The detection of esterolytically active glycerophospholipid: Cholesterol acyltransferase of Aeromonas salmonicida in SDS-polyacrylamide gels using the α-naphthyl acetate-azo dye method

Journal of Fish Diseases

Volumn 20, Issue 6, 1997, Pages 459-461

  Lachmann, I ^a   Wagner, U ^a   Hadge O ^a   Drossler K ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AEROMONAS SALMONICIDA;

EID: 0030680920     PISSN: 01407775     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2761.1997.00318.x     Document Type: Article

References (9)

1. Buckley J.T. (1982) Substrate specificity of bacterial glycerophospholipid: cholesterol acyltransferase. Biochemistry 21. 6699-6703.
2. Buckley J.T. (1983) Mechanism of action of bacterial glycerophospholipid: cholesterol acyltransferase. Biochemistry 22, 5490-5493.
3. Buckley J.T., Halasa L.N. & MacIntyre S. (1982) Purification and partial characterization of a bacterial phospholipid: cholesterol acyltransferase. Journal of Biological Chemistry 257, 3320-3325.
4. Eggset G., Bjørnsdottir R., McQueen L.R., Arnesen J.A., Coucheron D.H. & Jørgensen T.Ø. (1994) Extracellular grycerophospholipid: cholesterol acyltransferase from Aeromonas salmonicida. activation by serine protease. Journal of fish Diseases 17, 17-29.
5. Laemmli U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
6. Lee K.K. & Ellis A.A. (1989) The quantitative relationship of lethality between extracellular protease and haemolysin of Aeromonas salmonicida in Atlantic salmon, Salmo salar. FEMS Microbiological Letters 61, 127-132.
7. Lee K.K. & Ellis A.E. (1990) Glycerophospholipid: cholesterol acyltransferase complexed with lipopolysaccharide (LPS) is a major lethal exotoxin and cytolysin of Aeromonas salmonicida: LPS stabilizes and enhances toxicity of the enzyme. Journal of Bacteriology 172, 5382-5393.
8. Nachlas M.M. & Seligman A.M. (1949) The histochemical demonstration of esterase. Journal of the National Cancer Institute 9, 415-425.
9. Towbin H., Staehelin T. & Gordon J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proceedings of the National Academy of Sciences of the USA 76, 4350-4354.