Site-directed mutagenesis of the Cys residues in ClpA, the ATPase component of protease Ti (ClpAP) in Escherichia coli

Biological Chemistry

Volumn 378, Issue 10, 1997, Pages 1205-1209

  Seol, Jae Hong ^a   Kwon, Jeong Ah ^a   Yoo, Soon Ji ^a   Kim, Hye Sun ^b   Kang, Man Sik ^a   Chung, Chin Ha ^a  

^a Seoul National University   (South Korea)

^b Ajou University   (South Korea)

Author keywords

ATP dependent protease; ATPase; ClpA; ClpP; Escherichia coli; N ethylmaleimide; Ti

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ALANINE; CASEIN; CYSTEINE; N ETHYLMALEIMIDE; PROTEINASE; SERINE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS;

ADENOSINE TRIPHOSPHATASES; ALANINE; CASEINS; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASE CLP; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ETHYLMALEIMIDE; MUTAGENESIS, SITE-DIRECTED; SERINE; SERINE ENDOPEPTIDASES; SULFHYDRYL REAGENTS;

ESCHERICHIA COLI;

EID: 0030665975     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Ames, B. (1966). Assays of inorganic phosphate, total phosphate and phosphatase. Methods Enzymol. 8, 115-118.
2. Chung, C.H., Seol, J.H., and Kang, M.S. (1996). Protease Ti (Clp), a multi-component ATP-dependent protease in Escherichia coli. Biol. Chem. 377, 549-554.
3. Goldberg, A.L. (1992). The mechanism and functions of ATP-dependent proteases in bacterial and animal cells. Eur. J. Biochem. 203, 9-23.
4. Gottesman, S., and Maurizi, M.R. (1992). Regulation by proteolysis: Energy-dependent proteases and their targets. Microbiol. Rev. 56, 592-621.
5. Gottesman, S., Clark, W.P., and Maurizi, M.R. (1990). The ATP-dependent Clp protease of Escherichia coli: Sequence of clpA and identification of Clp-specific substrates. J. Biol. Chem. 265, 7886-7893.
6. Hwang, B.J., Park, W.J., Chung, C.H., and Goldberg, A.L. (1987). Escherichia coli contains a soluble ATP-dependent protease (Ti) distinct from protease La. Proc. Natl. Acad. Sci. USA 84, 5550-5554.
7. Hwang, B.J., Woo, K.M., Goldberg, A.L., and Chung, C.H. (1988). Protease Ti, a new ATP-dependent protease in Escherichia coli contains protein-activated ATPase and proteolytic functions in distinct subunits. J. Biol. Chem. 263, 8727-8734.
8. Jentoft, N., and Dearborn, D.G. (1979). Labeling of proteins by reductive methylation using cyanoborohydride. J. Biol. Chem. 246, 4359-4365.
9. Kessel, M., Maurizi, M.R., Kim, B., Kocsis, E., Trus, B.L., Singh, S.K., and Stevens, A.C. (1995). Homology in structural organization between E. coli ClpAP protease and the eukaryotic 26S proteasome. J. Mol. Biol. 250, 587-594.
10. 634 for inhibition by maleimides but not catalysis. J. Biol. Chem. 270, 2630-2635.
11. Kroh, H.E., and Simon, L.D. (1990). The ClpP component of Clp protease is the sigma 32-dependent heat shock protein F21.5. J. Bacteriol. 172, 6026-6034.
12. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227, 680-685.
13. Maurizi, M.R. (1992). Proteases and protein degradation in Escherichia coli. Experientia 48, 178-201.
14. Maurizi, M.R., Clark, W.P., Kim, S.-H., and Gottesman, S. (1990). ClpP represents a unique family of serine proteases. J. Biol. Chem. 265, 12546-12552.
15. Seol, J.H., Yoo, S.J., Kim, K.I., Kang, M.S., Ha, D.B., and Chung, C.H. (1994). The 65 kDa protein derived from the internal translational initiation site of the clpA gene inhibits the ATP-dependent protease Ti in Escherichia coli. J. Biol. Chem. 269, 29468-29473.
16. Seol, J.H., Yoo, S.J., Kang, M.S., Ha, D.B., and Chung, C.H. (1995). The 65 KDa protein derived from the internal translational start site of the clpA gene blocks autodegradation of ClpA by the ATP-dependent protease Ti in Escherichia coli. FEBS Lett. 377, 41-43.
17. +-ATPase. Biochim. Biophys. Acta 1194, 329-334.
18. +-pyrophosphatase. J. Biol. Chem. 269, 23342-23350.