Phosphatidylserine translocation into brain mitochondria: Involvement of a fusogenic protein associated with mitochondrial membranes

Molecular and Cellular Biochemistry

Volumn 175, Issue 1-2, 1997, Pages 71-80

  Camici, Oliva ^a   Corazzi, Lanfranco ^a  

^a UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Brain mitochondria; Fusogenic protein; Glycoprotein; Membrane fusion; Phosphatidylserine decarboxylase; Phosphatidylserine translocation

Indexed keywords

2 ETHOXY 1(2H) QUINOLINECARBOXYLIC ACID ETHYL ESTER; CONCANAVALIN A; FUNCTIONAL GROUP; GLYCOPROTEIN; LIPOSOME; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLSERINE; PRONASE;

ANIMAL TISSUE; ARTICLE; BRAIN MITOCHONDRION; CONTROLLED STUDY; DECARBOXYLATION; MEMBRANE FUSION; MITOCHONDRIAL MEMBRANE; NONHUMAN; PH; PHOSPHOLIPID TRANSFER; PROTEIN PURIFICATION; RAT;

ANIMALS; BRAIN; CARBOXY-LYASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPLASMIC RETICULUM; FLUORESCENT DYES; LIPOSOMES; MEMBRANE FUSION; MEMBRANE GLYCOPROTEINS; MICROSOMES; MITOCHONDRIA; PHOSPHATIDYLSERINES; PRONASE; QUINOLINES; RATS; RATS, WISTAR; RHODAMINES; SPECTROMETRY, FLUORESCENCE;

ANIMALIA;

EID: 0030657705     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006889328983     Document Type: Article

References (38)

1. Voelker DR: Organelle biogenesis and intracellular lipid transport in eukaryotes. Microb Rev 55: 543-560,1991
2. Butler M, Morell P: The role of phosphatidylserine decarboxylase in brain phospholipid metabolism. J Neurochem 41: 1445-1454,1983
3. 14C]serine in isolated rat hepatocytes. Biochim Biophys Acta 833: 396-405,1985
4. Rusiñol AE, Cui Z, Chen MH, Vance JE: A unique mitochondria-associated membrane fraction from rat liver has a high capacity for lipid synthesis and contains pre-Golgi secretory proteins including nascent lipoproteins. J Biol Chem 269: 27494-27502,1994
5. Katz J, Wals PA, Golden S, Raijman L: Mitochondrial-reticular cytostructure in liver cells. Biochem J 214: 795-813,1983
6. Cascarano J, Montisano DF, Pickett CB, James TW: Rough endoplasmic reticulum-mitochondrial complexes from rat liver. An extramitochondrial succinic dehydrogenase associated with this rough endoplasmic reticulum. Exp Cell Res 139: 39-50,1982
7. Camici O, Corazzi L: Import of phosphatidylethanolamine for the assembly of rat brain mitochondrial membranes. J Membr Biol 148: 169-176,1995
8. Voelker DR: Reconstitution of phosphatidylserine import into rat liver mitochondria. J Biol Chem 264: 8019-8025,1989
9. Corazzi L, Pistolesi R, Carlini E, Arienti G: Transport of phosphatidylserine from microsomes to the inner mitochondrial membrane in brain tissue. J Neurochem 60: 50-56,1993
10. Vance JE: Newly made phosphatidylserine and phosphatidylethanolamine are preferentially translocated between rat liver mitochondria and endoplasmic reticulum. J Biol Chem 266: 89-97,1991
11. Hovius R, Faber B, Brigot B, Nicolay K, de Kruijff B : On the mechanism of the mitochondrial decarboxylation of phosphatidylserine. J Biol Chem 267: 16790-16795,1992
12. Carlini E, Corazzi L, Camici O, Arienti G: The fate of phosphatidylethanolamine formed by decarboxylation in rat brain mitochondria. Biochem Molec Biol Intern 29: 821-829,1993
13. Shiao YJ, Lupo G, Vance JE: Evidence that phosphatidylserine is imported into mitochondria via a mitochondria-associated membrane and that the majority of mitochondrial phosphatidylethanolamine is derived from decarboxylation of phosphatidylserine. J Biol Chem 270: 11190-11198,1995
14. Wilschut J, Düzgünes N, Fraley R, Papahadjopoulus D: Studies on the mechanism of membrane fusion: kinetics of calcium ion induced fusion of phosphatidylserine vesicles followed by a new assay for mixing of aqueous contents. Biochemistry 19: 6011-6021,1980
15. Rakowska M, Zborowski J, Corazzi L: A fusogenic protein from rat brain microsomal membranes: partial purification and reconstitution into liposomes. J Membr Biol 142: 35-42,1994
16. Hoekstra D, de Boer T, Klappe K, Wilschut J. Fluorescence method for measuring the kinetics of fusion between biological membranes. Biochemistry 23: 5675-5681,1984
17. Corazzi L, Pistolesi R, Arienti G: The fusion of liposomes to rat brain microsomal membranes regulates phosphatidylserine synthesis. J Neurochem 56: 207-212,1991
18. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685,1970
19. Giulian GG, Moss RL, Greaser M: Improved methodology for analysis and quantitation of proteins in one-dimensional silver-stained slab gels. Anal Biochem 129: 277-287,1983
20. Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354,1979
21. Folch J, Lees M, Sloane Stanley GH: A simplified method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226: 497-509,1957
22. Rouser, G, Kritchevsky, G, Yamamoto, A: Column Chromatographic and associated procedures for separation and determination of phosphatides and glycolipids. In: GV Marinetti (ed). Lipid Chromatographic Analysis, vol. 1, 99-162, Marcel Dekker, Inc., New York,1967
23. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254,1976
24. Bartlett GR: Phosphorus assay in column chromatography. J Biol Chem 234: 466-468,1959
25. Harb J, Meflah K, di Pietro A, Bernard S, Gautheron DC: Chemical modification of essential carboxyl group and histidine residue in the plasma 5′-nucleotidase. Biochim Biophys Acta 870: 320-326,1986
26. Jasinska R, Zborowski J, Somerharju P: Intramitochondrial distribution and transport of phosphatidylserine and its decarboxylation product, phosphatidylethanolamine. Application of pyrene-labeled species. Biochim Biophys Acta 1152: 161-170,1993
27. Percy AK, Moore JF, Carson MA, Waechter CJ: Characterization of brain phosphatidylserine decarboxylase: localization in the mitochondrial inner membrane. Arch Biochem Biophys 223: 484-494,1983
28. Vance JE: Phospholipid synthesis in a membrane fraction associated with mitochondria. J Biol Chem 265: 7248-7256,1990
29. Ardail D, Gasnier F, Lerme F, Simonot C, Louisot P, Gateau-Roesch O: Involvement of mitochondrial contact sites in the subcellular compartmentalization of phospholipid biosynthetic enzymes. J Biol Chem 268: 25985-25992,1993
30. Schneider H, Lemasters JJ, Höchli M, Hackenbrock CR: Fusion of liposomes with mitochondrial inner membranes. Proc Natl Acad Sci USA 77: 442-446,1980
31. Murata M, Takahashi S, Kagiwada S, Suzuki A, Ohnishi S: pH-dependent membrane fusion and vesiculation of phospholipid large unilamellar vesicles induced by amphiphilic anionic and cationic peptides. Biochemistry 31: 1986-1992,1992
32. Puri A, Grimaldi S, Blumenthal R: Role of viral envelope sialic acid in membrane fusion mediated by the vesicular stomatitis virus envelope glycoprotein. Biochemistry 31: 10108-10113,1992
33. Levrat C, Ardail D, Louisot P: Comparative study of the N-glycoprotein synthesis through dolichol intermediates in mitochondria, Golgi apparatus-rich fraction and endoplasmic reticulum-rich fraction. Int J Biochem 22: 287-293,1990
34. Gasnier F, Louisot P, Gateau O: Galactosyltransferase activities in mitochondria outer membrane: biosynthesis of galactosylated proteins. Int J Biochem 21: 173-181,1989
35. Jacob WA, Bakker A, Hertsens RC, Biermans W: Mitochondrial matrix granules: their behavior during changing metabolic situations and their relationship to contact sites between inner and outer mitochondrial membranes. Micros Res Tech 27: 307-318,1994
36. Mandal DK, Brewer CF: Interactions of Concanavalin A with glycoproteins: formation of homogeneous glycoprotein-lectin cross-linked complexes in mixed precipitation systems. Biochemistry 31: 12602-12609,1992
37. Fini C, Camici M, Minelli A, Floridi A, Ipata PL: Concanavalin A induced inhibition of 5′-nucleotidase from guinea pig skeletal muscle and bull seminal plasma: a comparative study. Int J Biochem 8: 683-689,1986
38. Gaigg B, Simbeni R, Hrastnik C, Paltauf F, Daum G: Characterization of a microsomal subfraction associated with mitochondria of the yeast, Saccharomyces cerevisiae. Involvement in synthesis and import of phospholipids into mitochondria. Biochim Biophys Acta 1234: 214-220,1995