Characterization of two pituitary GH3 cell sublines partially resistant to apoptosis induction by okadaic acid

Biochemical Pharmacology

Volumn 54, Issue 9, 1997, Pages 967-971

  Ritz, Vera ^a   Marwitz, John ^a   Richter, Eva ^a   Ziemann, Christina ^a   Quentin, Iris ^a   Steinfelder, Hans Jürgen ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

GH3 cells; Multidrug resistance (MDR) phenotype; Okadaic acid; Ser thr phosphatases

Indexed keywords

OKADAIC ACID; PHOSPHATASE; DNA FRAGMENT; GLYCOPROTEIN P;

APOPTOSIS; ARTICLE; CELL STRAIN GH3; DRUG RESISTANCE; HUMAN; HUMAN CELL; HYPOPHYSIS CELL; IMMUNOBLOTTING; MULTIDRUG RESISTANCE; PRIORITY JOURNAL; SURVIVAL RATE; ANIMAL; CELL CULTURE; DRUG EFFECT; HYPOPHYSIS TUMOR; PATHOLOGY; PHYSIOLOGY; RAT;

ANIMALS; APOPTOSIS; DNA FRAGMENTATION; DRUG RESISTANCE, MULTIPLE; OKADAIC ACID; P-GLYCOPROTEIN; PITUITARY NEOPLASMS; RATS; TUMOR CELLS, CULTURED;

EID: 0030613794     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(97)00397-3     Document Type: Article

References (21)

1. Cohen P, The structure and regulation of protein phosphatases. Annu Rev Biochem 58: 453-508, 1989.
2. Honkanen RE, Zwiller J, Daily SL, Khatra BS, Dukelow M and Boynton AL, Identification, purification, and characterization of a novel serine/threonine protein phosphatase from bovine brain. J Biol Chem 266: 6614-6619, 1991.
3. Brewis ND, Street AJ, Prescott AR and Cohen PT, PPX, a novel protein serine/threonine phosphatase localized to centrosomes. EMBO J 12: 987-996, 1993.
4. Becker W, Kentrup H, Klumpp S, Schultz JE and Joost HG, Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain. J Biol Chem 269: 22586-22592, 1994.
5. Chen MX, McPartlin AE, Brown L, Chen YH, Barker HM and Cohen PT, A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus. EMBO J 13: 4278-4290, 1994.
6. Tachibana K, Scheuer PJ, Tsukitani Y, Kikuchi H, Van Eugen D, Clardy J, Gopichand Y and Schmitz FJ, Okadaic acid, a cytotoxic polyether toxin from two marine sponges of the genus Halichondria. J Am Chem Soc 103: 2469-2471, 1981.
7. Kato Y, Fusetani N, Matsunaga S and Hashimoto K, Calyculins, potent antitumour metabolites from the marine sponge Discodermia calyx: biological activities. Drugs Exp Clin Res 14: 723-728, 1988.
8. Honkanen RE, Codispoti BA, Tse K, and Boynton AL, Characterization of natural toxins with inhibitory activity against serine/threonine protein phosphatases. Toxicon 32: 339-350, 1994.
9. Suganuma M, Fujiki H, Suguri H, Yoshizawa S, Hirota M, Nakayasu M, Ojika M, Wakamatsu K, Yamada K and Sugimura T, Okadaic acid: an additional non-phorbol-12-tetra-decanoate-13-acetate-type tumor promoter. Proc Natl Acad Sci USA 85: 1768-1771, 1988.
10. Kim SJ, Lafyatis R, Kim KY, Angel P, Fujiki H, Karin M, Sporn MB and Roberts AB, Regulation of collagenase gene expression by okadaic acid, an inhibitor of protein phosphatases. Cell Regul 1: 269-278, 1990.
11. Bøe R, Gjertsen BT, Vintermyr OK, Houge G, Lanotte M and Døskeland SO, The protein phosphatase inhibitor okadaic acid induces morphological changes typical of apoptosis in mammalian cells. Exp Cell Res 195: 237-246, 1991.
12. Kiguchi K, Glesne D, Chubb CH, Fujiki H and Huberman E, Differential induction of apoptosis in human breast tumor cells by okadaic acid and related inhibitors of protein phosphatases 1 and 2A. Cell Growth Differ 5: 995-1004, 1994.
13. Sakai R, Ikeda I, Kitani H, Fujiki H, Takaku F, Rapp U, Sugimura T and Nagao M, Flat reversion by okadaic acid of raf and ret-Il transformants. Proc Natl Acad Sci USA 86: 9946-9950, 1989.
14. Tashjian, AH, Clonal strains of hormone-producing pituitary cells. Meth Enzymol 58: 527-535, 1979.
15. Darzynkiewicz Z, Li X and Gong J, Assays of cell viability. Discrimination of cells dying by apoptosis. Methods Cell Biol 41: 15-38, 1994.
16. Simpson IA, Yver DR, Hissin PJ, Wardzala LJ, Karnieli E, Salans LB and Cushman SW, Insulin-stimulated translocation of glucose transporters in the isolated rat adipose cells: characterization of subcellular fractions. Biochim Biophys Acta 763: 393-407, 1983.
17. Arends MJ, Morris RG and Wyllie AH, Apoptosis. The role of the endonuclease. Am J Pathol 136: 593-608, 1990.
18. Gottesman MM and Pastan I, Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu Rev Biochem 62:385-427, 1993.
19. Endicott JA and Ling V, The biochemistry of P-glycoprotein-mediated multidrug resistance. Annu Rev Biochem 58: 137-171, 1989.
20. Hansen MB, Nielsen SE and Berg K, Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 119: 203-210, 1989.
21. 3 cells by inhibition of serine/threonine phosphatases. Naunyn-Schmiedeberg's Arch Pharmacol 349 (Suppl.): R6, 1994.