Electron microscopy of biological specimens by the plasma-polymerization rapid-freeze replica method

Journal of Electron Microscopy

Volumn 46, Issue 5, 1997, Pages 425-430

  Yamaguchi, Masashi ^a   Hirokawa, Hideo ^b   Sugahara, Keishin ^c   Mizokami, Hiroshi ^c   Takeo, Kanji ^a  

^a CHIBA UNIVERSITY   (Japan)

^b SOPHIA UNIVERSITY   (Japan)

^c KAKETSUKEN   (Japan)

Author keywords

Hepatitis B virus core particle; Mica flakes method; Plasma polymerization; Rapid freeze; Replica; T4 bacteriophage

Indexed keywords

HEPATITIS B VIRUS; MICA; T4 BACTERIOPHAGE; UNIDENTIFIED BACTERIOPHAGE;

MICA; PLASMA POLYMERIZATION;

BIOLOGICAL SPECIMENS; CORE PARTICLES; HEPATITIS B VIRUS; HEPATITIS B VIRUS CORE PARTICLE; MICA FLAKE METHOD; MICA FLAKES; RAPID FREEZE; REPLICA; REPLICA METHOD; T4 BACTERIOPHAGES;

HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY;

EID: 0030670889     PISSN: 00220744     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jmicro.a023538     Document Type: Article

References (11)

1. Tanaka A (1991) Replica method with plasma polymerized hydrocarbon film by glow discharge. Denshikenbikyo (Electron Microsc.) 26: 141-150 (in Japanese).
2. Tanaka A, Yamaguchi M, and Hirano T (1989) New replica method with plasma polymerized film by glow discharge. In: Proceedings of 46th Annual Meeting of the Electron Microscopy Society of America, Milwaukee, ed. Bailer GW, pp. 414-415, (San Francisco Press, San Francisco).
3. Tanaka A, Yamaguchi M, Iwasaki T, and Iriyama K (1989) A new replica method for electron microscopic observation of Langmuire-Blodgett film with plasma polymerized film by glow discharge. Chem. Lett. 1989: 1219-1222.
4. Bresch C (1963) Laboratory Outline for a Course on Bacterial Virus, (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).
5. Imamura T, Sugahara K, Adachi S, Miyatsu Y, Mizokami H, and Matsusaka T (1988) Purification and characterization of the hepatitis B virus core antigen produced in the yeast Saccharomyces cerevisiae. J. Biotechnol. 8: 149-162.
6. Yamaguchi M, Tanaka A, and Suzuki T (1992) A support film of plasma-polymerized naphthalene for electron microscopy: method of preparation and application. J. Electron Microsc. 41: 7-13.
7. Yamaguchi M, Hirano T, Hirokawa H, Sugahara K, Mizokami H, and Matsubara K (1988) Cryo-electron microscopy of hepatitis B virus core particles produced by transformed yeast: comparison with negative staining and ultrathin sectioning. J. Electron Microsc. 37: 337-341.
8. Yamaguchi M, Hirano T, Sugahara K, Mizokami H, Araki M, and Matsubara K (1988) Electron microscopy of hepatitis B virus core antigen expressing yeast cells by freeze-substitution fixation. Eur. J. Cell Biol. 47: 138-143.
9. Heuser J E (1983) Procedure for freeze-drying molecules absorbed to mica flakes. J. Mol. Biol. 169: 155-195.
10. Yamaguchi M and Kondo I (1989) Immunoelectron microscopy of Proteus vulgaris by the plasma polymerization metal-extraction replica method: differential staining of flagellar (H) and somatic (O) antigens by colloidal golds. J. Electron Microsc. 38: 382-388.
11. Tanaka A (1994) Osmium conductive metal coating for SEM specimen using sublimated osmium tetroxide in negative glow phase of DC glow discharge. J. Electron Microsc. 43: 177-182.