenzuela, E. Titarenko, and F. García-Olmedo. 1995. Selective disulphide linkage of plant thionins with other proteins. FEBS Lett. 369:239-242.
45. Ponz, F., C. Hernández-Lucas, P. Carbonero, and F. García-Olmedo. 1982. Lipid-binding proteins from the endosperms of wheat and oats. Phytochemistry 23:2178-2181.
46. Rick, P. 1987. Lipopolysaccharide biosynthesis, p. 648-662. In F. C. Neidhart, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. ASM Press, Washington, D.C.
47. Rothmel, R. K., A. M. Chakrabarty, A. Berry, and A. Darzins. 1991. Genetics systems in Pseudomonas. Methods Enzymol. 204:485-514.
48. Roncero, C., and M. J. Casadaban. 1992. Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus. J. Bacteriol. 174:3250-3260.
49. Rudd, K. E. 1993. Maps, genes, sequences and computers: an Escherichia coli case study. ASM News 59:335-341.
50. Sambrook, J., E. F. Fritsch, and T. Maniatis, T. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
51. Schlumbaum, A., F. Mauch, U. Vögeli, and T. Boller. 1986. Plant chitinases are potent inhibitors of fungal growth. Nature 234:365-367.
52. Segura, A., M. Moreno, and F. García-Olmedo. 1993. Purification and antipathogenic activity of lipid transfer proteins (LTPs) from leaves of Arabidopsis and spinach. FEBS Lett. 332:243-246.
53. Sela-Buurlage, M. B., A. S. Ponstein, S. A. Bres-Vloemans, L. S. Melchers, P. J. M. van den Elzen, and B. J. C. Corneliessen. 1993. Only specific tobacco (Nicoliana tabacum) chitinases and β-1,3-glucanases exhibit antifungal activity. Plant Physiol. 101:857-863.
54. Simon, R., U. Priefer, and A. Puhler. 1983. A broad host range mobilization system for in vivo genetic engineering transposon mutagenesis in gram-negative bacteria. BioTechnology 1:784-791.
55. Sirisena, D. M., P. R. MacLachlan, S. H. Liu, A. Hessel, and K. E. Sanderson. 1994. Molecular analysis of the rfaD gene, for heptose synthesis, and the rfaF gene, for heptose transfer, in lipopolysaccharide synthesis in Salmonella typhymurium. J. Bacteriol. 176:2379-2385.
56. Terras, F. R. G., I. J. Goderis, F. Van Leuven, J. Vanderleyden, B. P. A. Cammue, and W. F. Broekaert. 1992. In vitro antifungal activity of a radish (Raphanus salivus L.) seed protein homologous to nonspecific lipid transfer proteins. Plant Physiol. 100:1055-1058.
57. Terras, F. R. G., H. M. E. Schoofs, M. F. C. De Bolle, F. Van Leuven, S. B. Rees, J. Vanderleyden, B. P. A. Cammue, and W. F. Broekaert. 1992. Analysis of two novel classes of antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 267:15301-15309.
58. Tsai, C. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
59. Vigers, A. J., W. K. Roberts, and C. P. Selitrennikoff. 1991. A new family of plant antifungal proteins. Mol. Plant-Microbe Interact. 4:315-323.
60. Whitlow, M., and M. M. Teeter. 1985. Energy minimization for tertiary structure prediction of homologous proteins: α 1-purothionin and viscotoxin A3 and crambin. J. Biomol. Struct. Dynam. 2:831-848.
61. Woloshuk, C. P., J. S. Meulenhoff, M. Sela-Buurlage, P. J. M. van den Elzen, and B. J. C. Cornelissen. 1991. Pathogen-induced proteins with inhibitory activity toward Phytophthora infestans. Plant Cell 3:619-628.
62. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-111.