Pancreatic lymph nodal and plexus micrometastases detected by enriched polymerase chain reaction and nonradioisotopic single-strand conformation polymorphism analysis: A new predictive factor for recurrent pancreatic carcinoma

Clinical Cancer Research

Volumn 3, Issue 11, 1997, Pages 2143-2149

  Tamagawa, Eiji ^a   Ueda, Masakazu ^a   Takahashi, Shin ^a   Sugano, Kokichi ^b   Uematsu, Shigeto ^a,c   Mukai, Makio ^a   Ogata, Yoshiro ^a,d   Kitajima, Masaki ^a  

^a KEIO UNIVERSITY   (Japan)

^b NATIONAL CANCER CENTER HOSPITAL   (Japan)

^c Imperial Foundation Saiseikai Utsunomiya Hospital   (Japan)

^d TOCHIGI CANCER CENTER   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ADULT; AGED; ARTICLE; CLINICAL ARTICLE; HUMAN; HUMAN TISSUE; LYMPH NODE METASTASIS; MALE; METASTASIS; ONCOGENE K RAS; PANCREAS CARCINOMA; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM;

ADULT; AGED; CARCINOMA, INTRADUCTAL, NONINFILTRATING; CODON; DISEASE-FREE SURVIVAL; DNA, NEOPLASM; FEMALE; GENES, RAS; HUMANS; LYMPHATIC METASTASIS; MALE; MIDDLE AGED; NEOPLASM INVASIVENESS; NEOPLASM METASTASIS; PANCREATIC NEOPLASMS; POINT MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; PREDICTIVE VALUE OF TESTS; RECURRENCE; SURVIVAL ANALYSIS; TREATMENT OUTCOME;

EID: 0030665826     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. American Cancer Society. Cancer Statistics. Atlanta: American Cancer Society, 1994.
2. Statistics and Information Department, Minister's Secretariat. Vital Statistics of Japan, pp. 272-287. Tokyo: Ministry of Health and Welfare, 1992.
3. The Pancreatic Cancer Registration Committee of the Japan Pancreas Society. The Present Study of Pancreatic Cancer Registration. Gann Monograph on Cancer Research no. 43, pp. 107-117. Tokyo: Japanese Cancer Society, 1995.
4. Takahashi, S., Ikeda, N., Tamagawa, E., Tsuzuki, T., Kitajima, M., and Ogata, Y. Recent trends in treatment of TS1 carcinoma of the pancreas. J. Hep. Bil. Pancr. Surgery, 2: 365-370, 1995.
5. James, H. M., Janet, E. P., and William, J. G. Regional chemotherapy for inoperable pancreatic carcinoma. Cancer Suppl., 78: 664-673, 1996.
6. Hayashi, N., Arakawa, H., Nagase, H., Yanagisawa, A., Kato, Y., Ohta, H., Takano, S., Ogawa, M., and Nakamura, Y. Genetic diagnosis identifies occult lymph node metastases undetectable by the histopathological method. Cancer Res., 54: 3853-3856, 1994.
7. Hayashi, N., Ito, I., Yanagisawa, A., Kato, Y., Nakamori, S., Imaoka, S., Watanabe, H., Ogawa, M., and Nakamura, Y. Genetic diagnosis of lymph-node metastasis in colorectal cancer. Lancet, 345: 1257-1259, 1995.
8. Bos, J. L. The ras gene family and human carcinogenesis. Mutat. Res., 195: 255-271, 1988.
9. Bos, J. L. ras oncogene in human cancer: a review. Cancer Res., 49: 4682-4689, 1989.
10. Almoquera, C. S., Forrester, D. K., Martin, J., and Perucho, M. Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes. Cell, 53: 549-554, 1988.
11. Hruban, R. H., van Mansfeld, A. D. M., Offerhaus, G. J. A., van Weering, D. H. J., Allison, D. C., Goodman, S. N., Kensler, T. W., Bose, K. K., Cameron, J. L., and Bos, J. L. K-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization. Am. J. Pathol., 143: 545-554, 1993.
12. Levi, S., Urbano-Ispizua, A., Gill, R., Thomas, D. M., Gilbertson, J., Foster, C., and Marshall, C. J. Multiple K-ras codon 12 mutation on cholangiocarcinomas demonstrated with a sensitive polymerase chain reaction technique, Cancer Res., 57: 3497-3502, 1991.
13. Hasegawa, H., Ueda, M., Watanabe, M., Teramoto, T., Mukai, M., and Kitajima, M. K-ras mutations in early colorectal cancer. . . flat elevated vs polyp-forming cancer. Oncogene, 10: 1413-1416, 1995.
14. Sugano, K., Kyogoku, A., Fukayama, N., Ohkura, H., Shimosato, Y., Sekiya, T., and Hayashi, K. Rapid and simple detection of c-Ki-ras2 gene codon 12 mutations by nonradioisotopic single-strand conformation polymorphism analysis. Lab. Invest., 68: 361-366, 1993.
15. Kondo, H., Sugano, K., Fukayama, N., Kyogoku, A., Nose, H., Shimada, K., Ohkura, H., Ohtsu, A., Yoshida, S., and Shimosato, Y. Detection of point mutations in the K-ras oncogene at codon 12 in pure pancreatic juice for diagnosis of pancreatic carcinoma. Cancer (Phila.), 73: 1589-1594, 1994.
16. Ishikawa, O., Ohigashi, H., Sasaki, Y., Kabuto, T., Furukawa, H., Nakamori, S., Imaoka, S., Imanaga, T., and Kasugai, T. Practical grouping of positive lymph nodes in pancreatic head cancer treated by an extended pancreatectomy. Surgery (St. Louis), 121: 244-249, 1997.
17. Japan Pancreas Society. Classification of Pancreatic Carcinoma. First English Edition. Tokyo: Kanehara & Co., Ltd., 1996.
18. Yamada, H., Yoshida, T., Sakamoto, H., Taira, M., and Sugimura, T. Establishment of a human pancreatic adenocarcinoma cell line (PSN-1) with amplifications of both c-myc and activated c-Ki-ras by a point mutation. Biochem. Biophys. Res. Commun., 140: 167-173, 1986.
19. Goelz, S. E., Hamilton, S. R., and Vogelstein, B. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem. Biophys. Res. Commun., 130: 118-126, 1985.
20. Kitagawa, Y., Ueda, M., Ando, N., Shinozawa, Y. Shimizu, N., and Abe, O. Significance of int-2/hst-1 coamplification as a prognostic factor in patients with esophageal squamous carcinoma. Cancer Res., 57: 1504-1508, 1991.
21. Hasegawa, Y., Takeda, S., Ichii, S., Koizumi, K., Maruyama, M., Fujii, A., Ohta, H., Nakajima, T., Okuda, M., Baba, S., and Nakamura, Y. Detection of K-ras mutations in DNAs isolated from feces of patients with colorectal tumors by mutant-allele-specific amplification (MASA). Oncogene, 10: 1441-1445, 1995.
22. Kahn, S. M. Rapid and sensitive nonradioactive detection of mutant K-ras genes via "enriched" PCR amplification. Oncogene, 6: 1079-1083, 1991.
23. Jacobson, R. D., and Mills, E. N. A highly sensitive assay for mutant ras genes and its application to the study of presentation and relapse genotypes in acute leukemia. Oncogene, 9: 553-563, 1994.
24. Iguchi, H., Sugano, K., Fukayama, N., Ohkura, H., Sadamoto, K., Ohkoshi, K., Seo, Y., Tomoda, H., Funakoshi, A., and Wakasugi, H. Analysis of Ki-ras codon 12 mutations in the duodenal juice of patients with pancreatic cancer. Gastroenterology, 110: 221-226, 1996.
25. Sugano, K., Nakashima, Y., Yamaguchi, K., Fukayama, N., Maekawa, M., Ohkura, H., Kakizoe, T., and Sekiya, T. Sensitive detection of loss of heterozygosity in the TP53 gene in pancreatic adenocarcinoma by fluorescence-based single-strand conformation polymorphism analysis using blunt-end DNA fragments. Genes Chromosomes Cancer, 15: 157-164, 1996.
26. Inoue, T., Nabeshima, K., Kataoka, H., and Koono, M. Feasibility of archival non-buffered formalin-fixed and paraffin-embedded tissues for PCR amplification: an analysis of resected gastric carcinoma. Pathol. Int., 46: 997-1004, 1996.
27. Cameron, J. L., Crist, D. W., Sitzmann, J. V., Hruban, R. H., Boitnott, J. K. Seidler, A. J., and Coleman, J. A. Factors influencing survival after pancreaticoduodenectomy for pancreatic cancer. Am. J. Surg., 161: 120-125, 1991.
28. Takahashi, S., Yoshiro, O., Miyazaki, H., Maeda, D., Murai, S., Yamataka, K., and Tsuzuki, T. Aggressive surgery for pancreatic duct cell cancer: feasibility, validity, limitations. World J. Surg., 19: 653-660, 1995.
29. International Union Against Cancer. TNM Classification of Malignant Tumors, 4th ed. New York: Springer-Verlag, 1987.