Single-cell gel electrophoresis (SCG)-A review and discussion

Environmental Health and Preventive Medicine

Volumn 2, Issue 2, 1997, Pages 53-58

  Lu, Yuquan ^a   Takeshita, Tatsuya ^a   Morimoto, Kanehisa ^a  

^a OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

Author keywords

Biomarker; Comet assay; DNA strand breaks; Genotoxicity; Single cell gel electrophoresis

Indexed keywords

BIOLOGICAL MARKER;

CANCER THERAPY; CARCINOGENESIS; CHROMOSOME ABERRATION; DIAGNOSTIC TEST; DNA POLYMORPHISM; DNA REPAIR; DNA STRAND BREAKAGE; ENVIRONMENTAL FACTOR; GEL ELECTROPHORESIS; GENOTOXICITY; MONITORING; REVIEW; SISTER CHROMATID EXCHANGE;

EID: 0030678146     PISSN: 1342078X     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931964     Document Type: Review

References (75)

1. Singh NP, McCOY MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184-91.
2. Duthie SJ, Ma A, Ross MA, Collins AR. Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Res 1996; 56: 1291-5.
3. Pandrangi R, Petras M, Ralph S, Vrzoc M. Alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp. Environ Mol Mutagen 1995; 26: 234-56.
4. Plappert U, Raddatz K, Roth S, Fliedner TM. DNA-damage detection in man after radiation exposure - the comet assay - its possible application for human biomonitoring. Stem Cells (Dayt) 1995; 13 Suppl 1: 215-22.
5. Tice RR, Strauss GH. The single cell gel electrophoresis/comet assay: A potential tool for detecting rediarion-induced DNA damage in humans. Stem Cells (Dayt) 1995; 13 Suppl 1: 207-14.
6. Olive PL, Vikse CM, Durand RE. Hypoxic fractions measured in murine tumors and normal tissues using the comet assay. Int J Radiat Oncol Biol Phys 1994; 29: 487-91.
7. Olive PL, Durand RE, Le Riche J, Olivotto IA, Jackson SM. Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers. Cancer Res 1993; 53: 733-6.
8. Tice RR, Strauss GH, Peters WP. High-dose combination alkylating agents with autologous bone-marrow support in patients with breast cancer preliminary assessment of DNA damage in individual peripheral blood lymphocytes using the single cell gel electrophoresis assay. Mutat Res 1992; 271: 101-13.
9. Delaney CA, Green MH, Lowe JE, Green IC. Endogenous nitric oxide induced by interleukin-1 beta in rat islets of Langerhans and HIT-T15 cells causes significant DNA damage as measured by the 'comet' assay. FEBS Lett 1993; 333: 291-5.
10. Gedik CM, Ewen SW, Collins AR. Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int J Radiat Biol 1992; 62: 313-20.
11. Sardas S, Walker D, Akyol D, Karakaya AE. Assessment of smoking-induced DNA damage in lymphocytes of smoking mothers of newborn infants using the alkaline single-cell gel electrophoresis technique. Mutat Res 1995; 335: 213-7.
12. Betti C, Davini T, Giannessi L, Loprieno N, Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 mornal subjects. Mutat Res 1994; 307: 323-33.
13. Betti C, Davini T, Giannessi L, Loprieno N, Barale R. Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat Res 1995; 343: 201-7.
14. Hartmann A, Plappert U, Raddatz K, Grunert Fuchs M, Speit G. Does physical activity induce DNA damage? Mutagenesis 1994; 9: 269-72.
15. Meyers CD, Fairbairn DW, O'Neill KL. Measuring the repair of H2O2-induced DNA single strand breaks using trie single cell gel assay. Cytoblos 1993; 74: 147-53.
16. Fairbairn DW, Reyes WA, Van Grigsby R, O'Neill KL. Laser scanning microscopic analysis of DNA damage in frozen tissues. Cancer Lett 1994; 76: 127-32.
17. Collins AR, Ma AG, Duthie SJ. The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutat Res 1995; 336: 69-77.
18. Holz O, Jorres R, Kastner A, Krause T, Magnussen H. Reproducibility of basal and induced DNA single-strand breaks detected by the single-cell gel electrophoresis assay in human peripheral mononuclear leukocytes. Int Arch Occup Environ Health 1995; 67: 305-10.
19. Holz O, Jorres R, Kastner A, Magnussen H. Differences in basal and induced DNA single-strand breaks between human peripheral monocytes and lymphocytes. Mutat Res 1995; 332: 55-62.
20. Barber CV, Fantel AG. The role of oxygenation in embryotoxic mechanisms of three bioreducible agents. Teratology 1993; 47: 209-23.
21. Pfuhler S, Wolf HU. Detection of DNA-crosslinking agents with the alkaline comet assay. Environ Mol Mucagen 1996; 27: 196-201.
22. Anderson D, Yu TW, Schmezer P. An investigation of the DNA-damaging ability of benzene and its metabolites in human lymphocytes, using the comet assay. Environ Mol Mucagen 1995; 26: 305-14.
23. Muller WU, Bauch T, Streffer C, Niedereichholz F, Bocker W. Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines. Int J Radiat Biol 1994; 65: 315-9.
24. Plewa MJ, Wagner ED, Yu TW, Anderson D. Genotoxicity of m-phenylenediamine and 2-aminofluorene in Salmonella typhimurium and human lymphocytes with and without plant activation. Environ Mol Mutagen 1995; 26: 171-7.
25. Taj S, Nagarajan B. Induction of genotoxicity by salted deep-fried fish and mutton. Mutat Res 1994; 322: 45-54.
26. Green MH, Lowe JE, Waugh AP, Aldridge KE, Cole J, Arlett CF. Effect of diet and vitamin C on DNA strand breakage in freshly-isolated human white blood cells. Mutat Res 1994; 316: 91-102.
27. Hartmann A, Niess AM, Grunert Fuchs M, Poch B, Speit G. Vitamin E prevents exercise-induced DNA damage. Mutat Res 1995; 346: 195-202.
28. Anderson D, Yu TW, Phillips BJ, Schmezer P. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutat Res 1994; 307:261-71.
29. Plappert U, Barthel E, Seidal HJ. Reduction of benzene toxicity by toluene. Environ Mol Mutagen 1994; 24: 283-92.
30. Verschaeve L, Gilles J. Single cell gel electrophoresis assay in the earthworm for the earthworm for the detection of genotoxic compounds in soils. Bull Environ Contam Toxicol 1995; 54: 112-9.
31. Koppen G L. Verschaeve. The alkaline comet test on plant cells: A new genotoxicity test for DNA strand breaks in Vicia faba root cells. Mutat Res 1996; 360: 193-200.
32. Fairbairn D, Meyers D, O'Neill K. Detection of DNA damaging agents in environmental water samples. Bull Environ Contam Toxicol 1994; 52: 687-90.
33. Pool Zobel BL, Guigas C, Klein R, Neudecker C, Renner HW, Schmezer P. Assessment of genotoxic effects by lindane. Food Chem Toxicol 1993; 31: 271-83.
34. Ribas G, Frenzilli G, Barale R, Marcos R. Herbicide-induced DNA damage in human lymphocytes evaluated by the single-cell gel electrophoresis (SCGE) assay. Mutat Res 1995; 344: 41-54.
35. Hejmadi MV, McKeown SR, Friery OP, McIntyre IA, Patterson LH, Hirst DG. DNA damage following combination of radiation with the bioreductive drug AQ4N: Possible selective toxicity to oxic and hypoxic tumour cells. Br J Cancer 1996; 73: 499-505.
36. de With A, Leitz G, Greulich KO. UV-Bk-laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresis. J Photochem Photobiol B 1994; 24: 47-53.
37. Vijayalaxmi, Strauss GH, Tice RR. An analysis of gamma-ray-induced DNA damage in human blood leukocytes, lymphocytes and granulocytes. Mutat Res 1993; 292: 123-8.
38. Vijayalaxmi, Tice RR, Strauss GH. Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gel electrophoresis technique. Mutat Res 1992; 271: 243-52.
39. Jostes RF, Hui TE, Cross FT. Single-cell gel technique supports hit probability calculations. Health Phys 1993; 64: 675-9.
40. de With A, Greulich KO. Wavelength dependence of laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresis. J Photochem Photobiol B 1995; 30: 71-6.
41. Hara A, Zhang W, Kobayashi H, Niikawa S, Sakai N, Yamada H. A single cell gel electrophoresis technique for the detection of DNA damage induced by ACNU, an alkylating agent or irradiation in murine glioma cell lines. Neurol Res 1994; 16: 234-40.
42. Olive PL, Banath JP. Radiation-induced DNA double-strand breaks produced in histone-depleted tumor cell nuclei measured using the neutral comet assay. Radiat Res 1995; 142: 144-52.
43. Arlett CF, Lowe JE, Harcourt SA, Waugh AP, Cole J, Roza L, Diffey BL, Mori T, Nikaido O, Green MH. Hypersensitivity of human lymphocytes to UV-B and solar irradiation. Cancer Res 1993; 53: 609-14.
44. Miller DL, Thomas RM, Buschbom RL. Comet assay reveals DNA strand breaks induced by ultrasonic cavitation in vitro. Ultrasound Med Biol 1995; 21: 841-8.
45. Kligerman AD, Mottus K, Erexson GL. Cytogenetic analyses of the in vitro and in vivo responses of murine cells to peroxyacetyl nitrate (PAN). Mutat Res 1995; 341: 199-206.
46. Ashby J, Tinwell H, Lefevre PA, Browne MA. The single cell gel electrophoresis assay for induced DNA damage (comet assay): Measurement of tail length and moment. Mutagenesis 1995; 10: 85-90.
47. Plappert U, Barthel E, Raddatz K, Seidel HJ. Early effects of benzene exposure in mice. Hematological versus versus genotoxic effects. Arch Toxicol 1994; 68: 284-90.
48. Vaghef H, Hellman B. Demonstration of chlorobenzene-induced DNA damage in mouse lymphocytes using the single cell gel electrophoresis assay. Toxicology 1995; 96: 19-28.
49. 6-guanine DNA adducts, DNA strand breaks and mutant frequencies in the hypoxanthine quanine phosphoribosyltransferase gene in T-lymphocytes. Carcinogenesis 1995; 16: 1473-81.
50. Bastlova T, Vodicka P, Peterkova K, Hemminki K, Lambert B. Styrene oxide-induced HPRT mutations, DNA adducts and DNA strand breaks in cultured human lymphocytes. Carcinogenesis 1995; 16: 2357-62.
51. De Meo M, Laget M, Castegnaro M, Dumenil G. Genotoxic activity of potassium permanganate in acidic solutions. Mutat Res 1991; 260: 295-306.
52. Singh NP, Tice RR, Stephens RE, Schneider EL. A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutat Res 1991; 252: 289-96.
53. Rojas E, Valverde M, Herrera LA, Altamirano Lozano M, Ostrosky Wegman P. Genotoxicity of vanadium pentoxide evaluate by the single cell gel electrophoresis assay in human lymphocytes. Mutat Res 1996; 359: 77-84.
54. Bjorge C, Wiger R, Holme JA, Brunborg G, Andersen R, Dybing E, Soderlund EJ. In vitro toxicity of 1, 2-dibromo-3-chloropropane (DBCP) in different testicular cell types from rats. Reprod Toxicol 1995; 9: 461-73.
55. Churg A, Keeling B, Gilks B, Porter S, Olive P. Rat mesothelial and tracheal epithelial cells show equal DNA sensitivity to hydrogen peroxide-induced oxidant injury. AmJ Physiol 1995; 268 (5 Pt 1): 1832-8.
56. Ward AJ, Olive PL, Burr AH, Rosin MP. Response of fibroblast cultures from ataxia-telangiectasia patients to reactive oxygen species generated during inflammatory reactions. Environ Mol Mutagen 1994; 24: 103-11.
57. Nocentini S. Comet assay analysis of repair of DNA strand breaks in normal and deficient human cells exposed to radiations and chemicals. Evidence for a repair pathway specificity of DNA ligation. Radiat Res 1995; 144: 170-80.
58. Olive PL, Durand RE. Detection of hypoxic cells in a murine tumor with the use of the comet assay. J Natl Cancer Inst 1992; 84: 707-11.
59. Hu Q, Kavanagh MC, Newcombe D, Hill RP. Detection of hypoxic fractions in murine tumors by comet assay: Comparison with other techniques. Radiat Res 1995; 144: 266-75.
60. Olive PL. Use of the comet assay to detect hypoxic cells in murine tumours and normal tissues exposed to bioreductive drugs. Acta Oncol 1995; 34: 301-5.
61. Peitsch MC, Muller C, Tschopp J. DNA fragmentation during apoptosis is caused by frequent single-strand cuts. Nucleic Acids Res 1993; 21: 4206-929.
62. Fairbairn DW, Carnahan KG, Thwaits RN, Grigsby RV, Holyoak GR, O'Neill KL. Detection of apoptosis induced DNA cleavage in scrapie-infected sheep brain. FEMS Microbiol Lett 1994; 115: 341-6.
63. Olive PL, Frazer G, Banath JP. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat Res 1993; 136: 130-6.
64. Gopalakrishna P, Khar A. Comet assay to measure DNA damage in apoptotic cells. J Biochem Biophys Methods 1995; 30: 69-73.
65. Musk SR, Asdey SB, Edwards SM, Stephenson P, Hubert RB, Johnson IT. Cytotoxic and clastogenic effects of benzyl isothiocyanate towards cultured mammalian cells. Food Chem Toxicol 1995; 33: 31-7.
66. Hartmann A, Speit G. Comparative investigations of the genotoxic effects of metals in the single cells gel (SCG) assay and the sister chromarid exchange (SCE) test. Environ Mol Mutagen 1994; 23: 299-305.
67. Kim JP, D'Arpa P, Jacobson Kram D, Williams JR. Ultraviolet-light exposure induces a heritable sensitivity to the induction of SCE by mitomycin-C. Mutat Res 1985; 149: 437-42.
68. Kligerman AD, Allen JW, Erexson GL, Morgan DL. Cytogenetic studies of rodents exposed to styrene by inhalation. IARC Sci Publ 1993; (127): 217-24.
69. Ward AJ, Olive PL, Burr AH, Rosin MP. A sensitivity to oxidative stress is linked to chromosome 11 but is not due to a difference in single strand DNA breakage or repair. Mutat Res 1993; 294: 299-308.
70. Malcolmson AM, Davies G, Hanson JA, Deeley JO, Gaffney CC, McGregor AD, Kerby IJ. Determination of radiation-induced damage in lymphocytes using the micronucleus and microgel electrophoresis 'Comet' assays. Eur J Cancer 1995; 31A: 2320-3.
71. Tuo J, Loft S, Thomsen MS, Poulsen HE. Benzene-induced genotoxicity in mice in vivo detected by the alkaline comet assay: Reduction by CYP2E1 inhibition. Mutat Res 1996; 368: 213-9.
72. Fairbairn D, O'Neill KL, Standing MD. Application of confocal laser scanning microscopy to analysis of H2O2-induced DNA damage in human cells. Scanning 1993; 15: 136-9.
73. O'Neill KL, Fairbairn DW, Standing MD. Analysis of single-cell gel electrophoresis using laser-scanning microscopy. Mutat Res 1993; 319: 129-34.
74. Kreja L, Selig C, Nothdurft W. Assessment of DNA damage in canine peripheral blood and bone marrow after total body irradiation using the single-cell gel electrophoresis technique. Mutat Res 1996; 359: 63-70.
75. Kamesaki S, Kamesaki H, Jorgensen TJ, Tanizawa A, Pommier Y, Cossman J. bcl-2 protein inhibits etoposide-induced apoptosis through its effects on events subsequent to topoisomerase II-induced DNA strand breaks and their repair [published erratum appears in Cancer Res 1994; 54: 3074]. Cancer Res 1993; 53: 4251-6.