Use of degenerate primers and heat-soaked polymerase chain reaction (PCR) to clone a serine protease antigen from Dermatophilus congolensis

Immunology and Cell Biology

Volumn 75, Issue 5, 1997, Pages 484-491

  Mine, Ontiretse M ^a,b   Carnegie, Patrick R ^a  

^a MURDOCH UNIVERSITY   (Australia)

^b BOTSWANA COLLEGE OF AGRICULTURE   (Botswana)

Author keywords

Degenerate primers; Dermatophilosis; Dermatophilus congolensis; Heat soaked; Inosine; Keratinase; Serine protease

Indexed keywords

INOSINE; SERINE PROTEINASE;

ACTINOMYCETACEAE; AMINO ACID SEQUENCE; ANIMAL DISEASE; ANTIGEN DETECTION; ARTICLE; BACILLUS SUBTILIS; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; SEQUENCE HOMOLOGY; SERRATIA MARCESCENS;

ACTINOMYCETACEAE; ANIMALIA; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); DERMATOPHILUS CONGOLENSIS; OVIS ARIES; SERRATIA MARCESCENS;

EID: 0030665107     PISSN: 08189641     EISSN: None     Source Type: Journal    
DOI: 10.1038/icb.1997.75     Document Type: Article

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