A flow cytometric assay to monitor apoptosis in cell culture using a pH-sensitive fluoroprobe carboxy SNARF-1-AM

Genetic Engineer and Biotechnologist

Volumn 17, Issue 2-3, 1997, Pages 85-88

  Ishaque, A ^a   Slngh, R P ^a   Al Rubeai, M ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Apoptosis; Carboxy SNARF 1 AM; Fluoroprobe; Monitoring

Indexed keywords

CAMPTOTHECIN; FLUORESCENT DYE; GLUTAMINE; OXYGEN; STAUROSPORINE;

APOPTOSIS; CELL CULTURE; CELL PH; CELL POPULATION; CONFERENCE PAPER; CONTROLLED STUDY; ETIOLOGY; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; HYBRIDOMA; HYPOXIA; PH; PRIORITY JOURNAL;

ANIMALIA;

EID: 0030669093     PISSN: 0959020X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (9)

1. Al-Rubeai, M., Emery, A.N. & Chalder, S (1991) Flow cytometric study of cultured mammalian cells. Journal of Biotechnology, 19, 67-82.
2. Al-Rubeai, M., Singh, R.P., Goldman, M.H. et al. (1995) Death mechanisms of animal cells in conditions of agitation. Biotechnology & Bioengineering, 45, 463-472.
3. Arenas, M.J., Morris, R.G. & Wyllie, A.H. (1990) Apoptosis, the role of the endonuclease. American Journal of Pathology, 136, 593-608.
4. Barry, M.A., Behnke, C.A. & Eastman, A. (1990) Activation of programmed cell death (apoptosis) by cisplatin, other anticancer drugs, toxins and hyperthermia. Biochemical Pharmacology, 40, 2353-2362.
5. Kerr, J.F.R., Wyllie, A.H. & Currie, A.R. (1972) Apoptosis: a basic biological phenomenon with wide ranging implications in tissue kinetics. British Journal of Cancer, 26, 239-257.
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7. Singh, R.P., Al-Rubeai, M. & Emery, A.N. (1996) Apoptosis: exploiting novel pathways to the improvement of cell culture processes. The Genetic Engineer and Biotechnologist, 16, 227-251.
8. Welsh, J.P. & Al-Rubeai, M. (1996) The relationship between intracellular pH and cell cycle in cultured animal cells using SNARF-1 indicator. In Al-Rubeai, M. & Emery, A.N. (eds) Flow Cytometry Applications in Culture. Marcel Deckker, Inc., pp. 163-175.
9. Wyllie, A.H. (1980) Gluocorticoid-induced thymocyte apoptosis is associated with endogenous nuclease activation. Nature, 284, 555-556