Detection of an antigenic protein of Leptospira interrogans which shares epitopes with the equine cornea and lens

Veterinary Journal

Volumn 153, Issue 1, 1997, Pages 75-79

  Parma, A E ^a   Sanz, M E ^a   Lucchesi, P M ^a   Mazzonelli, J ^a   Petruccelli, M A ^b  

^a UNIVERSIDAD NACIONAL DEL CENTRO DE LA PROVINCIA DE BUENOS AIRES   (Argentina)

^b UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

Author keywords

Antigen; Cornea; Equine; Immunogold; Leptospira

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL PROTEIN; EPITOPE;

ANIMAL; ANIMAL DISEASE; ARTICLE; CORNEA; CROSS REACTION; ELECTRON MICROSCOPY; HORSE; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; LENS; LEPTOSPIRA INTERROGANS; POLYACRYLAMIDE GEL ELECTROPHORESIS; RABBIT; ULTRASTRUCTURE; WESTERN BLOTTING;

ANIMALS; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BLOTTING, WESTERN; CORNEA; CROSS REACTIONS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPES; HORSES; IMMUNOHISTOCHEMISTRY; LENS, CRYSTALLINE; LEPTOSPIRA INTERROGANS; MICROSCOPY, ELECTRON; RABBITS;

EID: 0030624198     PISSN: 10900233     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1090-0233(97)80011-1     Document Type: Article

References (19)

1. BLOGG, J. R. & COLES, E. H. (1970). Aqueous humor proteins: a review. Veterinary Bulletin, 40, 347-51.
2. COOK, C. S., PFEIFFER, R. L. & HARLING, D. E. (1983). Equine recurrent uveitis. Equine Ophthalmology, 2, 57-60.
3. DOHERTY, J. P., ADLER, B., ROOD, J. I., BILLINGTON, S. J. & FAINE, S. (1989). Expression of two conserved leptospiral antigens in Escherichia coli. Journal Medical Microbiology 28, 143-49.
4. ELLINGHAUSEN, H. C. JR. & MCCULLOUGH, W. C. (1965). Nutrition of Leptospira pomona and growth of 13 other serotypes: a serum-free medium employing oleic albumin complex. American Journal Veterinary Research 26, 39-44.
5. GRAVEKAMP, C., VAN DE KEMP, H., FRANZEN, M. et al. (1993). Detection of seven species of pathogenic leptospires by PCR using two sets of primers. Journal of General Microbiology 139, 1691-1700.
6. HAAKE, D. A., CHAMPION, C. I., MARTINICH, C. et al. (1993). Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira spp. Journal Bacteriology 175, 4225-34.
7. HOVIND-HOUGEN, K. (1986). Morphology of leptospires. Electron microscopic studies in relation to the classification of Leptospira. In The Present State of Leptospirosis Diagnosis and Control, pp. 1-12, eds W. A. Ellis & T. W. Little. Dordrecht: Martinus Nijhoff Publishers.
8. LAEMMLI, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-85.
9. NUNES-EDWARDS, P. L., THIERMANN, A. B., BASSFORD, P. J. JR & STAMM, L. V. (1985). Identification and characterization of the protein antigens of Leptospira interrogans serovar hardjo. Infection and Immunity, 48, 492-97.
10. PARMA, A. E., CERONE, S. & SANSINANEA, S. (1992a). Biochemical analysis SDS-PAGE and Western blotting of the antigenic relationship between Leptospira and equine ocular tissues. Veterinary Immunology and Immunopathology 33, 179-85.
11. PARMA, A. E., CERONE, S., SANSINANEA, S. & GHEZZI, M. (1992b). C3 fixed in vivo to cornea from horses inoculated with Leptospira interrogans. Veterinary Immunology and Immunopathology 34, 181-87.
12. PARMA, A. E., FERNANDEZ, A., SANTISTEBAN, C., BOWDEN, R. & CERONE, S. (1987). Tears and aqueous humor from horses inoculated with Leptospira contain antibodies which bind to cornea. Veterinary Immunology and Immunopathology 14, 181-85.
13. PARMA, A. E., SANTISTEBAN, C., FERNANDEZ, A., CERONE, S. & BOWDEN, R. (1986). Antigenic relationship between Leptospira interrogans, equine crystalline and cornea tested by ELISA. Revista de Medicina Veterinaria 67, 72-76.
14. PARMA, A. E., SANTISTEBAN, C., VILLALBA, J. & BOWDEN, R. (1985). Experimental demonstration of an antigenic relationship between Leptospira and equine cornea. Veterinary Immunology and Immunopathology 10, 215-24.
15. SAS INSTITUTE INC. (1989) SAS/STAT® User's Guide, Version 6, Fourth Edition, Vol. 2, pp. 846, de. SAS Institute Inc., Cary, NC, USA: SAS Institute Inc.
16. TOWBIN, H., STAEHELIN, T. & GORDON, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences USA 76, 4350-54.
17. TSAI, C. M. & FRASCH, E. (1982). A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Analytical Biochemistry, 119, 115-19.
18. WORLD HEALTH ORGANIZATION. (1965). Classification of leptospires and recent advance in leptospirosis. Bulletin of the World Health Organization, 32, 881-91.
19. YAMAGUCHI, T., ONO, E. & YANAGAWA, R. (1988). Expression of antigen genes of Leptospira interrogans serovar canicola in Escherichia coli. Microbiology Immunology 32, 1179-87.