Reliable detection of DNA CpG methylation profiles by the isoschizomers MspI/HpaII using oligonucleotide stimulators

BioTechniques

Volumn 23, Issue 5, 1997, Pages 843-847

  Kupper, Dagmar ^a   Reuter, Monika ^a   Meisel, Andreas ^a   Krüger, Detlev H ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; BIOENGINEERING; DNA DETERMINATION; DNA METHYLATION; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE ANALYSIS;

EID: 0030659641     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97235bm17     Document Type: Article

References (9)

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2. Krüger, D.H., D. Kupper, A. Meisel, M. Reuter and C. Schroeder. 1995. The significance of distance and orientation of restriction endonuclease recognition sites in viral DNA genomes. FEMS Microbiol. Rev. 17:177-184.
3. McClelland, M., M. Nelson and E. Raschke. 1994. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucleic Acids Res. 22:3640-3659.
4. Oller, A.R., W. Vanden Broek, M. Conrad and M.D. Topal. 1991. Ability of DNA and spermidine to affect the activity of restriction endonucleases from several bacterial species. Biochemistry 30:2543-2549.
5. Pein, C.-D., M. Reuter, D. Cech and D.H. Krüger. 1989. Oligonucleotide duplexes containing CC(A/T)GG stimulate cleavage of refractory DNA by restriction endonuclease EcoRII. FEBS Lett. 245:141-144.
6. Reuter, M., D. Kupper, C.-D. Pein, M. Petrusyte, V. Siksnys, B. Frey and D.H. Krüger. 1993. Use of specific oligonucleotide duplexes to stimulate cleavage of refractory DNA sites by restriction endonucleases. Anal. Biochem. 209:232-237.
7. Reuter, M., C.-D. Pein, V. Butkus and D.H. Krüger. 1990. An improved method for the detection of Dcm methylation in DNA molecules. Gene 95:161-162.
8. Saluz, H.P. and J.P. Jost. 1993. Major techniques to study DNA methylation, p. 11-26. In J.P. Jost and H.P. Saluz (Eds.), DNA Methylation: Molecular Biology and Biological Significance. Birkhäuser Verlag, Basel.
9. Wentzell, L.M., T.J. Nobbs and S.E. Halford. 1995. The SfiI restriction endonuclease makes a four-strand DNA break at two copies of its recognition sequence. J. Mol. Biol. 248:581-595.