Preparation of a 'beheaded' derivative of the 30S ribosomal subunit

Biochimie

Volumn 79, Issue 8, 1997, Pages 523-526

  Ulitin, A B ^a   Agalarov, S Ch ^a   Serdyuk, I N ^a  

^a INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

Author keywords

16S RNA domains; Derivative of the 30S subunit; RNase H cleavage

Indexed keywords

OLIGODEOXYRIBONUCLEOTIDE; PROTEIN SUBUNIT; RIBONUCLEASE H; RIBOSOME PROTEIN;

ARTICLE; CONTROLLED STUDY; ELECTROPHORESIS; NONHUMAN; PROTEIN DEFICIENCY; PROTEIN DEGRADATION; PROTEIN PURIFICATION; RIBOSOME SUBUNIT; THERMUS THERMOPHILUS; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0030667107     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(97)82745-9     Document Type: Article

References (18)

1. Noller H (1984) Structure of ribosomal RNA. Annu Rev Biochem 53, 119-162
2. Brimacombe R, Stiege W (1985) Structure and function of ribosomal RNA. Biochem J 229, 1-17
3. Noller H, Woese C (1981) Secondary structure of 16S ribosomal RNA. Science 212, 403-411
4. Brimacombe R, Atmadij J, Stiege W, Schuler D (1988) A detailed model of the three-dimensional structure of E coli 16S ribosomal RNA in situ in the 30S subunit. J Mol Biol 199, 115-136
5. Fink D, Chen R, Noller H, Altman R (1996) Computational methods for defining the allowed conformational space of 16S rRNA based on chemical footprinting data. RNA 2, 851-866
6. Spitnik-Elson P, Elson D (1979) The fragmentation of ribosomes. Methods Enzymol LIX, 461-481
7. Roth H, Nierhaus K (1973) Isolation of four ribonucleoprotein fragments from the 30S subunit of E coli ribosomes. FEBS Lett 31, 35-38
8. Samaha R, O'Brien B, O'Brien T, Noller H (1994) Independent in vitro assembly of a ribonucleoprotein particle containing the 3' domain of 16S RNA. Proc Natl Acad Sci USA 91, 7884-7888
9. Weitzmann C, Cunningham P, Nurse K, Ofengand J (1993) Chemical evidence for domain assembly of the Escherichia coli 30S ribosome. FASEB 7, 177-180
10. Afonina E, Chichkova N, Bogdanova S, Bogdanov A (1991) 30S ribosomal subunits with fragmented 16S RNA: a new approach for structure and function study of ribosomes. Biochimie 73, 777-787
11. Bogdanov A, Chichkova N, Kopilov A, Mankin A, Skripkin E (1988) Surface topography of ribosomal RNA. Methods Enzymol 164, 440-455
12. Oshima T, Imahori K (1974) Description of Thermus thermophilus (Yoshida and Oshima) comb nov, a non-sporulating thermophilic bactrium from Japanese thermal spa. Int J Syst Bacteriol 24, 102-112
13. Gogia Z, Yusupov M, Spirina T (1986) Structure of T thermophilus ribosomes. I. Method for isolation and purification. Molekulyarnaya Biologiya (in Russian) 20, 519-526
14. Eliseikina I, Agalarov S, Muranova T, Markova L, Kashparov I, Avliyakulov N, Garber M (1995) Preparative isolation of proteins from Thermus thermophilus ribosomal 30S subunits in non-denaturing conditions. Biokhimia (in Russian) 60, 1720-1730
15. Murzina N, Vorozheykina D, Matvienko N (1989) Nucleotide sequence of Thermus thermophilus HB8 gene coding 16S rRNA. Nucleic Acids Res 16, 8172
16. Geyl D, Bock A, Isono K (1981) An improved method for two-dimensional gel-electrophoresis: analysis of mutationally altered ribosomal proteins of E coli. Mol Gen Genet 181, 309-312
17. Tsiboli P, Herfurth E, Choli T (1994) Purification and characterization of the 30S ribosomal proteins from the bactrium Thermus thermophilus. Eur J Biochem 226, 169-177
18. Spirin A, Serdyuk I, Shpungin J, Vasiliev V (1979) Quaternary structure of the ribosomal 30S subunit: Model and its experimental testing. Proc Natl Acad Sci USA 76, 4867-4871