Site-Directed Mutagenesis of the Phosphocholine-Binding Site of Human C-Reactive Protein: Role of Thr76 and Trp67

Journal of Immunology

Volumn 158, Issue 1, 1997, Pages 345-350

  Agrawal, Alok ^a   Lee, Sungjoong ^a   Carson, Mike ^b   Narayana, S V L ^b   Greenhough, Trevor J ^c   Volanakis, John E ^a  

^a The University of Alabama at Birmingham   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c KEELE UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

C REACTIVE PROTEIN; PHOSPHORYLCHOLINE; SERUM AMYLOID P; THREONINE; TRYPTOPHAN;

ARTICLE; CHEMICAL STRUCTURE; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS;

C-REACTIVE PROTEIN; HUMANS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLCHOLINE; PROTEIN BINDING; SERUM AMYLOID P-COMPONENT; THREONINE; TRYPTOPHAN;

EID: 0030641806     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Gotschlich, E. C., and G. M. Edelman. 1965. C-reactive protein: a molecule composed of subunits. Proc. Natl. Acad. Sci. USA 54:558.
2. Osmand, A. P., B. Friedenson, H. Gewurz, R. H. Painter, T. Hofmann, and E. Shelton. 1977. Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins). Proc. Natl. Acad. Sci. USA 74:739.
3. Tillett, W. S., and T. Francis, Jr. 1930. Serological reactions in pneumonia with a non-protein somatic fraction of pneumococcus. J. Exp. Med. 52:561.
4. Volanakis, J. E., and M. H. Kaplan. 1971. Specificity of C-reactive protein for choline phosphate residues of pneumococcal C-polysaccharide. Proc. Soc. Exp. Biol. Med. 136:612.
5. Volanakis, J. E., and K. W. A. Wirtz. 1979. Interaction of C-reactive protein with artificial phosphatidylcholine bilayers. Nature 281:155.
6. DuClos, T. W., L. T. Zlock, and R. L. Rubin. 1988. Analysis of the binding of C-reactive protein to histones and chromatin. J. Immunol. 141:4266.
7. Agrawal, A., J. M. Kilpatrick, and J. E. Volanakis. 1993. Structure and function of human C-reactive protein. In Acute Phase Proteins: Molecular Biology, Biochemistry and Clinical Applications. A. Mackiewicz, I. Kushner, and H. Baumann, eds. CRC Press, Boca Raton, p. 79.
8. Hind, C. R. K., P. M. Collins, M. L. Baltz, and M. B. Pepys. 1985. Human serum amyloid P component, a circulating lectin with specificity for the cyclic 4,6-pyruvate acetal of galactose. Biochem. J. 225:107.
9. Schwalbe, R. A., B. Dahlbäck, J. E. Coe, and G. L. Nelsestuen. 1992. Pentraxin family of proteins interact specifically with phosphorylcholine and/or phosphorylethanolamine. Biochemistry 31:4907.
10. Agrawal, A., Y. Xu, D. Ansardi, K. J. Macon, and J. E. Volanakis. 1992. Probing the phosphocholine-binding site of human C-reactive protein by site-directed mutagenesis. J. Biol. Chem. 267:25352.
11. Emsley, J., H. E. White, B. P. O'Hara, G. Oliva, N. Srinivasan, I. J. Tickle, T. L. Blundell, M. B. Pepys, and S. P. Wood. 1994. Structure of pentameric human serum amyloid P component. Nature. 367:338.
12. Shrive, A. K., G. M. T. Cheetham, D. Holden, D. A. A. Myles, W. G. Turnell. J. E. Volanakis, M. B. Pepys, A. C. Bloomer, and T. J. Greenhough. 1996. Three-dimensional structure of human C-reactive protein. Nature Struct. Biol. 3:346.
13. Volanakis, J. E., Y. Xu, and K. J. Macon. 1990. Human C-reactive protein and host defense. In Defense Molecules. J. J. Marchalonis and C. L. Reinish, eds. Alan R. Liss, Inc., p. 161.
14. Zoller, M. J., and M. Smith. 1983. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol. 100:468.
15. Tabor, S., and C. C. Richardson. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl. Acad. Sci. USA 84:4767.
16. Wong, G. G., J. S. Witek, P. A. Temple, K. M. Wilkens, A. C. Leary, D. P. Luxenberg, S. S. Jones, E. L. Brown, R. M. Kay, E. C. Orr, C. Shoemaker, D. W. Golde, R. J. Kaufman, R. M. Hewick, E. A. Wang, and S. C. Clark. 1985. Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science 228:810.
17. Volanakis, J. E., W. L. Clements, and R. E. Schrohenloher. 1978. C-reactive protein: purification by affinity chromatography and physicochemical characterization. J. Immunol. Methods 23:285.
18. Liu, T.-Y., and E. C. Gotschlich. 1963. The chemical composition of pneumococcal C-polysaccharide. J. Biol. Chem. 238:1928.
19. Kilpatrick, J. M., J. F. Kearney, and J. E. Volanakis. 1982. Demonstration of calcium-induced conformational change(s) in C-reactive protein by using monoclonal antibodies. Mol. Immunol. 19:1159.
20. Agrawal, A., and J. E. Volanakis. 1994. Probing the C1q-binding site on human C-reactive protein by site-directed mutagenesis. J. Immunol. 152:5404.
21. Jones, T. A. 1978. A graphic model building and refinement system for macromolecules. J. Appl. Cryst. 11:268.
22. 2O. J. Am. Chem. Soc. 100:4453.
23. Brünger, A. T. 1992. X-PLOR Version 3.1: A System for X-Ray Crystallography and NMR. Yale University Press, New Haven.
24. Carson, M. 1987. Ribbon models of macromolecules. J. Mol. Graphics 5:103.
25. Jennings, H. J., C. Lugowski, and N. M. Young. 1980. Structure of the complex polysaccharide C-substance from Streptococcus pneumoniae type 1. Biochemistry 19:4712.
26. Higginbotham, J. D., M. Heidelberger, and E. C. Gotschlich. 1970. Degradation of a pneumococcal type-specific polysaccharide with exposure of group specificity. Proc. Natl. Acad. Sci. USA 67:138.
27. Young, N. M., and R. E. Williams. 1978. Comparison of the secondary structures and binding sites of C-reactive protein and the phosphorylcholine-binding murine myeloma proteins. J. Immunol. 121:1893.
28. Dong, A., W. S. Caughey, and T. W. DuClos. 1994. Effects of calcium, magnesium and phosphorylcholine on secondary structures of human C-reactive protein and serum amyloid P component observed by infrared spectroscopy. J. Biol. Chem. 269:6424.