The stpA gene from Synechocystis sp. strain PCC 6803 encodes the glucosylglycerol-phosphate phosphatase involved in cyanobacterial osmotic response to salt shock

Journal of Bacteriology

Volumn 179, Issue 5, 1997, Pages 1727-1733

  Hagemann, Martin ^a   Schoor, Arne ^a   Jeanjean, Robert ^b   Zuther, Ellen ^a   Joset, Françoise ^b  

^a UNIVERSITY OF ROSTOCK   (Germany)

^b LCB CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; BACTERIAL RNA; GLUCOSE PHOSPHATE; GLYCEROPHOSPHATE; MESSENGER RNA; PHOSPHATASE; SODIUM CHLORIDE; GLUCOSIDE; GLUCOSYLGLYCEROL; GLUCOSYLGLYCEROL PHOSPHATE PHOSPHATASE; GLUCOSYLGLYCEROL-PHOSPHATE PHOSPHATASE; HYBRID PROTEIN;

ADAPTATION; ANTIBODY PRODUCTION; ARTICLE; BACTERIUM MUTANT; CYANOBACTERIUM; ENZYME ACTIVITY; IMMUNOBLOTTING; NONHUMAN; OSMOREGULATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; STRAIN DIFFERENCE; AMINO ACID SEQUENCE; BACTERIAL GENE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; NORTHERN BLOTTING; OSMOTIC PRESSURE; PHYSIOLOGY; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); CYANOBACTERIA; ESCHERICHIA COLI; SYNECHOCYSTIS; SYNECHOCYSTIS SP.;

AMINO ACID SEQUENCE; BLOTTING, NORTHERN; BLOTTING, WESTERN; CLONING, MOLECULAR; CYANOBACTERIA; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GLUCOSIDES; MOLECULAR SEQUENCE DATA; MUTATION; OSMOTIC PRESSURE; PHOSPHORIC MONOESTER HYDROLASES; RECOMBINANT FUSION PROTEINS; SODIUM CHLORIDE;

EID: 0030614762     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.5.1727-1733.1997     Document Type: Article

References (30)

1. Apte, S. R., and R. Haselkorn. 1990. Cloning of salinity stress-induced genes from the salt-tolerant nitrogen fixing cyanobacterium Anabaena torulosa. Plant Mol. Biol. 15:723-733.
2. Borowitzka, L. J., S. Demmerle, M. A. Mackay, and R. S. Norton. 1980. Carbon-13 nuclear magnetic resonance study of osmoregulation in a blue-green alga. Science 210:650-651.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
5. Erdmann, N. 1983. Organic osmoregulatory solutes in blue-green algae. Z. Pflanzenphysiol. 110:147-155.
6. Giæver, H. M., O. B. Styrvold, I. Kaasen, and A. R. Strom. 1988. Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli. J. Bacteriol. 170:2841-2849.
7. Hagemann, M., and E. Zuther. 1992. Selection and characterization of mutants of the cyanobacterium Synechocystis sp. PCC 6803 unable to tolerate high salt concentrations. Arch. Microbiol. 158:429-434.
8. Hagemann, M., and N. Erdmann. 1994. Activation and pathway of glucosylglycerol biosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Microbiology 140:1427-1431.
9. Hagemann, M., A. Schoor, and N. Erdmann. 1996. NaCl acts as a direct modulator in the salt adaptive response: salt-dependent activation of glucosylglycerol synthesis in vivo and in vitro. J. Plant Physiol. 149:746-752.
10. Hagemann, M., S. Richter, E. Zuther, and A. Schoor. 1996. Characterization of a glucosylglycerol-phosphate accumulating, salt-sensitive mutant of the cyanobacterium Synechocystis sp. strain PCC6803. Arch. Microbiol. 166:83-91.
11. Hengge-Aronis, R., W. Klein, R. Lange, M. Rimmerle, and W. Boos. 1991. Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli. J. Bacteriol. 173:7918-7924.
12. Jeanjean, R., B. Onana, G. A. Pesehek, and F. Joset. 1990. Mutants of the cyanobacterium Synechocystis PCC 6803 impaired in respiration and unable to tolerate high salt concentrations. FEMS Microbiol. Lett. 68:125-130.
13. Joset, F., R. Jeanjean, and M. Hagemann. 1996. Dynamics of the response of cyanobacteria to salt stress: deciphering the molecular events. Physiol. Plant. 96:738-744.
14. Kaasen, I., P. Falkenberg, O. B. Styrvold, and A. Strøm. 1992. Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by KatF (AppR). J. Bacteriol. 174:889-898.
15. Kaasen, I., J. McDougall, and A. Strøm. 1994. Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/ phosphatase complex. Gene 145:9-15.
16. Klein, W., U. Ehmann, and W. Boos. 1991. The repression of trehalose transport and metabolism in Escherichia coli by high osmolarity is mediated by trehalose-6-phosphate phosphatase. Res. Microbiol. 142:359-371.
17. Kratz, W. A., and J. Myers. 1955. Nutrition and growth of several blue-green algae. Am. J. Bot. 42:282-287.
18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
19. Mikkat, S., M. Hagemann, and A. Schoor. 1996. Active transport of glucosylglycerol is involved in salt adaptation of the cyanobacterium Synechocystis sp. strain PCC 6803. Microbiology 142:1725-1732.
20. Onana, B., R. Jeanjean, and F. Joset. 1994. A gene, stpA, involved in the establishment of salt tolerance in the cyanobacterium Synechocystis PCC 6803. Russ. Plant Physiol. 41:1176-1183.
21. Reddy, K. J., R. Webb, and L. A. Sherman. 1990. Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge. BioTechniques 8:250-251.
22. Reed, R. H., and W. D. P. Stewart. 1985. Osmotic adjustment and organic solute accumulation in unicellular cyanobacteria from freshwater and marine habitats. Mar. Biol. 88:1-9.
23. Reed, R. H., L. J. Borowitzka, M. A. MacKay, J. A. Chudek, R. Foster, S. R. C. Warr, D. J. Moore11W. D. P. Stewart. 1986. Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiol. Rev. 39:51-56.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Schoor, A., N. Erdmann, U. Effmert, and S. Mikkat. 1995. Determination of the cyanobacterial osmolyte glucosylglycerol by high-performance liquid chromatography. J. Chromatogr. 704:89-97.
26. Stal, L. J., and R. H. Reed. 1987. Low-molecular mass carbohydrate accumulation in cyanobacteria from a marine microbial mat in response to salt. FEMS Microbiol. Ecol. 45:305-312.
27. Tel-Or, E., S. Spath, C. Packer, and R. J. Mehlhorn. 1986. Carbon-13 NMR studies of salt shock-induced carbohydrate turnover in the marine cyanobacterium Agmenellum quadntplicatutn. Plant Physiol. 82:646-652.
28. Van Etten, R. L., R. Davidson, P. E. Stevis, H. MacArthur, and D. L. Moore. 1991. Covalent structure, disulfide bonding, and identification of reactive surface and active site residues of human prostatic acid phosphatase. J. Biol. Chem. 266:2313-2319.
29. Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived system for the insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259.
30. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103-119.