Purified metallo-protease from the pathogenic haemoflagellate Cryptobia salmositica and its in vitro proteolytic activities

Diseases of Aquatic Organisms

Volumn 30, Issue 3, 1997, Pages 177-185

  Zuo, X ^a   Woo, P T K ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Cryptobia salmositica; Hydrolysis; Metallo protease; Proteins; Purification

Indexed keywords

CRYPTOBIA SALMOSITICA; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 0030656307     PISSN: 01775103     EISSN: None     Source Type: Journal    
DOI: 10.3354/dao030177     Document Type: Article

References (35)

1. Arnesen JA, Eggset G, Jørgensen TO (1995) Partial purification and characterization of extracellular metalloproteases from Aeromonas salmonicida spp. J Fish Dis 18:283-295
2. Bahmanrokh M, Woo PTK (1994) The histopathology of cryptobiosis in juvenile Oncorhynchus mykiss (Walbaum). In: Özcel MA, Alkan MZ (eds) Eighth International Congress of Parasitology, Abstracts, Vol II. Turkish Society for Parasitology, Izmir, p 434
3. Bonaldo MC, D'Escoffier LN, Salles JM, Goldenberg S (1991) Characterization and expression of proteases during Trypanosoma cruzi metacyclogenesis. Expl Parasitol 73:44-51
4. Bower SM, Margolis L (1983) Direct transmission of the haemoflagellate Cryptobia salmositica among Pacific salmon (Oncorhynchus spp.). Can J Zool 61:1242-1250
5. Bower SM, Woo PTK (1977) Morphology and host specificity of Cryptobia catostomi n.sp. (Protozoa: Kinetoplastida) from white sucker (Catostomus commersoni) in southern Ontario. Can J Zool 55:1082-1092
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
7. Gallop PM, Paz MA (1975) Posttranslational protein modifications with special attention to collagen and elastin. Physiol Rev 55:418-487
8. Greig S, Ashall F (1990) Electrophoretic detection of Trypanosoma cruzi peptidases. Mol Biochem Parasitol 39:31-38
9. Hames BD (1981) An introduction to polyacrylamide gel electrophoresis. In: Hames BD, Rickwood D (eds) Gel electrophoresis of proteins: a practical approach. IRL Press, London, p 1-91
10. Knox DP, Redmond DL, Jones DG (1993) Characterization of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode. Parasitology 106:395-404
11. Kohnert U, Oberhoff R, Fedrowitz J, Bergmann U, Rauterberg J, Tscheche H (1988) The degradation of Collagen by a metalloprotease from human leucocytes. In: Horl WH, Heidland A (eds) Proteases II: potential role in health and disease. Plenum Press, New York, p 33-44
12. Li S, Woo PTK (1991) Anorexia reduces the severity of cryptobiosis in Oncorhynchus mykiss. J Parasitol 77:467-471
13. McKerrow JH, Sun E, Rosenthal PJ, Bouvier J (1993) The proteases and pathogenicity of parasitic protozoa. Annu Rev Microbiol 47:821-853
14. Munoz ML, Calderon J, Rojkind M (1982) The collagenase of Entamoeba histolytica. J Exp Med 155:42-51
15. Munoz ML, Lamoyi E, Leon G, Tovar R, Perez-Garcia J (1990) Antigens in electron-dense granules from Entamoeba histolytica as possible markers for pathogenicity. J Clin Microbiol 28:2418-2424
16. Norqvist A, Norrman B, Wolf-Watz H (1990) Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguillarum. Infect Immun 58:3731-3736
17. Piras MM, Henriquez D, Piras R, Nigri S (1985) The effect of proteolytic enzymes and protease inhibitors on the interaction T. cruzi-fibroblasts. Mol Biochem Parasitol 14: 151-163
18. Schulte W, Scholze H (1989) Action of the major protease from Entamoeba histolytica on proteins of the extracellular matrix. J Protozool 36:538-543
19. Thomas PT, Woo PTK (1988) Cryptobia salmositica: an in vitro study on the mechanism of anaemia in infected rainbow trout, Salmo gairdneri Richardson. J Fish Dis 11:425-431
20. Thomas PT, Woo PTK (1989) An in vitro study on the haemolytic components from Cryptobia salmositica (Sarcomastigophora: Kinetoplastida). J Fish Dis 12:389-393
21. Thomas PT, Woo PTK (1992) In vitro culture and multiplication of Cryptobia catostomi and experimental infection in white sucker (Catostomus commersoni). Can J Zool 70: 201-204
22. Woo PTK (1978) The division process of Cryptobia salmositica in experimentally infected rainbow trout (Salmo gairdneri). Can J Zool 56:1514-1518
23. Woo PTK (1979) Trypanoplasma salmositica: experimental infections in rainbow trout, Salmo gairdneri. Expl Parasitol 47:36-48
24. Woo PTK (1987) Cryptobia and cryptobiosis in fishes. In: Baker JR, Muller R (eds) Advances in parasitology, Vol 26. Academic Press, London, p 199-237
25. Woo PTK (1994) Flagellate parasites of fish. In: Kreier JP (ed) Parasitic protozoa, Vol 8. Academic Press, New York, p 1-80
26. Woo PTK, Li S (1990) In vitro attenuation of Cryptobia salmositica and its use as a live vaccine against cryptobiosis in Oncorhynchus mykiss. J Parasitol 76:752-755
27. Woo PTK, Poynton SL (1995) Diplomonadida, Kinetoplastida and Amoebida (Phylum Sarcomastigophora). In: Woo PTK (ed) Fish diseases and disorders, Vol 1, Protozoan and metazoan infections. CAB International, Wallingford, p 27-96
28. Woo PTK, Thomas PT (1991) Polypeptide and antigen profiles of Cryptobia salmositica, C. bullocki and C. catostomi (Sarcomastigophora: Kinetoplastida) isolated from fishes. Dis Aquat Org 11:201-205
29. Woo PTK, Thomas PT (1992) Comparative in vitro studies on virulent and avirulent strains of Cryptobia salmositica (Sarcomastigophora: Kinetoplastida). J Fish Dis 15:261-266
30. Woo PTK, Wehnert SD (1983) Direct transmission of a haemoflagellate, Cryptobia salmositica (Kinetoplastida: Bodonia) between rainbow trout under laboratory conditions. J Protozool 30:334-337
31. Yu WJ, Greenwood JS (1994) Purification and characterization of a cysteine proteinase involved in globulin hydrolysation in germinated Vicia faba L. J Exp Bot 45:261-268
32. Zuo X, Woo PTK (1996) Acid phosphatase in the pathogenic and nonpathogenic haemoflagellates, Cryptobia spp., of fishes. J Parasitol 82:893-899
33. Zuo X, Woo PTK (1997a) Proteases in pathogenic and non-pathogenic haemoflagellates, Cryptobia spp. (Sarcomastigophora: Kinetoplastida), of fishes. Dis Aquat Org 29: 57-65
34. Zuo X, Woo PTK (1997b) Natural antiproteases in rainbow trout, Oncorhynchus mykiss and brook charr, Salvelinus fontinalis and the in vitro neutralization of fish α2-macroglobulin by the metalloprotease from the pathogenic haemoflagellate, Cryptobia salmositica. Parasitology 114:375-381
35. Zuo X, Woo PTK (1997c) In vivo neutralization of Cryptobia salmositica metallo-protease by α2-macroglobulin in the blood of rainbow trout Oncorhynchus mykiss and brook charr Salvelinus fontinalis. Dis Aquat Org 29:67-72