Purification and some properties of a novel dsRNA degrading nuclease bound to rye germ ribosomes

Acta Biochimica Polonica

Volumn 44, Issue 1, 1997, Pages 61-68

  Siwecka, Maria A ^a  

^a INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

Author keywords

Double stranded RNA; Germ; Nuclease; Ribosome; Secale cereale L.

Indexed keywords

PENICILLIUM; PENICILLIUM CHRYSOGENUM; SECALE CEREALE;

DOUBLE STRANDED RNA; RIBONUCLEASE; VEGETABLE PROTEIN; VIRUS RNA;

ARTICLE; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PENICILLIUM CHRYSOGENUM; RIBOSOME; RYE; VIROLOGY;

PENICILLIUM CHRYSOGENUM; PLANT PROTEINS; RIBONUCLEASES; RIBOSOMES; RNA, DOUBLE-STRANDED; RNA, VIRAL; SECALE CEREALE;

EID: 0030629874     PISSN: 0001527X     EISSN: None     Source Type: Journal    
DOI: 10.18388/abp.1997_4440     Document Type: Article

References (18)

1. Bielka, H. (1982) Proteolytic and nucleolytic activities of ribosomal particles; in Eucaryotic ribosome (Bielka, H. ed.) pp. 98-99, Academic Verlag , Berlin.
2. Wilson, D.M. III, Deutsch, W.A. & Kelley, M.R. (1994) Drosophila ribosomal protein S3 contains an activity that cleaves DNA at apurinic/apyrimidinic sites. J. Biol. Chem. 269, 25359-25364.
3. Siwecka, M.A., Golaszewski, T. & Szarkowski, J.W. (1977) Association of ribonucleolytic activity with rye embryo ribosomes. Bull. Acad. Polon. Sci. Ser. Sci. Biol. 25, 15-19.
4. Siwecka, M.A., Rytel, M. & Szarkowski, J.W. (1979) The presence of deoxyribonucleolytic activity in cytoplasmic ribosomes of rye (Secale cereale L.) germs. Acta Biochim. Polon. 26, 97-101.
5. Siwecka, M.A., Rytel, M. & Szarkowski, J.W. (1982) Effect of rye embryo ribosome nuclease on double-stranded RNA. Phytochemistry 21, 273-375.
6. Kuligowska, E. Klarkowska, D. & Szarkowski, J.W. (1976) Alkaline ribonuclease from rye germ cytosol. Acta Biochim. Polon. 23, 115-126.
7. Kuligowska, E., Klarkowska D. & Szarkowski, J.W. (1980) An acid ribonuclease from rye germ cytosol. Phytochemistry 19, 31-35.
8. Siwecka, M.A., Rytel, M. & Szarkowski, J.W. (1989) Purification and characterization of nuclease I associated with rye germ ribosomes. Acta Biochim. Polon. 36, 45-62.
9. Siwecka, M.A., Rytel, M. & Szarkowski, J.W. (1988) Ribosomal nuclease with activity towards double-stranded RNA; in Metabolism and Enzymology of Nucleic Acids, Including Gene Manipulations (Zelinka, J. & Balan, J., eds.) pp. 245-250, Plenum Press, New York, London.
10. Siwecka, M.A., Rytel, M. & Szarkowski, J.W. (1983) Ionic control of ribonucleolytic activities associated with rye embryo ribosomes. Acta Physiol. Plant. 5, 105-111.
11. Matoušek, J., Trnena, L., Oberhauser, R., Lichtenstein, C.P. & Nellen, W. (1994) dsRNA degrading nucleases are differentially expressed in tobacco anthers. Biol. Chem. Hoppe-Seyler 375, 261-269.
12. Wilson, C.M. (1982) Plant nucleases: biochemistry and development of multiple molecular forms; in Isozymes: Current Topics in Biological and Medical Research; vol. 6, pp. 33-54, A.R. Liss Inc. New York.
13. Anfinsen, C.B., Redfield, R.R., Choate, W.L., Page, J. & Carrol, W.R. (1954) Studies of the gross structure, cross-linkages and terminal sequences in ribonuclease. J. Biol. Chem. 207, 201-210.
14. Bradford, M.M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal. Biochem. 72, 248-254.
15. Petersen, G.L. (1979) Review of the Folin protein quantitation method of Lowry, Rosebrough, Farr and Randall. Anal. Biochem. 100, 201-220.
16. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of heads of bacteriophage T4. Nature 227, 681-685.
17. Brawerman, G. (1993) mRNA degradation in eukaryotic cells: an overview; in Control of Messenger RNA Stability; (Belasco, J. & Brawerman, G. ed.) p. 157, Academic Press Inc. London.
18. D'Alessio, Di Donato, A.D., Parente, A. & Picolli, R. (1991) Seminal RNAse: A unique member of the ribonuclease superfamily. Trends Biochem. Sci. 16, 104-106.