Mossbauer study of 4-hydroxybutyryl-CoA dehydratase: Probing the role of an iron-sulfur cluster in an overall non-redox reaction

European Journal of Biochemistry

Volumn 248, Issue 2, 1997, Pages 380-384

  Müh, Ute ^a   Buckel, Wolfgang ^a   Bill, Eckhard ^b  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b MAX PLANCK INSTITUTE FOR CHEMICAL ENERGY CONVERSION   (Germany)

Author keywords

4 Hydroxybutyryl CoA dehydrate; Clostridium aminobutyricum; FAD; Iron sulfur cluster; Mossbauer spectroscopy

Indexed keywords

HYDROLYASE; IRON; 4 HYDROXYBUTYRYL COA DEHYDRATASE; 4-HYDROXYBUTYRYL-COA DEHYDRATASE; SULFUR;

ARTICLE; CLOSTRIDIUM; ENZYME ACTIVITY; ENZYME STRUCTURE; ISOTOPE LABELING; MOSSBAUER SPECTROSCOPY; PRIORITY JOURNAL; AIR; CHEMISTRY; ENZYME ACTIVATION; METABOLISM; OXIDATION REDUCTION REACTION;

CLOSTRIDIUM; CLOSTRIDIUM AMINOBUTYRICUM;

AIR; ENZYME ACTIVATION; HYDRO-LYASES; IRON; OXIDATION-REDUCTION; SPECTROSCOPY, MOSSBAUER; SULFUR;

EID: 0030613554     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00380.x     Document Type: Article

References (18)

1. Arciero, D. M., Lipscomb, J. D., Huynh, B. H., Kent, T. A. & Münck, E. (1983) EPR and Mössbauer studies of protocatechuate 4,5-dioxygenase, J. Biol. Chem. 258, 14981-14991.
2. Beinert, H. & Kennedy, M. C. & Stout, D. C. (1996) Aconitase as iron-sulfur protein, enzyme, and iron-regulatory protein. Chem. Rev. 96, 2335-2374.
3. Buckel, W. (1996) Unusual dehydrations in anaerobic bacteria: Considering ketyls (radical anions) as reactive intermediates in enzymatic reactions, FEBS Lett. 389, 20-24.
4. 12-dependent enzymes. Chem. Soc. Rev. 25, 329-337.
5. Cunningham, R. P., Asahara, H., Bank, J. F., Scholes, C. P., Salerno, J. C., Surerus, K., Münck, E., McCracken, J., Peisach, J. & Emptage, M. H. (1989) Endonuclease III is an iron-sulfur protein, Biochemistry 28, 4450-4455.
6. Dickson, D. P. E. (1984) Applications to biological systems, in Mössbauer spectroscopy applied to inorganic chemistry (Long, G. J., ed.) pp. 339-389, Plenum Press, New York.
7. 12-dependent diol dehydratase; stereochemical and model studies: the bound radical mechanism. Coord. Chem. Rev. 54, 1-22.
8. Fish, W. W. (1988) Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples, Methods Enzymol. 158, 357-364.
9. Johnson, M. K. (1994) Iron-sulfur proteins, in Encyclopedia of inorganic chemistry (King, R. B., ed.) pp. 1896-1915, Wiley, Chichester.
10. Kent, T. A., Huynh, B. H. & Münck, E. (1980) Iron-sulfur proteins: spin-coupling model for three-iron clusters, Proc. Natl Acad. Sci. USA 77, 6574-6576.
11. Müh, U., Cinkaya, I., Albracht, S. P. J. & Buckel, W. (1996) 4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: Characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction, Biochemistry 35, 11710-11718.
12. Scherf, U. & Buckel, W. (1991) Purification and properties of 4-hydroxybutyrate coenzyme a transferase from Clostridium aminobutyricum, Appl. Environ. Microbiol. 57, 2699-2702.
13. 2-isomerase from Clostridiuin aminobutyricum. Eur. J. Biochem. 215, 421-429.
14. Schulz, C. E., Nyman, P. & Debrunner, P. G. (1987) Spin fluctuations of paramagnetic iron centers in proteins and model complexes: Mössbauer and EPR results, J. Chem. Phys. 87, 5077-5091.
15. Switzer, R. L. (1989) Non-redox roles for iron-sulfur clusters in enzymes, BioFactors 2, 77-86.
16. Trautwein, A. X., Bill, E., Bominaar, E. L. & Winkler, H. (1991) Iron-containing proteins and related analogs complementary Mössbauer, EPR and magnetic susceptibility studies, Struct. Bonding 78, 1-95.
17. Vollmer, S. J., Switzer, R. L. & Debrunner, P. G. (1983) Oxidation-reduction properties of the iron-sulfur cluster in Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase, J. Biol. Chem. 258, 14284-14293.
18. Wickman, H. H., Klein, M. P. & Shirley, D. A. (1966) Paramagnetic hyperfine structure and relaxation effects in Mössbauer spectra: Fe-57 in ferrichrom A, Phys. Rev. 152, 345-357