Rainbow(TM) Universal CPG: A versatile solid support for oligonucleotide synthesis

BioTechniques

Volumn 22, Issue 4, 1997, Pages 752-756

  Nelson, P S ^a   Muthini, S ^a   Vierra, M ^a   Acosta, L ^a   Smith, T H ^a  

^a CLONTECH Laboratories Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE; BIOTIN; COMPLEMENTARY DNA; GLASS; LITHIUM CHLORIDE; OLIGODEOXYRIBONUCLEOTIDE;

ARTICLE; AUTOMATION; NUCLEOTIDE METABOLISM; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; CHEMISTRY; DNA TEMPLATE; HUMAN; ISOLATION AND PURIFICATION; PLACENTA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SYNTHESIS; TEMPERATURE;

AUTOMATION; BIOTIN; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLASS; HUMANS; LITHIUM CHLORIDE; OLIGODEOXYRIBONUCLEOTIDES; PLACENTA; POLYMERASE CHAIN REACTION; TEMPERATURE; TEMPLATES, GENETIC;

EID: 0030615269     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97224pf01     Document Type: Article

References (8)

1. Anonymous. 1991. Quick Deprotection of Oligodeoxyribonucleotides Synthesized Using Conventional Phosphoramidites. Technical Bulletin No. 041. Cruachem, Inc., Herndon, VA.
2. Beaucage, S. 1993. Oligodeoxyribonucleotide synthesis. In S. Agrawal (Ed.), Methods in Molecular Biology, Vol 20: Protocols for Olignucleotides and Analogs. Humana Press, Totowa, NJ.
3. deBear, J.S., J. Hayes, M. Koleck and G. Gough. 1987. A universal glass support for oligodeoxyribonucleotide synthesis. Nucleosides Nucleotides 6:821-830.
4. Gait, M.J. (Ed.). 1984. Oligodeoxyribonucleotide Synthesis: A Practical Approach. IRL Press, Washington, DC.
5. Hardy, P., D. Holland, S. Scott, A. Garman, C. Newton and M. McLean. 1994. Reagents for the preparation of two oligodeoxyribonucleotides per synthesis (TOPS™). Nucleic Acids Res. 22:2998-3004.
6. Hudson, D. 1988. Methodological implications of simultaneous solid-phase peptide synthesis. 1. Comparison of different coupling procedures. J. Org. Chem. 53:617.
7. Smith, T.H., M.A. Kent, S. Muthini, S. Boone and P.S. Nelson. 1996. Oligodeoxyribonucleotide labeling methods 4. Direct labeling reagents with a novel, non-nucleosidic, chirally defined 2-deoxy-β-D-ribosyl backbone. Nucleosides Nucleotides 15:1581-1594.
8. Yu, C.J. 1995. A universal support and modified ammonia deprotection for synthesis of oligodeoxyribonucleotide. Abstract No. 51. A.A.C.C. Nucleic Acids Conference, San Diego, CA.